From Tol2Kit

Jump to: navigation, search

[edit] Construction details

p3E-polyA was made by PCR amplification of the SV40 late polyadenylation signal from pCS2+, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct.

[edit] Sequence

Annotated sequence, Genbank format:
p3E-polyA Genbank

FASTA file with the full-length sequence as well as sequences of individual components:
p3E-polyA sequence

[edit] Crude map

Screenshot from Sequencher showing locations of components:

Personal tools