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[edit] Construction details

pCS2FA-transposase was made by PCRing the Tol2 transpoase cds from the original Kawakami clone, adding restriction sites, then conventionally subcloned into pCS2FA, a version of pCS2+ modified by adding FseI and AscI sites. Sequencing confirms that all bases of the insert are correct.

The original transposase clone included start and stop codons upstream of the transposase cds, and a polyA sequence (AAAAA) rather than a polyadenylation signal.

[edit] Sequence

Annotated sequence, Genbank format:
pCS2FA-transposase Genbank

FASTA file with the full-length sequence as well as sequences of individual components:
pCS2FA-transposase sequence

[edit] Crude map

Screenshot from Sequencher showing locations of components:

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