From Tol2Kit
(Redirected from P5E-hsp70 Genbank)
LOCUS 222.p5E-hsp70 4163 bp ds-DNA circular 09-DEC-2007
DEFINITION
REFERENCE 1
AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS,
Parant JM, Yost HJ, Kanki JP, Chien CB.
TITLE The Tol2kit: a multisite gateway-based construction kit for Tol2
transposon transgenesis constructs.
JOURNAL Developmental Dynamics 236(11):3088-99 (2007).
PUBMED 17937395
COMMENT 222.p5E-hsp70l, Tol2kit v1.0
COMMENT ApEinfo:methylated:1
FEATURES Location/Qualifiers
misc_recomb 593..688
/label=attL4
/ApEinfo_fwdcolor=#5d40ff
/ApEinfo_revcolor=#5d40ff
misc_recomb 2209..2365
/label=attR1
/ApEinfo_fwdcolor=#3fd0ff
/ApEinfo_revcolor=#3fd0ff
misc_feature 2213..2226
/label=att1_shared
/ApEinfo_fwdcolor=#32a6cc
/ApEinfo_revcolor=#32a6cc
misc_feature 668..688
/label=att4_shared
/ApEinfo_fwdcolor=#4a33cc
/ApEinfo_revcolor=#4a33cc
promoter 691..2207
/label=hsp70l promoter (AF158020)
/ApEinfo_fwdcolor=#ffffbf
/ApEinfo_revcolor=#ffffbf
rep_origin 3487..4160
/label=pUC ori
/ApEinfo_fwdcolor=#999999
/ApEinfo_revcolor=#999999
CDS 2557..3366
/label=kanR
/ApEinfo_fwdcolor=#ff7f3f
/ApEinfo_revcolor=#ff7f3f
primer_bind complement(2424..2444)
/label=M13R
/ApEinfo_fwdcolor=#777777
/ApEinfo_revcolor=#777777
primer_bind 537..552
/label=M13F
/ApEinfo_fwdcolor=#777777
/ApEinfo_revcolor=#777777
promoter complement(2401..2420)
/label=T7
/ApEinfo_fwdcolor=#777777
/ApEinfo_revcolor=#777777
terminator 268..295
/label=T2
/ApEinfo_fwdcolor=#bbbbbb
/ApEinfo_revcolor=#bbbbbb
terminator 427..470
/label=T1
/ApEinfo_fwdcolor=#bbbbbb
/ApEinfo_revcolor=#bbbbbb
ORIGIN
1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
541 AACGACGGCC AGTCTTAAGC TCGGGCCCGC GTTAACGCTA CCATGGAGCT CCAAATAATG
601 ATTTTATTTT GACTGATAGT GACCTGTTCG TTGCAACAAA TTGATAAGCA ATGCTTTTTT
661 ATAATGCCAA CTTTGTATAG AAAAGTTGCT TCAGGGGTGT CGCTTGGTTA TTTCCAAAAA
721 TCAAATTAAT TTTATTAAAC TATTAGAACG AGCATGTTTT GTCTATATGC TACAGAAGAT
781 AAAAAATAAT AGGAGTTAAC AGTTATAAAA CAACACACTT TGTTTCTATT GATTGTTGAC
841 CACACTGGGG TCTCATTAAG TTAGATTAAA GACACACTAA CTGGGTCAAA AGCAGCAGAT
901 TGATTTCATA GCACCAGGGT AAACTTTCTA ACACTTTTAC GGCAATCATA TACATTAAAA
961 TTAAATACAG ACCACGACTG AACAAGGAGG ATGATCTCCA ATATTAAACA AAGAGACTTG
1021 TGCCTATTTC TCTGAGGGTA AACATGACCT CTCAAGTTAG CAAGTTGGTT TTAACACTAC
1081 AAAAATAGTT AAGACTGCAA TCCCAGAATA AAGTATTGGT TTTAACCAAT CAATATAGTA
1141 CAGTAAACAT CCATTTGTTT TGTTGAAACG TTAAACAAAT CTGACCAAAG CTATTAGCTT
1201 ATATAAAACA GGTTTGCCTT CTATGTAGCT GAAAACACCA CAGGCCCGAT TTTGCTACTG
1261 TGTAAAACAT TTCAGCAAGA TTTTTTTATT GCATTTTTTT TTACTGAATC GTTCAAACAT
1321 TTTATCATTT TAGTTTGTTC ATTCATTGCA ACTGGAAAAA CAACACATCA CACAACCGCA
1381 CATATTTCAG CAATAAGTAC AATAAAACAC TCAAATAAAA AAAACATTTT AAATCTCTTT
1441 GTATTTTTGA CCGCTGTTTC GCGTAATTTC ACGGTAAAAC TCTGGAAATC TCCACTACAT
1501 TCCTCTCAGC GGCTCCTCTC AATGACAGCT GAAGAAGTGA CGCGGCTGCC TGCTGTGTTT
1561 TGATTGGTCG AATTCACTGG AGGCTTCCAG AACAGTGTAG AGTCTGAACG GGTGCGCGCT
