From Tol2Kit
(PDestTol2pA sequence moved to PDestTol2pA2 sequence: typo in original name) |
|||
Line 1: | Line 1: | ||
− | + | <pre> | |
+ | >pDest-Tol2-pA | ||
+ | CCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTT | ||
+ | TGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCC | ||
+ | TTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTT | ||
+ | GGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGA | ||
+ | AAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATC | ||
+ | AAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAG | ||
+ | GGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGA | ||
+ | AAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGT | ||
+ | AGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGC | ||
+ | TACAGGGCGCGTCCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGG | ||
+ | CGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATG | ||
+ | TGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGAC | ||
+ | GTTGTAAAACGACGGCCAGTGAGCGCGCGTAATACGACTCACTATAGGGC | ||
+ | GAATTGGGTACCAAATAGTAGGAATTACCCACCTGTACAAGTGCTGAAAA | ||
+ | CTTGGATGAATAAGCCCGTTTGCCATTTTCTACTGCTATTTTAAATCTTT | ||
+ | TCTGTATTTGTCTGCATTTGTCTTTACCCTTGAATAAATGTTTTAGTTGT | ||
+ | TTTTTTTCAATTATGACTGTGTTTAACAGACATTATTACAATAATATGTA | ||
+ | ATAGTAGTACACACTATTATTATGTAATAGTACTTGTTGACTGTATTTGA | ||
+ | GAACTGGACCGAGTGAGTGTTACGTCACCCATTCAAAATGACTTACTTCT | ||
+ | GGCTCCAACAAAATGAAGTTAATTCAGTTGCCATTTTTCACTGTATGGAC | ||
+ | ATCGCCGTGTTGGAGCTAGACGTCGCCAATAAGCAAGAAAGAGCCGAGAT | ||
+ | GCGTCGAGTCTGAGTCACCGTTCCTATGGCAACCCCTCTAACCAATCAGA | ||
+ | AGTAAGCTTGTTGGAAGTCCACAGCCTACCACTTGAAAGCGGGCTGCACA | ||
+ | AAATCTGTCAAACGTTTTGAACGTTGGATGTGAGAGCACATACTTTTATT | ||
+ | AAGGCATCTGGTTGGTCAGTTTATAATATCAACAACTTGGGCTACAGAAA | ||
+ | AGAAAAGTTATTACAGAAATTATGATTAACAAGTACATGTTAAATAAAGA | ||
+ | TTTTAATATGAATGCCACCACTGGAGCATTCATGCCATTTGGAGCTTCTT | ||
+ | CCTGTTTGGATCACTAGAAGGAGGAGGTCACTCATTACAGTTCTCATATA | ||
+ | CAGTCGTTGGTTGGTTGGTTGGTTGGTTGGTTGGTAGATTGATTGATTGA | ||
+ | TTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATT | ||
+ | GATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGGTAGTCAAA | ||
+ | ATAAGAAATAATTTCCACAGATTCATTACAGAAATGATTAAATGCATACA | ||
+ | TAAAAAACTGGGGGGGGGGGGATACAACAACACACTTAAGTCACATTTGC | ||
+ | CTACGTAAAGAAAAGTAAAGAAAATCAATAGCTATATTTTACATCTCCTT | ||
+ | TTTTTGCTGTCTTTTAATTAGCCTTGTTTTGCTGTTATCTTGATTGAAAC | ||
+ | TGTAACTTCTTCACCTGCTTCTTTTCTTTGTAGGTTTTGCCAGCCCAGAA | ||
+ | ACAGGCATGGCTGTGCAAGGCCAGAGCACCATGCACAATGCGCTGCATGT | ||
+ | CTTCATGAACGGCTCAATGTCCTCAGTCCAGGGCTCAGCCAACGACCCCA | ||
+ | TCTTCCTCCTTCACCATGCTTTCATTGACAGGTAACAAACACGTCATGAC | ||
+ | ATTAGACTGCACAGTTTTTGACAAAGTTCATACAATCTGTTGTTTATAGC | ||
+ | TGCTACAATTAGTGAAGTTTGTGAATGTACTTGGATGAGCAGCGAAAGAT | ||
+ | CAATTGAGATCAATTGTTAGAGTTTGGTTGCCCTGCAGAGCAAAGAACAA | ||
+ | AAAATAATCTGGTGGCTTTACTGCGTGAGGTTATTATTGGTGGAATAGAA | ||
+ | ACACAAAACATAATTGCATTTATTTGTTTAATTTTTTATCTTATCTTAAC | ||
+ | TTTCATCTTGCATATTTGTTTCTTACATCATTTCTAGCATCTTTGAGCGC | ||
+ | TGGCTAAGAACTCATCAGCCTCCCCGGTCCATCTACCCACGTACCAATGC | ||
+ | ACCAATTGGCCACAATGACGGCTACTACATGGTGCCATTCCTTCCTCTTT | ||
+ | ATAGGAATGGAGACTACCTCCTGTCCAACAAGGCTCTTGGATACGAGTAC | ||
+ | GCCTACCTGTTGGACCCAGGTCATTGCACAACACCAGAAATGCCCTCTGA | ||
+ | TCTGCAAAAGACGTGAATATCTGTTCAGACACCCATATCCACTCTGTTCC | ||
+ | ACACAGGTCAGAGGTTTGTCCAGGAGTTCTTGACAGAGGTGTAAAAAGTA | ||
+ | CTCAAAAATTTTACTCAAGTGAAAGTACAAGTACTTAGGGAAAATTTTAC | ||
+ | TCAATTAAAAGTAAAAGTATCTGGCTAGAATCTTACTTGAGTAAAAGTAA | ||
+ | AAAAGTACTCCATTAAAATTGTACTTGAGTATTAAGGAAGTAAAAGTAAA | ||
+ | AGCAAGAAAGAAAACTAGAGATTCTTGTTTAAGCTTTTAATCTCAAAAAA | ||
+ | CATTAAATGAAATGCATACAAGGTTTTATCCTGCTTTAGAACTGTTTGTA | ||
+ | TTTAATTATCAAACTATAAGACAGACAATCTAATGCCAGTACACGCTACT | ||
+ | CAAAGTTGTAAAACCTCAGATTTAACTTCAGTAGAAGCTGATTCTCAAAA | ||
+ | TTGTTAGTGTCAAGCCTAGCTCTTTTGGGGCTGAAAAGCAATCCTGCAGT | ||
+ | GCTGAAAAGCCTCTCACAGGCAGCCGATGCGGGAAGAGGTGTATTAGTCT | ||
+ | TGATAGAGAGGCTGCAAATAGCAGGAAACGTGAGCAGAGACTCCCTGGTG | ||
+ | TCTGAAACACAGGCCAGATGGGCCCTCGAGCAGGAAACAGCTATGACCAT | ||
+ | GATTACGCCAAGCTATCAACTTTGTATAGAAAAGTTGAACGAGAAACGTA | ||
+ | AAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGA | ||
+ | CTACATAATACTGTAAAACACAACATATCCAGTCACTATGGTCGACCTGC | ||
+ | AGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCA | ||
+ | GGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACT | ||
+ | TCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCAT | ||
+ | GCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGGGA | ||
+ | CTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGC | ||
+ | CCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACG | ||
+ | GCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGCCCCTG | ||
+ | TTCTCGTCGGCAAAGGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGC | ||
+ | CATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGG | ||
+ | CTTCATTCTGCATGGTTGTGCTTACCGAACCGGAGATATTGACATCATAT | ||
+ | ATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGC | ||
+ | TGCTTCATAGCATACCTCTTTTTGACATACTTCGGGTATACATATCAGTA | ||
+ | TATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCT | ||
+ | GGCTTTTAGTAAGCCGGATCCTCTAGATTACGCCCCGCCCTGCCACTCAT | ||
+ | CGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCA | ||
+ | CAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCC | ||
+ | TTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCA | ||
+ | TATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCT | ||
+ | GAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTT | ||
+ | TTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGA | ||
+ | AATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCA | ||
+ | TGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACC | ||
+ | GTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAA | ||
+ | GAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTC | ||
+ | TTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTG | ||
+ | AGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATA | ||
+ | TATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTA | ||
+ | GCTCCTGAAAATCTCGACGGATCCTAACTCAAAATCCACACATTATACGA | ||
+ | GCCGGAAGCATAAAGTGTAAAGCCTGGGGGTGCCTAATGCGGCCGCCATA | ||
+ | GTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGC | ||
+ | AAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTC | ||
+ | AACTTTATTATACATAGTTGATAATTCACTGGCCGTCGTTTTACATCGAT | ||
+ | GATGATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAAC | ||
+ | TAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTG | ||
+ | CTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAAT | ||
+ | TGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTA | ||
+ | AAGCAAGTAAAACCTCTACAAATGTGGTATGGCTGATTATGATCCTCTAG | ||
+ | ATCAGATCTGCGAAGATACGGCCACGGGTGCTCTTGATCCTGTGGCTGAT | ||
+ | TTTGGACTGTGCTGCTCGCAGCTGCTGATGAATCACATACTTCCTCCATT | ||
+ | TTCTTCCACTGATTGACTGTTATAATTTCCCTAATTTCCAGGTCAAGGTG | ||
+ | CTGTGCATTGTGGTAATAGATGTGACATGACGTCACTTCCAAAGGACCAA | ||
+ | TGAACATGTCTGACCAATTTCATATAATGTGAAAACGATTTTCATAGGCA | ||
+ | GAATAAATAACATTTAAATTAAACTGGGCATCAGCGCAATTCAATTGGTT | ||
+ | TGGTAATAGCAAGGGAAAATAGAATGAAGTGATCTCCAAAAAATAAGTAC | ||
+ | TTTTTGACTGTAAATAAAATTGTAAGGAGTAAAAAGTACTTTTTTTTCTA | ||
+ | AAAAAATGTAATTAAGTAAAAGTAAAAGTATTGATTTTTAATTGTACTCA | ||
+ | AGTAAAGTAAAAATCCCCAAAAATAATACTTAAGTACAGTAATCAAGTAA | ||
+ | AATTACTCAAGTACTTTACACCTCTGGTTCTTGACCCCCTACCTTCAGCA | ||
+ | AGCCCAGCAGATCCACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTC | ||
+ | CAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGCGCTTGGCGTAATCAT | ||
+ | GGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACAC | ||
+ | AACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGT | ||
+ | GAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGG | ||
+ | GAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGA | ||
+ | GGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCT | ||
+ | GCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGG | ||
+ | TAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGA | ||
+ | GCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGC | ||
+ | GTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCT | ||
+ | CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTT | ||
+ | CCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTAC | ||
+ | CGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATA | ||
+ | GCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTG | ||
+ | GGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGG | ||
+ | TAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGG | ||
+ | CAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCT | ||
+ | ACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGT | ||
+ | ATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTG | ||
+ | GTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTT | ||
+ | GTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCC | ||
+ | TTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTT | ||
+ | AAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTT | ||
+ | TTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAAC | ||
+ | TTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGA | ||
+ | TCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATA | ||
+ | ACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACC | ||
+ | GCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAG | ||
+ | CCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATC | ||
+ | CAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAA | ||
+ | TAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCT | ||
+ | CGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGA | ||
+ | GTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCC | ||
+ | TCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTA | ||
+ | TGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTT | ||
+ | TCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCG | ||
+ | GCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCAC | ||
+ | ATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGA | ||
+ | AAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCAC | ||
+ | TCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTG | ||
+ | GGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCG | ||
+ | ACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAG | ||
+ | CATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTT | ||
+ | AGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTG | ||
+ | >pBSII_SK+_3’_of_SpeI | ||
+ | ACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTCCAGCTTTTGTTCCC | ||
+ | TTTAGTGAGGGTTAATTGCGCGCTTGGCGTAATCATGGTCATAGCTGTTT | ||
+ | CCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGG | ||
+ | AAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACAT | ||
+ | TAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGC | ||
+ | CAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTAT | ||
+ | TGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTC | ||
+ | GGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCC | ||
+ | ACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCA | ||
+ | AAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC | ||
+ | TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGG | ||
+ | CGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTC | ||
+ | CCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCG | ||
+ | CCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGG | ||
+ | TATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGA | ||
+ | ACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG | ||
+ | AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGT | ||
+ | AACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAA | ||
+ | GTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCG | ||
+ | CTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCC | ||
+ | GGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCA | ||
+ | GATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTA | ||
+ | CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTC | ||
+ | ATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATG | ||
+ | AAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTT | ||
+ | ACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGT | ||
+ | TCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGA | ||
+ | GGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCT | ||
+ | CACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAG | ||
+ | CGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTG | ||
+ | TTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACG | ||
+ | TTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATG | ||
+ | GCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCC | ||
+ | CATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCA | ||
+ | GAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCAT | ||
+ | AATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGA | ||
+ | GTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCT | ||
+ | CTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTA | ||
+ | AAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGAT | ||
+ | CTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACT | ||
+ | GATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACA | ||
+ | GGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTG | ||
+ | AATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTT | ||
+ | ATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAA | ||
+ | ATAGGGGTTCCGCGCACATTTCCCCGAAAAGTG | ||
+ | >amp | ||
+ | ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATT | ||
+ | TTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATG | ||
+ | CTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC | ||
+ | AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGAT | ||
+ | GAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACG | ||
+ | CCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG | ||
+ | GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGT | ||
+ | AAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCA | ||
+ | ACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG | ||
+ | CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCT | ||
+ | GAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAA | ||
+ | TGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT | ||
+ | TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACC | ||
+ | ACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTG | ||
+ | GAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT | ||
+ | GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAC | ||
+ | TATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTA | ||
+ | AGCATTGG | ||
+ | >pUC_ori | ||
+ | TGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCT | ||
+ | GGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGAC | ||
+ | GCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCG | ||
+ | TTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCT | ||
+ | TACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC | ||
+ | ATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAG | ||
+ | CTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATC | ||
+ | CGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCAC | ||
+ | TGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGT | ||
+ | GCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGAC | ||
+ | AGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAG | ||
+ | TTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTT | ||
+ | TTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGA | ||
+ | TCCTTTGATCTTTTCTAC | ||
+ | >M13R | ||
+ | GGAAACAGCTATGACCATGA | ||
+ | >T3_primer | ||
+ | AATTAACCCTCACTAAAGGG | ||
+ | >Tyro_frag_ex5 | ||
+ | GATCTGCTGGGCTTGCTGAAGGTAGGGGGTCAAGAAC | ||
+ | >5'_TIR | ||
+ | CAGAGGTGTAAAGTACTTG | ||
+ | >517_bp_of_Tol2_5'_end | ||
+ | CAGAGGTGTAAAGTACTTGAGTAATTTTACTTGATTACTGTACTTAAGTA | ||
+ | TTATTTTTGGGGATTTTTACTTTACTTGAGTACAATTAAAAATCAATACT | ||
+ | TTTACTTTTACTTAATTACATTTTTTTAGAAAAAAAAGTACTTTTTACTC | ||
+ | CTTACAATTTTATTTACAGTCAAAAAGTACTTATTTTTTGGAGATCACTT | ||
+ | CATTCTATTTTCCCTTGCTATTACCAAACCAATTGAATTGCGCTGATGCC | ||
+ | CAGTTTAATTTAAATGTTATTTATTCTGCCTATGAAAATCGTTTTCACAT | ||
+ | TATATGAAATTGGTCAGACATGTTCATTGGTCCTTTGGAAGTGACGTCAT | ||
+ | GTCACATCTATTACCACAATGCACAGCACCTTGACCTGGAAATTAGGGAA | ||
+ | ATTATAACAGTCAATCAGTGGAAGAAAATGGAGGAAGTATGTGATTCATC | ||
+ | AGCAGCTGCGAGCAGCACAGTCCAAAATCAGCCACAGGATCAAGAGCACC | ||
+ | CGTGGCCGTATCTTCGC | ||
+ | >Tol2_exon1,_bp1-335_(of_461) | ||
+ | ATTTTTTGGAGATCACTTCATTCTATTTTCCCTTGCTATTACCAAACCAA | ||
+ | TTGAATTGCGCTGATGCCCAGTTTAATTTAAATGTTATTTATTCTGCCTA | ||
+ | TGAAAATCGTTTTCACATTATATGAAATTGGTCAGACATGTTCATTGGTC | ||
+ | CTTTGGAAGTGACGTCATGTCACATCTATTACCACAATGCACAGCACCTT | ||
+ | GACCTGGAAATTAGGGAAATTATAACAGTCAATCAGTGGAAGAAAATGGA | ||
+ | GGAAGTATGTGATTCATCAGCAGCTGCGAGCAGCACAGTCCAAAATCAGC | ||
+ | CACAGGATCAAGAGCACCCGTGGCCGTATCTTCGC | ||
+ | >SV40_late_polyA | ||
+ | GATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAG | ||
+ | AATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTT | ||
+ | TATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGC | ||
+ | ATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAG | ||
+ | CAAGTAAAACCTCTACAAATGTGGTATGGCTGATTAT | ||
+ | >M13_-20 | ||
+ | GTAAAACGACGGCCAGTG | ||
+ | >attR3 | ||
+ | CCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTT | ||
+ | TATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCT | ||
+ | CGTTCAACTTTATTATACATAGTTG | ||
+ | >attB3/L3/R3_shared | ||
+ | CAACTTTATTATACA | ||
+ | >cmR | ||
+ | TTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCA | ||
+ | TTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCC | ||
+ | AGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAA | ||
+ | AACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGG | ||
+ | TGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAAC | ||
+ | CCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGA | ||
+ | ATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCG | ||
+ | ATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACA | ||
+ | CTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGG | ||
+ | ATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACT | ||
+ | TGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGA | ||
+ | ACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATG | ||
+ | TTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTT | ||
+ | TTTTCTCCAT | ||
+ | >ccdB_from_pDEST_R4-R3_(1_mutation) | ||
+ | ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTT | ||
+ | TGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGA | ||
+ | TCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCCCCCGTGAACTT | ||
+ | TACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGA | ||
+ | TATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCA | ||
+ | GCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGA | ||
+ | ATATAA | ||
+ | >attR4 | ||
+ | CAACTTTGTATAGAAAAGTTGAACGAGAAACGTAAAATGATATAAATATC | ||
+ | AATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAA | ||
+ | AACACAACATATCCAGTCACTATGG | ||
+ | >attB4/L4/R4_shared | ||
+ | CAACTTTGTATAGAAAAGTTG | ||
+ | >M13R | ||
+ | GGAAACAGCTATGACCATGA | ||
+ | >Tol2_exon4_136-482 | ||
+ | ATCTGGCCTGTGTTTCAGACACCAGGGAGTCTCTGCTCACGTTTCCTGCT | ||
+ | ATTTGCAGCCTCTCTATCAAGACTAATACACCTCTTCCCGCATCGGCTGC | ||
+ | CTGTGAGAGGCTTTTCAGCACTGCAGGATTGCTTTTCAGCCCCAAAAGAG | ||
+ | CTAGGCTTGACACTAACAATTTTGAGAATCAGCTTCTACTGAAGTTAAAT | ||
+ | CTGAGGTTTTACAACTTTGAGTAGCGTGTACTGGCATTAGATTGTCTGTC | ||
+ | TTATAGTTTGATAATTAAATACAAACAGTTCTAAAGCAGGATAAAACCTT | ||
+ | GTATGCATTTCATTTAATGTTTTTTGAGATTAAAAGCTTAAACAAGA | ||
+ | >536_bp_of_Tol2_3'_end | ||
+ | ATCTGGCCTGTGTTTCAGACACCAGGGAGTCTCTGCTCACGTTTCCTGCT | ||
+ | ATTTGCAGCCTCTCTATCAAGACTAATACACCTCTTCCCGCATCGGCTGC | ||
+ | CTGTGAGAGGCTTTTCAGCACTGCAGGATTGCTTTTCAGCCCCAAAAGAG | ||
+ | CTAGGCTTGACACTAACAATTTTGAGAATCAGCTTCTACTGAAGTTAAAT | ||
+ | CTGAGGTTTTACAACTTTGAGTAGCGTGTACTGGCATTAGATTGTCTGTC | ||
+ | TTATAGTTTGATAATTAAATACAAACAGTTCTAAAGCAGGATAAAACCTT | ||
+ | GTATGCATTTCATTTAATGTTTTTTGAGATTAAAAGCTTAAACAAGAATC | ||
+ | TCTAGTTTTCTTTCTTGCTTTTACTTTTACTTCCTTAATACTCAAGTACA | ||
+ | ATTTTAATGGAGTACTTTTTTACTTTTACTCAAGTAAGATTCTAGCCAGA | ||
+ | TACTTTTACTTTTAATTGAGTAAAATTTTCCCTAAGTACTTGTACTTTCA | ||
+ | CTTGAGTAAAATTTTTGAGTACTTTTTACACCTCTG | ||
+ | >Tol2_primer | ||
+ | AGGCTGCAAATAGCAGGAAA | ||
+ | >3'_TIR | ||
+ | TACTTTTTACACCTCTG | ||
+ | >tyrosinase_genomic_from_AB010101 | ||
+ | GGTACCAAATAGTAGGAATTACCCACCTGTACAAGTGCTGAAAACTTGGA | ||
+ | TGAATAAGCCCGTTTGCCATTTTCTACTGCTATTTTAAATCTTTTCTGTA | ||
+ | TTTGTCTGCATTTGTCTTTACCCTTGAATAAATGTTTTAGTTGTTTTTTT | ||
+ | TCAATTATGACTGTGTTTAACAGACATTATTACAATAATATGTAATAGTA | ||
+ | GTACACACTATTATTATGTAATAGTACTTGTTGACTGTATTTGAGAACTG | ||
+ | GACCGAGTGAGTGTTACGTCACCCATTCAAAATGACTTACTTCTGGCTCC | ||
+ | AACAAAATGAAGTTAATTCAGTTGCCATTTTTCACTGTATGGACATCGCC | ||
+ | GTGTTGGAGCTAGACGTCGCCAATAAGCAAGAAAGAGCCGAGATGCGTCG | ||
+ | AGTCTGAGTCACCGTTCCTATGGCAACCCCTCTAACCAATCAGAAGTAAG | ||
+ | CTTGTTGGAAGTCCACAGCCTACCACTTGAAAGCGGGCTGCACAAAATCT | ||
+ | GTCAAACGTTTTGAACGTTGGATGTGAGAGCACATACTTTTATTAAGGCA | ||
+ | TCTGGTTGGTCAGTTTATAATATCAACAACTTGGGCTACAGAAAAGAAAA | ||
+ | GTTATTACAGAAATTATGATTAACAAGTACATGTTAAATAAAGATTTTAA | ||
+ | TATGAATGCCACCACTGGAGCATTCATGCCATTTGGAGCTTCTTCCTGTT | ||
+ | TGGATCACTAGAAGGAGGAGGTCACTCATTACAGTTCTCATATACAGTCG | ||
+ | TTGGTTGGTTGGTTGGTTGGTTGGTTGGTAGATTGATTGATTGATTGATT | ||
+ | GATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGA | ||
+ | TTGATTGATTGATTGATTGATTGATTGATTGATTGGTAGTCAAAATAAGA | ||
+ | AATAATTTCCACAGATTCATTACAGAAATGATTAAATGCATACATAAAAA | ||
+ | ACTGGGGGGGGGGGGATACAACAACACACTTAAGTCACATTTGCCTACGT | ||
+ | AAAGAAAAGTAAAGAAAATCAATAGCTATATTTTACATCTCCTTTTTTTG | ||
+ | CTGTCTTTTAATTAGCCTTGTTTTGCTGTTATCTTGATTGAAACTGTAAC | ||
+ | TTCTTCACCTGCTTCTTTTCTTTGTAGGTTTTGCCAGCCCAGAAACAGGC | ||
+ | ATGGCTGTGCAAGGCCAGAGCACCATGCACAATGCGCTGCATGTCTTCAT | ||
+ | GAACGGCTCAATGTCCTCAGTCCAGGGCTCAGCCAACGACCCCATCTTCC | ||
+ | TCCTTCACCATGCTTTCATTGACAGGTAACAAACACGTCATGACATTAGA | ||
+ | CTGCACAGTTTTTGACAAAGTTCATACAATCTGTTGTTTATAGCTGCTAC | ||
+ | AATTAGTGAAGTTTGTGAATGTACTTGGATGAGCAGCGAAAGATCAATTG | ||
+ | AGATCAATTGTTAGAGTTTGGTTGCCCTGCAGAGCAAAGAACAAAAAATA | ||
+ | ATCTGGTGGCTTTACTGCGTGAGGTTATTATTGGTGGAATAGAAACACAA | ||
