PME-Gal4VP16

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[edit] Construction details

pME-Gal4VP16 was made by PCR amplification of Gal4VP16 from clones made by Reinhard Koester (described in Koester and Fraser, Dev Biol. 2001), using primers that add att sites, followed by a BP reaction. Sequencing finds a single silent mutation, G1114T.

In this construct, the Gal4 DNA binding domain is fused to VP16[413-470], a partially-truncated VP16 transactivation domain. This fusion has reduced toxicity compared to the original Gal4VP16[413-490] from Sadowski et al. 1988. See Ogura et al., Dev Dyn 238:641, 2009 for comparisons of different Gal4VP16 fusions.

[edit] Sequence

Annotated sequence, Genbank format:
pME-Gal4VP16 Genbank

FASTA file with the full-length sequence as well as sequences of individual components:
pME-Gal4VP16 sequence

[edit] Crude map

Screenshot from Sequencher showing locations of components:
Image:pME-Gal4VP16.png

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