1621 CTGCTGTATT TAAAGGGCGA AAGAGAGACC GCAGAGAAAC TCAACCGAAG AGAAGCGACT
1681 TGACAAAGAA GAAAAGAGCA GCCTGACAGG ACTTTTTCCC CGACGAGGTG TTTATTCGCT
1741 CTATTTAAGA ATCTACTGTA AGGTAAGTCT CAATATATTG TACTCTATTG GCTAATCAGA
1801 ATTATATAGA GATTATATGT ACTTAATGTC AAAAAATCAA CTTTGTATAT GTAATCTTTT
1861 TACATGTGGA CTGCCTATGT TCATCTTATT TTAGGTCTAC TAGAAAATTA TATTTCCCGT
1921 TTTCACAATA AGGATTTTTA AAAAAAGCAA TGAACAGACG GGCATTTACT TTATGTTGCT
1981 GACATTATTT TATATGAGCA TAATAACCAT AAATACTAGC AAATGTCCTA AATGAATTTG
2041 TGTTAATGTT GTCTACAAAA GAAAATTAGC GTTTTACTTG TACAACTAAT AATAACTTGG
2101 TTATTAAGAG AATTTCACTT GTTGACTAGA AAAATCCTTT CATAATGAAA CAATTGCACC
2161 ATAAATTGTA TAAATATAAA ATTAATTCTA ATTGTTTTTT TTTCCTGCCA AGTTTGTACA
2221 AAAAAGTTGA ACGAGAAACG TAAAATGATA TAAATATCAA TATATTAAAT TAGATTTTGC
2281 ATAAAAAACA GACTACATAA TACTGTAAAA CACAACATAT GCAGTCACTA TGAACCAACT
2341 ACTTAGATGG TATTAGTGAC CTGTAGAATT CGAGCTCTAG AGCTGCAGGG CGGCCGCGAT
2401 ATCCCCTATA GTGAGTCGTA TTACATGGTC ATAGCTGTTT CCTGGCAGCT CTGGCCCGTG
2461 TCTCAAAATC TCTGATGTTA CATTGCACAA GATAAAAATA TATCATCATG AACAATAAAA
2521 CTGTCTGCTT ACATAAACAG TAATACAAGG GGTGTTATGA GCCATATTCA ACGGGAAACG
2581 TCGAGGCCGC GATTAAATTC CAACATGGAT GCTGATTTAT ATGGGTATAA ATGGGCTCGC
2641 GATAATGTCG GGCAATCAGG TGCGACAATC TATCGCTTGT ATGGGAAGCC CGATGCGCCA
2701 GAGTTGTTTC TGAAACATGG CAAAGGTAGC GTTGCCAATG ATGTTACAGA TGAGATGGTC
2761 AGACTAAACT GGCTGACGGA ATTTATGCCT CTTCCGACCA TCAAGCATTT TATCCGTACT
2821 CCTGATGATG CATGGTTACT CACCACTGCG ATCCCCGGAA AAACAGCATT CCAGGTATTA
2881 GAAGAATATC CTGATTCAGG TGAAAATATT GTTGATGCGC TGGCAGTGTT CCTGCGCCGG
2941 TTGCATTCGA TTCCTGTTTG TAATTGTCCT TTTAACAGCG ATCGCGTATT TCGTCTCGCT
3001 CAGGCGCAAT CACGAATGAA TAACGGTTTG GTTGATGCGA GTGATTTTGA TGACGAGCGT
3061 AATGGCTGGC CTGTTGAACA AGTCTGGAAA GAAATGCATA AACTTTTGCC ATTCTCACCG
3121 GATTCAGTCG TCACTCATGG TGATTTCTCA CTTGATAACC TTATTTTTGA CGAGGGGAAA
3181 TTAATAGGTT GTATTGATGT TGGACGAGTC GGAATCGCAG ACCGATACCA GGATCTTGCC
3241 ATCCTATGGA ACTGCCTCGG TGAGTTTTCT CCTTCATTAC AGAAACGGCT TTTTCAAAAA
3301 TATGGTATTG ATAATCCTGA TATGAATAAA TTGCAGTTTC ATTTGATGCT CGATGAGTTT
3361 TTCTAATCAG AATTGGTTAA TTGGTTGTAA CACTGGCAGA GCATTACGCT GACTTGACGG
3421 GACGGCGCAA GCTCATGACC AAAATCCCTT AACGTGAGTT ACGCGTCGTT CCACTGAGCG
3481 TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC
3541 TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG
3601 CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC
3661 CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC
3721 CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC
3781 GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT
3841 TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT
3901 GAGCATTGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC
3961 GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT
4021 TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA
4081 GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT
4141 TGCTGGCCTT TTGCTCACAT GTT
//