From Tol2Kit
m (PDestTol2pA sequence moved to PDestTol2pA2 sequence: typo in original name) |
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| Line 1: | Line 1: | ||
<pre> | <pre> | ||
| − | > | + | >pDestTol2pA2 |
CCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTT | CCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTT | ||
TGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCC | TGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCC | ||
| Line 32: | Line 32: | ||
GCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATC | GCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATC | ||
ACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGT | ACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGT | ||
| − | + | AAACCTTAAACTGCATTTCACCAGCCCCTGTTCTCGTCGGCAAAGGAGCC | |
GTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCG | GTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCG | ||
CTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTG | CTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTG | ||
| Line 239: | Line 239: | ||
TATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCT | TATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCT | ||
CGTTCAACTTTATTATACATAGTTG | CGTTCAACTTTATTATACATAGTTG | ||
| − | > | + | >attB3/L3/R3_shared |
| − | + | CAACTTTATTATACA | |
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>cmR | >cmR | ||
TTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCA | TTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCA | ||
| Line 298: | Line 264: | ||
TCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAA | TCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAA | ||
CTGCAT | CTGCAT | ||
| + | >ccdB_from_pDEST_R4-R3_(1_mutation) | ||
| + | ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTT | ||
| + | TGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGA | ||
| + | TCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCCCCCGTGAACTT | ||
| + | TACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGA | ||
| + | TATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCA | ||
| + | GCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGA | ||
| + | ATATAA | ||
>attR4 | >attR4 | ||
CAACTTTGTATAGAAAAGTTGAACGAGAAACGTAAAATGATATAAATATC | CAACTTTGTATAGAAAAGTTGAACGAGAAACGTAAAATGATATAAATATC | ||
AATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAA | AATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAA | ||
AACACAACATATCCAGTCACTATGG | AACACAACATATCCAGTCACTATGG | ||
| + | >attB4/L4/R4_shared | ||
| + | CAACTTTGTATAGAAAAGTTG | ||
>M13R | >M13R | ||
GGAAACAGCTATGACCATGA | GGAAACAGCTATGACCATGA | ||
| Line 324: | Line 300: | ||
TACTTTTACTTTTAATTGAGTAAAATTTTCCCTAAGTACTTGTACTTTCA | TACTTTTACTTTTAATTGAGTAAAATTTTCCCTAAGTACTTGTACTTTCA | ||
CTTGAGTAAAATTTTTGAGTACTTTTTACACCTCTG | CTTGAGTAAAATTTTTGAGTACTTTTTACACCTCTG | ||
| − | |||
| − | |||
>3'_TIR | >3'_TIR | ||
TACTTTTTACACCTCTG | TACTTTTTACACCTCTG | ||
| Line 338: | Line 312: | ||
CAGGTCATTGCACAACACCAGAAATGCCCTCTGATCTGCAAAAGACGTGA | CAGGTCATTGCACAACACCAGAAATGCCCTCTGATCTGCAAAAGACGTGA | ||
ATATCTGTTCAGACACCCATATCCACTCTGTTCCACACAGG | ATATCTGTTCAGACACCCATATCCACTCTGTTCCACACAGG | ||
| + | >f1_(+)_ori_fragment | ||
| + | GTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCC | ||
| + | ACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAA | ||
>pBSII_SK+_5’_of_DraIII | >pBSII_SK+_5’_of_DraIII | ||
CCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTT | CCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTT | ||
| Line 344: | Line 321: | ||
GGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGA | GGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGA | ||
AAAACCGTCTATCAGGGCGATGGCCCAC | AAAACCGTCTATCAGGGCGATGGCCCAC | ||
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</pre> | </pre> | ||
Revision as of 23:08, 19 March 2007
>pDestTol2pA2 CCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTT TGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCC TTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTT GGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGA AAAACCGTCTATCAGGGCGATGGCCCACGCTGGCTAAGAACTCATCAGCC TCCCCGGTCCATCTACCCACGTACCAATGCACCAATTGGCCACAATGACG GCTACTACATGGTGCCATTCCTTCCTCTTTATAGGAATGGAGACTACCTC CTGTCCAACAAGGCTCTTGGATACGAGTACGCCTACCTGTTGGACCCAGG TCATTGCACAACACCAGAAATGCCCTCTGATCTGCAAAAGACGTGAATAT CTGTTCAGACACCCATATCCACTCTGTTCCACACAGGTCAGAGGTTTGTC CAGGAGTTCTTGACAGAGGTGTAAAAAGTACTCAAAAATTTTACTCAAGT GAAAGTACAAGTACTTAGGGAAAATTTTACTCAATTAAAAGTAAAAGTAT CTGGCTAGAATCTTACTTGAGTAAAAGTAAAAAAGTACTCCATTAAAATT GTACTTGAGTATTAAGGAAGTAAAAGTAAAAGCAAGAAAGAAAACTAGAG ATTCTTGTTTAAGCTTTTAATCTCAAAAAACATTAAATGAAATGCATACA AGGTTTTATCCTGCTTTAGAACTGTTTGTATTTAATTATCAAACTATAAG ACAGACAATCTAATGCCAGTACACGCTACTCAAAGTTGTAAAACCTCAGA TTTAACTTCAGTAGAAGCTGATTCTCAAAATTGTTAGTGTCAAGCCTAGC TCTTTTGGGGCTGAAAAGCAATCCTGCAGTGCTGAAAAGCCTCTCACAGG CAGCCGATGCGGGAAGAGGTGTATTAGTCTTGATAGAGAGGCTGCAAATA GCAGGAAACGTGAGCAGAGACTCCCTGGTGTCTGAAACACAGGCCAGATG GGCCCTCGAGCAGGAAACAGCTATGACCATGATTACGCCAAGCTATCAAC TTTGTATAGAAAAGTTGAACGAGAAACGTAAAATGATATAAATATCAATA TATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACA CAACATATCCAGTCACTATGGTCGACCTGCAGACTGGCTGTGTATAAGGG AGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATG TCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGAC CGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGA TATGCACCACCGGGTAAAGTTCACGGGGGACTTTATCTGACAGCAGACGT GCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATC ACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGT AAACCTTAAACTGCATTTCACCAGCCCCTGTTCTCGTCGGCAAAGGAGCC GTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCG CTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTG CTTACCGAACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAG CTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCATACCTCTT TTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAA ATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATC CTCTAGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCA TTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTG AATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCA TGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCA AAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTC AATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACAT CTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTC CAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGG GTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGA ATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGA TAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATC CAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCT CAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCA GTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGACGG ATCCTAACTCAAAATCCACACATTATACGAGCCGGAAGCATAAAGTGTAA AGCCTGGGGGTGCCTAATGCGGCCGCCATAGTGACTGGATATGTTGTGTT TTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATT GATATTTATATCATTTTACGTTTCTCGTTCAACTTTATTATACATAGTTG ATAATTCACTGGCCGTCGTTTTACATCGATGATGATCCAGACATGATAAG ATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAAT GCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATA AGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCA GGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACA AATGTGGTATGGCTGATTATGATCCTCTAGATCAGATCTGCGAAGATACG GCCACGGGTGCTCTTGATCCTGTGGCTGATTTTGGACTGTGCTGCTCGCA GCTGCTGATGAATCACATACTTCCTCCATTTTCTTCCACTGATTGACTGT TATAATTTCCCTAATTTCCAGGTCAAGGTGCTGTGCATTGTGGTAATAGA TGTGACATGACGTCACTTCCAAAGGACCAATGAACATGTCTGACCAATTT CATATAATGTGAAAACGATTTTCATAGGCAGAATAAATAACATTTAAATT AAACTGGGCATCAGCGCAATTCAATTGGTTTGGTAATAGCAAGGGAAAAT AGAATGAAGTGATCTCCAAAAAATAAGTACTTTTTGACTGTAAATAAAAT TGTAAGGAGTAAAAAGTACTTTTTTTTCTAAAAAAATGTAATTAAGTAAA AGTAAAAGTATTGATTTTTAATTGTACTCAAGTAAAGTAAAAATCCCCAA AAATAATACTTAAGTACAGTAATCAAGTAAAATTACTCAAGTACTTTACA CCTCTGGTTCTTGACCCCCTACCTTCAGCAAGCCCAGCAGATCCACTAGT TCTAGAGCGGCCGCCACCGCGGTGGAGCTCCAGCTTTTGTTCCCTTTAGT GAGGGTTAATTGCGCGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTG TGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCAT AAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTG CGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTG CATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCG CTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGC GGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAA TCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGC CAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCC CCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAAC CCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGT GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTC TCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTC AGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCC CGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCA ACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGG ATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTG GCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGC TGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAA CAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTAC GCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGT CTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGA TTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTT TAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAAT GCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCC ATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTT ACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGG CTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGA AGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCG GGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTG CCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCA TTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTT GTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTA AGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCT CTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTC AACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCC CGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTG CTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACC GCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTT CAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGG CAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACT CATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTC TCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGG GTTCCGCGCACATTTCCCCGAAAAGTG >pBSII_SK+_3’_of_SpeI ACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTCCAGCTTTTGTTCCC TTTAGTGAGGGTTAATTGCGCGCTTGGCGTAATCATGGTCATAGCTGTTT CCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGG AAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACAT TAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGC CAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTAT TGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTC GGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCC ACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCA AAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGG CGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTC CCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCG CCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGG TATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGA ACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGT AACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAA GTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCG CTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCC GGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCA GATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTA CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTC ATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATG AAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTT ACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGT TCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGA GGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCT CACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAG CGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTG TTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACG TTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATG GCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCC CATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCA GAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCAT AATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGA GTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCT CTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTA AAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGAT CTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACT GATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACA GGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTG AATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTT ATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAA ATAGGGGTTCCGCGCACATTTCCCCGAAAAGTG >amp ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATT TTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATG CTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGAT GAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACG CCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGT AAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCA ACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCT GAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAA TGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACC ACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTG GAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAC TATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTA AGCATTGG >pUC_ori TGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCT GGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGAC GCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCG TTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCT TACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC ATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAG CTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATC CGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCAC TGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGT GCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGAC AGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAG TTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTT TTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGA TCCTTTGATCTTTTCTAC >M13R GGAAACAGCTATGACCATGA >T3_primer AATTAACCCTCACTAAAGGG >Tyro_frag_ex5 GATCTGCTGGGCTTGCTGAAGGTAGGGGGTCAAGAAC >5'_TIR CAGAGGTGTAAAGTACTTG >517_bp_of_Tol2_5'_end CAGAGGTGTAAAGTACTTGAGTAATTTTACTTGATTACTGTACTTAAGTA TTATTTTTGGGGATTTTTACTTTACTTGAGTACAATTAAAAATCAATACT TTTACTTTTACTTAATTACATTTTTTTAGAAAAAAAAGTACTTTTTACTC CTTACAATTTTATTTACAGTCAAAAAGTACTTATTTTTTGGAGATCACTT CATTCTATTTTCCCTTGCTATTACCAAACCAATTGAATTGCGCTGATGCC CAGTTTAATTTAAATGTTATTTATTCTGCCTATGAAAATCGTTTTCACAT TATATGAAATTGGTCAGACATGTTCATTGGTCCTTTGGAAGTGACGTCAT GTCACATCTATTACCACAATGCACAGCACCTTGACCTGGAAATTAGGGAA ATTATAACAGTCAATCAGTGGAAGAAAATGGAGGAAGTATGTGATTCATC AGCAGCTGCGAGCAGCACAGTCCAAAATCAGCCACAGGATCAAGAGCACC CGTGGCCGTATCTTCGC >Tol2_exon1,_bp1-335_(of_461) ATTTTTTGGAGATCACTTCATTCTATTTTCCCTTGCTATTACCAAACCAA TTGAATTGCGCTGATGCCCAGTTTAATTTAAATGTTATTTATTCTGCCTA TGAAAATCGTTTTCACATTATATGAAATTGGTCAGACATGTTCATTGGTC CTTTGGAAGTGACGTCATGTCACATCTATTACCACAATGCACAGCACCTT GACCTGGAAATTAGGGAAATTATAACAGTCAATCAGTGGAAGAAAATGGA GGAAGTATGTGATTCATCAGCAGCTGCGAGCAGCACAGTCCAAAATCAGC CACAGGATCAAGAGCACCCGTGGCCGTATCTTCGC >SV40_late_polyA GATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAG AATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTT TATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGC ATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAG CAAGTAAAACCTCTACAAATGTGGTATGGCTGATTAT >M13_-20 GTAAAACGACGGCCAGTG >attR3 CCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTT TATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCT CGTTCAACTTTATTATACATAGTTG >attB3/L3/R3_shared CAACTTTATTATACA >cmR TTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCA TTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCC AGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAA AACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGG TGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAAC CCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGA ATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCG ATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACA CTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGG ATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACT TGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGA ACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATG TTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTT TTTTCTCCAT >ccdB TTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGC GGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTG GCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCAC CGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCA GGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACA TCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAA CTGCAT >ccdB_from_pDEST_R4-R3_(1_mutation) ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTT TGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGA TCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCCCCCGTGAACTT TACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGA TATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCA GCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGA ATATAA >attR4 CAACTTTGTATAGAAAAGTTGAACGAGAAACGTAAAATGATATAAATATC AATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAA AACACAACATATCCAGTCACTATGG >attB4/L4/R4_shared CAACTTTGTATAGAAAAGTTG >M13R GGAAACAGCTATGACCATGA >Tol2_exon4_136-482 ATCTGGCCTGTGTTTCAGACACCAGGGAGTCTCTGCTCACGTTTCCTGCT ATTTGCAGCCTCTCTATCAAGACTAATACACCTCTTCCCGCATCGGCTGC CTGTGAGAGGCTTTTCAGCACTGCAGGATTGCTTTTCAGCCCCAAAAGAG CTAGGCTTGACACTAACAATTTTGAGAATCAGCTTCTACTGAAGTTAAAT CTGAGGTTTTACAACTTTGAGTAGCGTGTACTGGCATTAGATTGTCTGTC TTATAGTTTGATAATTAAATACAAACAGTTCTAAAGCAGGATAAAACCTT GTATGCATTTCATTTAATGTTTTTTGAGATTAAAAGCTTAAACAAGA >536_bp_of_Tol2_3'_end ATCTGGCCTGTGTTTCAGACACCAGGGAGTCTCTGCTCACGTTTCCTGCT ATTTGCAGCCTCTCTATCAAGACTAATACACCTCTTCCCGCATCGGCTGC CTGTGAGAGGCTTTTCAGCACTGCAGGATTGCTTTTCAGCCCCAAAAGAG CTAGGCTTGACACTAACAATTTTGAGAATCAGCTTCTACTGAAGTTAAAT CTGAGGTTTTACAACTTTGAGTAGCGTGTACTGGCATTAGATTGTCTGTC TTATAGTTTGATAATTAAATACAAACAGTTCTAAAGCAGGATAAAACCTT GTATGCATTTCATTTAATGTTTTTTGAGATTAAAAGCTTAAACAAGAATC TCTAGTTTTCTTTCTTGCTTTTACTTTTACTTCCTTAATACTCAAGTACA ATTTTAATGGAGTACTTTTTTACTTTTACTCAAGTAAGATTCTAGCCAGA TACTTTTACTTTTAATTGAGTAAAATTTTCCCTAAGTACTTGTACTTTCA CTTGAGTAAAATTTTTGAGTACTTTTTACACCTCTG >3'_TIR TACTTTTTACACCTCTG >tyrosinase_genomic_from_AB010101 GCTGGCTAAGAACTCATCAGCCTCCCCGGTCCATCTACCCACGTACCAAT GCACCAATTGGCCACAATGACGGCTACTACATGGTGCCATTCCTTCCTCT TTATAGGAATGGAGACTACCTCCTGTCCAACAAGGCTCTTGGATACGAGT ACGCCTACCTGTTGGACCCAGGTCATTGCACAACACCAGAAATGCCCTCT GATCTGCAAAAGACGTGAATATCTGTTCAGACACCCATATCCACTCTGTT CCACACAGGTCAGAGGTTTGTCCAGGAGTTCTTGAC >Tyro_frag_ex4-in4-ex5 CAGGTCATTGCACAACACCAGAAATGCCCTCTGATCTGCAAAAGACGTGA ATATCTGTTCAGACACCCATATCCACTCTGTTCCACACAGG >f1_(+)_ori_fragment GTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCC ACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAA >pBSII_SK+_5’_of_DraIII CCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTT TGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCC TTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTT GGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGA AAAACCGTCTATCAGGGCGATGGCCCAC