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[edit] Construction details

p3E-MTpA was made by PCR-amplifying the 6x myc tag and SV40 late polyadenylation signal from pCS2+MT, using primers to add att sequences, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct.

[edit] Sequence

Annotated sequence, Genbank format:
p3E-MTpA Genbank

FASTA file with the full-length sequence as well as sequences of individual components:
p3E-MTpA sequence

[edit] Crude map

Screenshot from Sequencher showing locations of components:

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