From Tol2Kit
Construction details
pDestTol2CG was made by PCRing the cmlc2:egfp-pA cassette, adding BglII sites in the primers, then cloning into the unique BglII site of pDestTol2pA and selecting for reverse orientation relative to the gate and existing polyA signal. The cmlc2:egfp cassette has not been sequenced, but works in functional tests.
Crudely annotated sequence
FASTA file with the full-length sequence as well as sequences of individual components:
pDestTol2CG sequence
Edited 7 June 2007 to add six bases (a KpnI site that was missing).
Crude map
Screenshot from Sequencher showing locations of components:
