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[edit] Construction details

pME-nlsmCherry was made by PCR amplification of an nls-mCherry (mCherry with nuclear-localization signal) cassette, using primers that add att sites, followed by a BP reaction.

Sequencing finds a single PCR error in the insert, encoding an H30R mutation in mCherry. However, expression constructs made with this cassette show appropriate function (nuclear-localized fluorescence).

[edit] Sequence

Annotated sequence, Genbank format:
pME-nlsmCherry Genbank

FASTA file with the full-length sequence as well as sequences of individual components:
pME-nlsmCherry sequence

[edit] Crude map

Screenshot from Sequencher showing locations of components:

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