PME-H2AmCherry

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[edit] Construction details

pME-H2AmCherry was made by using PCR to fuse zebrafish H2A.F/Z (cloned by RT-PCR; official ZFIN gene name h2afv) to the N terminus of mCherry, using primers that add att sites, followed by a BP reaction. Sequencing finds a single silent mutation, A883G.

[edit] Sequence

Annotated sequence, Genbank format:
pME-H2AmCherry Genbank

FASTA file with the full-length sequence as well as sequences of individual components:
pME-H2AmCherry sequence

[edit] Crude map

Screenshot from Sequencher showing locations of components:
Image:pME-H2AmCherry.png

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