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[edit] Construction details

p5E-Fse-Asc was made by PCR of two overlapping primers with att sites and the Fse and Asc recognition sites, followed by a BP reaction. The insert consists of:


that is, an Fse then an Asc site, separated by a 4 bp spacer. Sequencing confirms that all bases of the insert are correct.

[edit] Sequence

Annotated sequence, Genbank format:
p5E-Fse-Asc Genbank

FASTA file with the full-length sequence as well as sequences of individual components:
p5E-Fse-Asc sequence

[edit] Crude map

Screenshot from Sequencher showing locations of components:

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