P3E-mCherrypA

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[edit] Construction details

p3E-mCherrypA was made by PCR amplification of mCherry and an SV40 late polyadenylation signal from a pCS2+-based construct, using primers to add att sites, followed by a BP reaction. Sequencing finds a single base PCR error, encoding an E7D mutation in mCherry. Functional tests of C-terminal fusion proteins made with this construct show that they yield fluorescent protein.

The mCherry sequence includes a start codon but not a good Kozak consensus (unlike p3E-EGFP-pA, which has both).

[edit] Sequence

Annotated sequence, Genbank format:
p3E-mCherrypA Genbank

FASTA file with the full-length sequence as well as sequences of individual components:
p3E-mCherrypA sequence

[edit] Crude map

Screenshot from Sequencher showing locations of components:
Image:p3E-mCherrypA.png

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