P3E-polyA

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[edit] Construction details

p3E-polyA was made by PCR amplification of the SV40 late polyadenylation signal from pCS2+, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct.

[edit] Sequence

Annotated sequence, Genbank format:
p3E-polyA Genbank

FASTA file with the full-length sequence as well as sequences of individual components:
p3E-polyA sequence

[edit] Crude map

Screenshot from Sequencher showing locations of components:
Image:p3E-polyA.png

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