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[edit] Construction details

pME-nlsEGFP was made by PCR amplification of an nls-EGFP (EGFP with nuclear-localization signal) cassette, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct.

[edit] Sequence

Annotated sequence, Genbank format:
pME-nlsEGFP Genbank

FASTA file with the full-length sequence as well as sequences of individual components:
pME-nlsEGFP sequence

[edit] Crude map

Screenshot from Sequencher showing locations of components:

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