+ | AACATAATTGCATTTATTTGTTTAATTTTTTATCTTATCTTAACTTTCAT | ||
+ | CTTGCATATTTGTTTCTTACATCATTTCTAGCATCTTTGAGCGCTGGCTA | ||
+ | AGAACTCATCAGCCTCCCCGGTCCATCTACCCACGTACCAATGCACCAAT | ||
+ | TGGCCACAATGACGGCTACTACATGGTGCCATTCCTTCCTCTTTATAGGA | ||
+ | ATGGAGACTACCTCCTGTCCAACAAGGCTCTTGGATACGAGTACGCCTAC | ||
+ | CTGTTGGACCCAGGTCATTGCACAACACCAGAAATGCCCTCTGATCTGCA | ||
+ | AAAGACGTGAATATCTGTTCAGACACCCATATCCACTCTGTTCCACACAG | ||
+ | GTCAGAGGTTTGTCCAGGAGTTCTTGAC | ||
+ | >Tyro_frag_ex4-in4-ex5 | ||
+ | CAGGTCATTGCACAACACCAGAAATGCCCTCTGATCTGCAAAAGACGTGA | ||
+ | ATATCTGTTCAGACACCCATATCCACTCTGTTCCACACAGG | ||
+ | >pBSII_SK+_5’_of_KpnI | ||
+ | CCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTT | ||
+ | TGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCC | ||
+ | TTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTT | ||
+ | GGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGA | ||
+ | AAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATC | ||
+ | AAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAG | ||
+ | GGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGA | ||
+ | AAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGT | ||
+ | AGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGC | ||
+ | TACAGGGCGCGTCCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGG | ||
+ | CGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATG | ||
+ | TGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGAC | ||
+ | GTTGTAAAACGACGGCCAGTGAGCGCGCGTAATACGACTCACTATAGGGC | ||
+ | GAATTGGGTACC | ||
+ | >T7_primer | ||
+ | GTAATACGACTCACTATAGGGCG | ||
+ | >M13_-20 | ||
+ | GTAAAACGACGGCCAGTG | ||
+ | >f1_(+)_ori | ||
+ | GCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACT | ||
+ | TGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCG | ||
+ | CCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTA | ||
+ | GGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTA | ||
+ | GGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCC | ||
+ | CTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACT | ||
+ | GGAACAA | ||
+ | </pre> |
Revision as of 05:01, 18 April 2007
>pDest-Tol2-pA CCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTT TGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCC TTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTT GGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGA AAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATC AAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAG GGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGA AAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGT AGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGC TACAGGGCGCGTCCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGG CGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATG TGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGAC GTTGTAAAACGACGGCCAGTGAGCGCGCGTAATACGACTCACTATAGGGC GAATTGGGTACCAAATAGTAGGAATTACCCACCTGTACAAGTGCTGAAAA CTTGGATGAATAAGCCCGTTTGCCATTTTCTACTGCTATTTTAAATCTTT TCTGTATTTGTCTGCATTTGTCTTTACCCTTGAATAAATGTTTTAGTTGT TTTTTTTCAATTATGACTGTGTTTAACAGACATTATTACAATAATATGTA ATAGTAGTACACACTATTATTATGTAATAGTACTTGTTGACTGTATTTGA GAACTGGACCGAGTGAGTGTTACGTCACCCATTCAAAATGACTTACTTCT GGCTCCAACAAAATGAAGTTAATTCAGTTGCCATTTTTCACTGTATGGAC ATCGCCGTGTTGGAGCTAGACGTCGCCAATAAGCAAGAAAGAGCCGAGAT GCGTCGAGTCTGAGTCACCGTTCCTATGGCAACCCCTCTAACCAATCAGA AGTAAGCTTGTTGGAAGTCCACAGCCTACCACTTGAAAGCGGGCTGCACA AAATCTGTCAAACGTTTTGAACGTTGGATGTGAGAGCACATACTTTTATT AAGGCATCTGGTTGGTCAGTTTATAATATCAACAACTTGGGCTACAGAAA AGAAAAGTTATTACAGAAATTATGATTAACAAGTACATGTTAAATAAAGA TTTTAATATGAATGCCACCACTGGAGCATTCATGCCATTTGGAGCTTCTT CCTGTTTGGATCACTAGAAGGAGGAGGTCACTCATTACAGTTCTCATATA CAGTCGTTGGTTGGTTGGTTGGTTGGTTGGTTGGTAGATTGATTGATTGA TTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATT GATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGGTAGTCAAA ATAAGAAATAATTTCCACAGATTCATTACAGAAATGATTAAATGCATACA TAAAAAACTGGGGGGGGGGGGATACAACAACACACTTAAGTCACATTTGC CTACGTAAAGAAAAGTAAAGAAAATCAATAGCTATATTTTACATCTCCTT TTTTTGCTGTCTTTTAATTAGCCTTGTTTTGCTGTTATCTTGATTGAAAC TGTAACTTCTTCACCTGCTTCTTTTCTTTGTAGGTTTTGCCAGCCCAGAA ACAGGCATGGCTGTGCAAGGCCAGAGCACCATGCACAATGCGCTGCATGT CTTCATGAACGGCTCAATGTCCTCAGTCCAGGGCTCAGCCAACGACCCCA TCTTCCTCCTTCACCATGCTTTCATTGACAGGTAACAAACACGTCATGAC ATTAGACTGCACAGTTTTTGACAAAGTTCATACAATCTGTTGTTTATAGC TGCTACAATTAGTGAAGTTTGTGAATGTACTTGGATGAGCAGCGAAAGAT CAATTGAGATCAATTGTTAGAGTTTGGTTGCCCTGCAGAGCAAAGAACAA AAAATAATCTGGTGGCTTTACTGCGTGAGGTTATTATTGGTGGAATAGAA ACACAAAACATAATTGCATTTATTTGTTTAATTTTTTATCTTATCTTAAC TTTCATCTTGCATATTTGTTTCTTACATCATTTCTAGCATCTTTGAGCGC TGGCTAAGAACTCATCAGCCTCCCCGGTCCATCTACCCACGTACCAATGC ACCAATTGGCCACAATGACGGCTACTACATGGTGCCATTCCTTCCTCTTT ATAGGAATGGAGACTACCTCCTGTCCAACAAGGCTCTTGGATACGAGTAC GCCTACCTGTTGGACCCAGGTCATTGCACAACACCAGAAATGCCCTCTGA TCTGCAAAAGACGTGAATATCTGTTCAGACACCCATATCCACTCTGTTCC ACACAGGTCAGAGGTTTGTCCAGGAGTTCTTGACAGAGGTGTAAAAAGTA CTCAAAAATTTTACTCAAGTGAAAGTACAAGTACTTAGGGAAAATTTTAC TCAATTAAAAGTAAAAGTATCTGGCTAGAATCTTACTTGAGTAAAAGTAA AAAAGTACTCCATTAAAATTGTACTTGAGTATTAAGGAAGTAAAAGTAAA AGCAAGAAAGAAAACTAGAGATTCTTGTTTAAGCTTTTAATCTCAAAAAA CATTAAATGAAATGCATACAAGGTTTTATCCTGCTTTAGAACTGTTTGTA TTTAATTATCAAACTATAAGACAGACAATCTAATGCCAGTACACGCTACT CAAAGTTGTAAAACCTCAGATTTAACTTCAGTAGAAGCTGATTCTCAAAA TTGTTAGTGTCAAGCCTAGCTCTTTTGGGGCTGAAAAGCAATCCTGCAGT GCTGAAAAGCCTCTCACAGGCAGCCGATGCGGGAAGAGGTGTATTAGTCT TGATAGAGAGGCTGCAAATAGCAGGAAACGTGAGCAGAGACTCCCTGGTG TCTGAAACACAGGCCAGATGGGCCCTCGAGCAGGAAACAGCTATGACCAT GATTACGCCAAGCTATCAACTTTGTATAGAAAAGTTGAACGAGAAACGTA AAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGA CTACATAATACTGTAAAACACAACATATCCAGTCACTATGGTCGACCTGC AGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCA GGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACT TCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCAT GCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGGGA CTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGC CCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACG GCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGCCCCTG TTCTCGTCGGCAAAGGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGC CATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGG CTTCATTCTGCATGGTTGTGCTTACCGAACCGGAGATATTGACATCATAT ATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGC TGCTTCATAGCATACCTCTTTTTGACATACTTCGGGTATACATATCAGTA TATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCT GGCTTTTAGTAAGCCGGATCCTCTAGATTACGCCCCGCCCTGCCACTCAT CGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCA CAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCC TTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCA TATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCT GAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTT TTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGA AATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCA TGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACC GTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAA GAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTC TTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTG AGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATA TATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTA GCTCCTGAAAATCTCGACGGATCCTAACTCAAAATCCACACATTATACGA GCCGGAAGCATAAAGTGTAAAGCCTGGGGGTGCCTAATGCGGCCGCCATA GTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGC AAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTC AACTTTATTATACATAGTTGATAATTCACTGGCCGTCGTTTTACATCGAT GATGATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAAC TAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTG CTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAAT TGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTA AAGCAAGTAAAACCTCTACAAATGTGGTATGGCTGATTATGATCCTCTAG ATCAGATCTGCGAAGATACGGCCACGGGTGCTCTTGATCCTGTGGCTGAT TTTGGACTGTGCTGCTCGCAGCTGCTGATGAATCACATACTTCCTCCATT TTCTTCCACTGATTGACTGTTATAATTTCCCTAATTTCCAGGTCAAGGTG CTGTGCATTGTGGTAATAGATGTGACATGACGTCACTTCCAAAGGACCAA TGAACATGTCTGACCAATTTCATATAATGTGAAAACGATTTTCATAGGCA GAATAAATAACATTTAAATTAAACTGGGCATCAGCGCAATTCAATTGGTT TGGTAATAGCAAGGGAAAATAGAATGAAGTGATCTCCAAAAAATAAGTAC TTTTTGACTGTAAATAAAATTGTAAGGAGTAAAAAGTACTTTTTTTTCTA AAAAAATGTAATTAAGTAAAAGTAAAAGTATTGATTTTTAATTGTACTCA AGTAAAGTAAAAATCCCCAAAAATAATACTTAAGTACAGTAATCAAGTAA AATTACTCAAGTACTTTACACCTCTGGTTCTTGACCCCCTACCTTCAGCA AGCCCAGCAGATCCACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTC CAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGCGCTTGGCGTAATCAT GGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACAC AACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGT GAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGG GAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGA GGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCT GCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGG TAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGA GCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGC GTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCT CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTT CCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTAC CGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATA GCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTG GGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGG TAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGG CAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCT ACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGT ATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTG GTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTT GTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCC TTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTT AAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTT TTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAAC TTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGA TCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATA ACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACC GCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAG CCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATC CAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAA TAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCT CGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGA GTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCC TCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTA TGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTT TCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCG GCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCAC ATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGA AAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCAC TCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTG GGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCG ACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAG CATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTT AGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTG >pBSII_SK+_3’_of_SpeI ACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTCCAGCTTTTGTTCCC TTTAGTGAGGGTTAATTGCGCGCTTGGCGTAATCATGGTCATAGCTGTTT CCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGG AAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACAT TAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGC CAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTAT TGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTC GGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCC ACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCA AAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGG CGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTC CCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCG CCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGG TATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGA ACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGT AACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAA GTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCG CTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCC GGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCA GATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTA CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTC ATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATG AAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTT ACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGT TCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGA GGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCT CACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAG CGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTG TTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACG TTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATG GCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCC CATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCA GAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCAT AATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGA GTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCT CTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTA AAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGAT CTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACT GATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACA GGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTG AATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTT ATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAA ATAGGGGTTCCGCGCACATTTCCCCGAAAAGTG >amp ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATT TTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATG CTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGAT GAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACG CCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGT AAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCA ACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCT GAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAA TGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACC ACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTG GAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAC TATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTA AGCATTGG >pUC_ori TGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCT GGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGAC GCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCG TTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCT TACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC ATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAG CTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATC CGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCAC TGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGT GCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGAC AGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAG TTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTT TTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGA TCCTTTGATCTTTTCTAC >M13R GGAAACAGCTATGACCATGA >T3_primer AATTAACCCTCACTAAAGGG >Tyro_frag_ex5 GATCTGCTGGGCTTGCTGAAGGTAGGGGGTCAAGAAC >5'_TIR CAGAGGTGTAAAGTACTTG >517_bp_of_Tol2_5'_end CAGAGGTGTAAAGTACTTGAGTAATTTTACTTGATTACTGTACTTAAGTA TTATTTTTGGGGATTTTTACTTTACTTGAGTACAATTAAAAATCAATACT TTTACTTTTACTTAATTACATTTTTTTAGAAAAAAAAGTACTTTTTACTC CTTACAATTTTATTTACAGTCAAAAAGTACTTATTTTTTGGAGATCACTT CATTCTATTTTCCCTTGCTATTACCAAACCAATTGAATTGCGCTGATGCC CAGTTTAATTTAAATGTTATTTATTCTGCCTATGAAAATCGTTTTCACAT TATATGAAATTGGTCAGACATGTTCATTGGTCCTTTGGAAGTGACGTCAT GTCACATCTATTACCACAATGCACAGCACCTTGACCTGGAAATTAGGGAA ATTATAACAGTCAATCAGTGGAAGAAAATGGAGGAAGTATGTGATTCATC AGCAGCTGCGAGCAGCACAGTCCAAAATCAGCCACAGGATCAAGAGCACC CGTGGCCGTATCTTCGC >Tol2_exon1,_bp1-335_(of_461) ATTTTTTGGAGATCACTTCATTCTATTTTCCCTTGCTATTACCAAACCAA TTGAATTGCGCTGATGCCCAGTTTAATTTAAATGTTATTTATTCTGCCTA TGAAAATCGTTTTCACATTATATGAAATTGGTCAGACATGTTCATTGGTC CTTTGGAAGTGACGTCATGTCACATCTATTACCACAATGCACAGCACCTT GACCTGGAAATTAGGGAAATTATAACAGTCAATCAGTGGAAGAAAATGGA GGAAGTATGTGATTCATCAGCAGCTGCGAGCAGCACAGTCCAAAATCAGC CACAGGATCAAGAGCACCCGTGGCCGTATCTTCGC >SV40_late_polyA GATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAG AATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTT TATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGC ATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAG CAAGTAAAACCTCTACAAATGTGGTATGGCTGATTAT >M13_-20 GTAAAACGACGGCCAGTG >attR3 CCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTT TATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCT CGTTCAACTTTATTATACATAGTTG >attB3/L3/R3_shared CAACTTTATTATACA >cmR TTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCA TTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCC AGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAA AACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGG TGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAAC CCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGA ATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCG ATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACA CTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGG ATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACT TGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGA ACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATG TTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTT TTTTCTCCAT >ccdB_from_pDEST_R4-R3_(1_mutation) ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTT TGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGA TCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCCCCCGTGAACTT TACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGA TATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCA GCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGA ATATAA >attR4 CAACTTTGTATAGAAAAGTTGAACGAGAAACGTAAAATGATATAAATATC AATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAA AACACAACATATCCAGTCACTATGG >attB4/L4/R4_shared CAACTTTGTATAGAAAAGTTG >M13R GGAAACAGCTATGACCATGA >Tol2_exon4_136-482 ATCTGGCCTGTGTTTCAGACACCAGGGAGTCTCTGCTCACGTTTCCTGCT ATTTGCAGCCTCTCTATCAAGACTAATACACCTCTTCCCGCATCGGCTGC CTGTGAGAGGCTTTTCAGCACTGCAGGATTGCTTTTCAGCCCCAAAAGAG CTAGGCTTGACACTAACAATTTTGAGAATCAGCTTCTACTGAAGTTAAAT CTGAGGTTTTACAACTTTGAGTAGCGTGTACTGGCATTAGATTGTCTGTC TTATAGTTTGATAATTAAATACAAACAGTTCTAAAGCAGGATAAAACCTT GTATGCATTTCATTTAATGTTTTTTGAGATTAAAAGCTTAAACAAGA >536_bp_of_Tol2_3'_end ATCTGGCCTGTGTTTCAGACACCAGGGAGTCTCTGCTCACGTTTCCTGCT ATTTGCAGCCTCTCTATCAAGACTAATACACCTCTTCCCGCATCGGCTGC CTGTGAGAGGCTTTTCAGCACTGCAGGATTGCTTTTCAGCCCCAAAAGAG CTAGGCTTGACACTAACAATTTTGAGAATCAGCTTCTACTGAAGTTAAAT CTGAGGTTTTACAACTTTGAGTAGCGTGTACTGGCATTAGATTGTCTGTC TTATAGTTTGATAATTAAATACAAACAGTTCTAAAGCAGGATAAAACCTT GTATGCATTTCATTTAATGTTTTTTGAGATTAAAAGCTTAAACAAGAATC TCTAGTTTTCTTTCTTGCTTTTACTTTTACTTCCTTAATACTCAAGTACA ATTTTAATGGAGTACTTTTTTACTTTTACTCAAGTAAGATTCTAGCCAGA TACTTTTACTTTTAATTGAGTAAAATTTTCCCTAAGTACTTGTACTTTCA CTTGAGTAAAATTTTTGAGTACTTTTTACACCTCTG >Tol2_primer AGGCTGCAAATAGCAGGAAA >3'_TIR TACTTTTTACACCTCTG >tyrosinase_genomic_from_AB010101 GGTACCAAATAGTAGGAATTACCCACCTGTACAAGTGCTGAAAACTTGGA TGAATAAGCCCGTTTGCCATTTTCTACTGCTATTTTAAATCTTTTCTGTA TTTGTCTGCATTTGTCTTTACCCTTGAATAAATGTTTTAGTTGTTTTTTT TCAATTATGACTGTGTTTAACAGACATTATTACAATAATATGTAATAGTA GTACACACTATTATTATGTAATAGTACTTGTTGACTGTATTTGAGAACTG GACCGAGTGAGTGTTACGTCACCCATTCAAAATGACTTACTTCTGGCTCC AACAAAATGAAGTTAATTCAGTTGCCATTTTTCACTGTATGGACATCGCC GTGTTGGAGCTAGACGTCGCCAATAAGCAAGAAAGAGCCGAGATGCGTCG AGTCTGAGTCACCGTTCCTATGGCAACCCCTCTAACCAATCAGAAGTAAG CTTGTTGGAAGTCCACAGCCTACCACTTGAAAGCGGGCTGCACAAAATCT GTCAAACGTTTTGAACGTTGGATGTGAGAGCACATACTTTTATTAAGGCA TCTGGTTGGTCAGTTTATAATATCAACAACTTGGGCTACAGAAAAGAAAA GTTATTACAGAAATTATGATTAACAAGTACATGTTAAATAAAGATTTTAA TATGAATGCCACCACTGGAGCATTCATGCCATTTGGAGCTTCTTCCTGTT TGGATCACTAGAAGGAGGAGGTCACTCATTACAGTTCTCATATACAGTCG TTGGTTGGTTGGTTGGTTGGTTGGTTGGTAGATTGATTGATTGATTGATT GATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGA TTGATTGATTGATTGATTGATTGATTGATTGATTGGTAGTCAAAATAAGA AATAATTTCCACAGATTCATTACAGAAATGATTAAATGCATACATAAAAA ACTGGGGGGGGGGGGATACAACAACACACTTAAGTCACATTTGCCTACGT AAAGAAAAGTAAAGAAAATCAATAGCTATATTTTACATCTCCTTTTTTTG CTGTCTTTTAATTAGCCTTGTTTTGCTGTTATCTTGATTGAAACTGTAAC TTCTTCACCTGCTTCTTTTCTTTGTAGGTTTTGCCAGCCCAGAAACAGGC ATGGCTGTGCAAGGCCAGAGCACCATGCACAATGCGCTGCATGTCTTCAT GAACGGCTCAATGTCCTCAGTCCAGGGCTCAGCCAACGACCCCATCTTCC TCCTTCACCATGCTTTCATTGACAGGTAACAAACACGTCATGACATTAGA CTGCACAGTTTTTGACAAAGTTCATACAATCTGTTGTTTATAGCTGCTAC AATTAGTGAAGTTTGTGAATGTACTTGGATGAGCAGCGAAAGATCAATTG AGATCAATTGTTAGAGTTTGGTTGCCCTGCAGAGCAAAGAACAAAAAATA ATCTGGTGGCTTTACTGCGTGAGGTTATTATTGGTGGAATAGAAACACAA AACATAATTGCATTTATTTGTTTAATTTTTTATCTTATCTTAACTTTCAT CTTGCATATTTGTTTCTTACATCATTTCTAGCATCTTTGAGCGCTGGCTA AGAACTCATCAGCCTCCCCGGTCCATCTACCCACGTACCAATGCACCAAT TGGCCACAATGACGGCTACTACATGGTGCCATTCCTTCCTCTTTATAGGA ATGGAGACTACCTCCTGTCCAACAAGGCTCTTGGATACGAGTACGCCTAC CTGTTGGACCCAGGTCATTGCACAACACCAGAAATGCCCTCTGATCTGCA AAAGACGTGAATATCTGTTCAGACACCCATATCCACTCTGTTCCACACAG GTCAGAGGTTTGTCCAGGAGTTCTTGAC >Tyro_frag_ex4-in4-ex5 CAGGTCATTGCACAACACCAGAAATGCCCTCTGATCTGCAAAAGACGTGA ATATCTGTTCAGACACCCATATCCACTCTGTTCCACACAGG >pBSII_SK+_5’_of_KpnI CCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTT TGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCC TTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTT GGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGA AAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATC AAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAG GGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGA AAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGT AGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGC TACAGGGCGCGTCCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGG CGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATG TGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGAC GTTGTAAAACGACGGCCAGTGAGCGCGCGTAATACGACTCACTATAGGGC GAATTGGGTACC >T7_primer GTAATACGACTCACTATAGGGCG >M13_-20 GTAAAACGACGGCCAGTG >f1_(+)_ori GCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACT TGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCG CCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTA GGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTA GGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCC CTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACT GGAACAA