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[edit] Construction details

bactin2 genomic sequence was derived from a 5.3 kb clone (gift of Ken Poss), which was derived in turn from a 10 kb upstream construct from Shin-ichi Higashijima.

p5E-bactin2 was made by PCR of the bactin2 upstream genomic sequence (using primers to add att sites), followed by a BP reaction. Upstream sequence includes 3.8 kb upstream of transcriptional start, a small 5' UTR exon, a 1.4 kb intron, and then 38 bp of 5' UTR, terminating immediately before the native start codon. Sequencing confirms that the ends of the insert are correct.

[edit] Reference

Higashijima S, Okamoto H, Ueno N, Hotta Y, Eguchi G. (1997) Dev Biol.15;192:289-99. "High-frequency generation of transgenic zebrafish which reliably express GFP in whole muscles or the whole body by using promoters of zebrafish origin."

[edit] Sequence

Annotated sequence, Genbank format:
p5E-bactin2 Genbank

FASTA file with the full-length sequence as well as sequences of individual components:
p5E-bactin2 sequence

[edit] Crude map

Screenshot from Sequencher showing locations of components:

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