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Construction details

pME-EGFP was made by PCR amplification of the EGFP insert from a pCS2-based plasmid, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct.

Note that the EGFP sequence is followed by 60 bp of polylinker sequence from pCS2+ before the stop codon. We have confirmed functionally that this cassette produces fluorescent protein.


Annotated sequence, Genbank format:
pME-EGFP Genbank

FASTA file with the full-length sequence as well as sequences of individual components:
pME-EGFP sequence

Crude map

Screenshot from Sequencher showing locations of components: