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[edit] Construction details

pDestTol2CG2 was made from pDestTol2CG by cutting with AfeI/NgoMI and religating; this removed ~2kb comprising medaka tyrosinase genomic sequence and part of the f1 ori. This reduced the size of the construct without affecting its functionality.

[edit] Sequence

Annotated sequence, Genbank format:
pDestTol2CG2 Genbank

FASTA file with the full-length sequence as well as sequences of individual components):
pDestTol2CG2 sequence

Edited 7 June 2007 to add six bases (a KpnI site that was missing).

[edit] Crude map

Screenshot from Sequencher showing locations of components:

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