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Construction details

p5E-hsp70l was made by PCR amplification of the 1.5 kb hsp70l promoter (Genbank AF158020), using primers to add att sites, followed by a BP reaction. Note that hsp70l is the official ZFIN gene name for the gene originally named hsp70 by Halloran et al., 2000. Thanks to Ceri van Slyke of ZFIN for pointing this out.

Sequencing shows a single base mutation in the hsp70l promoter (T1068G), but this does not appear to be a critical base as constructs made with this clone show normal induction by heatshock.


Annotated sequence, Genbank format:
p5E-hsp70l Genbank

FASTA file with the full-length sequence as well as sequences of individual components:
p5E-hsp70l sequence

Crude map

Screenshot from Sequencher showing locations of components: