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Construction details

pDestTol2CG was made by PCRing the cmlc2:egfp-pA cassette, adding BglII sites in the primers, then cloning into the unique BglII site of pDestTol2pA and selecting for reverse orientation relative to the gate and existing polyA signal. The cmlc2:egfp cassette has not been sequenced, but works in functional tests.

Crudely annotated sequence

FASTA file with the full-length sequence as well as sequences of individual components:
pDestTol2CG sequence

Edited 7 June 2007 to add six bases (a KpnI site that was missing).

Crude map

Screenshot from Sequencher showing locations of components: