From Tol2Kit
| Line 97: | Line 97: | ||
GTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGC | GTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGC | ||
AAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTC | AAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTC | ||
| − | + | AACTTTATTATACATAGTTGATAATTCACTGGCCGTCGTTTTACGGTACC | |
| − | + | ATCGATGATGATCCAGACATGATAAGATACATTGATGAGTTTGGACAAAC | |
| − | + | CACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATG | |
| − | + | CTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAAC | |
| − | + | AACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGT | |
| − | + | TTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTATGGCTGATTATGATC | |
| − | + | CTCTAGATCAGATCTGCGAAGATACGGCCACGGGTGCTCTTGATCCTGTG | |
| − | + | GCTGATTTTGGACTGTGCTGCTCGCAGCTGCTGATGAATCACATACTTCC | |
| − | + | TCCATTTTCTTCCACTGATTGACTGTTATAATTTCCCTAATTTCCAGGTC | |
| − | + | AAGGTGCTGTGCATTGTGGTAATAGATGTGACATGACGTCACTTCCAAAG | |
| − | + | GACCAATGAACATGTCTGACCAATTTCATATAATGTGAAAACGATTTTCA | |
| − | + | TAGGCAGAATAAATAACATTTAAATTAAACTGGGCATCAGCGCAATTCAA | |
| − | + | TTGGTTTGGTAATAGCAAGGGAAAATAGAATGAAGTGATCTCCAAAAAAT | |
| − | + | AAGTACTTTTTGACTGTAAATAAAATTGTAAGGAGTAAAAAGTACTTTTT | |
| − | + | TTTCTAAAAAAATGTAATTAAGTAAAAGTAAAAGTATTGATTTTTAATTG | |
| − | + | TACTCAAGTAAAGTAAAAATCCCCAAAAATAATACTTAAGTACAGTAATC | |
| − | + | AAGTAAAATTACTCAAGTACTTTACACCTCTGGTTCTTGACCCCCTACCT | |
| − | + | TCAGCAAGCCCAGCAGATCCACTAGTTCTAGAGCGGCCGCCACCGCGGTG | |
| − | + | GAGCTCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGCGCTTGGCGT | |
| − | + | AATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATT | |
| − | + | CCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTA | |
| − | + | ATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCC | |
| − | + | AGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCG | |
| − | + | GGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGA | |
| − | + | CTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAA | |
| − | + | AGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAAC | |
| − | + | ATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTT | |
| − | + | GCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATC | |
| − | + | GACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAG | |
| − | + | GCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCC | |
| − | + | GCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTT | |
| − | + | CTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCC | |
| − | + | AAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTT | |
| − | + | ATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGC | |
| − | + | CACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGC | |
| − | + | GGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAG | |
| − | + | GACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAA | |
| − | + | GAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGT | |
| − | + | TTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGA | |
| − | + | AGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACT | |
| − | + | CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAG | |
| − | + | ATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGA | |
| − | + | GTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCT | |
| − | + | CAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTG | |
| − | + | TAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAAT | |
| − | + | GATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACC | |
| − | + | AGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCC | |
| − | + | TCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCC | |
| − | + | AGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGT | |
| − | + | CACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCA | |
| − | + | AGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTT | |
| − | + | CGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCA | |
| − | + | TGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGA | |
| − | + | TGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTG | |
| − | + | TATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCG | |
| − | + | CGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCG | |
| − | + | GGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTA | |
| − | + | ACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCG | |
| − | + | TTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATA | |
| − | + | AGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTA | |
| − | + | TTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAAT | |
| − | + | GTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAA | |
| + | GTG | ||
| + | >pBSII_SK+_5’_of_KpnI | ||
| + | CCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTT | ||
| + | TGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCC | ||
| + | TTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTT | ||
| + | GGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGA | ||
| + | AAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATC | ||
| + | AAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAG | ||
| + | GGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGA | ||
| + | AAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGT | ||
| + | AGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGC | ||
| + | TACAGGGCGCGTCCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGG | ||
| + | CGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATG | ||
| + | TGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGAC | ||
| + | GTTGTAAAACGACGGCCAGTGAGCGCGCGTAATACGACTCACTATAGGGC | ||
| + | GAATTGGGTACC | ||
| + | >f1_(+)_ori | ||
| + | GCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACT | ||
| + | TGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCG | ||
| + | CCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTA | ||
| + | GGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTA | ||
| + | GGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCC | ||
| + | CTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACT | ||
| + | GGAACAA | ||
| + | >M13_-20 | ||
| + | GTAAAACGACGGCCAGTG | ||
| + | >T7_primer | ||
| + | GTAATACGACTCACTATAGGGCG | ||
| + | >tyrosinase_genomic_from_AB010101 | ||
| + | GGTACCAAATAGTAGGAATTACCCACCTGTACAAGTGCTGAAAACTTGGA | ||
| + | TGAATAAGCCCGTTTGCCATTTTCTACTGCTATTTTAAATCTTTTCTGTA | ||
| + | TTTGTCTGCATTTGTCTTTACCCTTGAATAAATGTTTTAGTTGTTTTTTT | ||
| + | TCAATTATGACTGTGTTTAACAGACATTATTACAATAATATGTAATAGTA | ||
| + | GTACACACTATTATTATGTAATAGTACTTGTTGACTGTATTTGAGAACTG | ||
| + | GACCGAGTGAGTGTTACGTCACCCATTCAAAATGACTTACTTCTGGCTCC | ||
| + | AACAAAATGAAGTTAATTCAGTTGCCATTTTTCACTGTATGGACATCGCC | ||
| + | GTGTTGGAGCTAGACGTCGCCAATAAGCAAGAAAGAGCCGAGATGCGTCG | ||
| + | AGTCTGAGTCACCGTTCCTATGGCAACCCCTCTAACCAATCAGAAGTAAG | ||
| + | CTTGTTGGAAGTCCACAGCCTACCACTTGAAAGCGGGCTGCACAAAATCT | ||
| + | GTCAAACGTTTTGAACGTTGGATGTGAGAGCACATACTTTTATTAAGGCA | ||
| + | TCTGGTTGGTCAGTTTATAATATCAACAACTTGGGCTACAGAAAAGAAAA | ||
| + | GTTATTACAGAAATTATGATTAACAAGTACATGTTAAATAAAGATTTTAA | ||
| + | TATGAATGCCACCACTGGAGCATTCATGCCATTTGGAGCTTCTTCCTGTT | ||
| + | TGGATCACTAGAAGGAGGAGGTCACTCATTACAGTTCTCATATACAGTCG | ||
| + | TTGGTTGGTTGGTTGGTTGGTTGGTTGGTAGATTGATTGATTGATTGATT | ||
| + | GATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGA | ||
| + | TTGATTGATTGATTGATTGATTGATTGATTGATTGGTAGTCAAAATAAGA | ||
| + | AATAATTTCCACAGATTCATTACAGAAATGATTAAATGCATACATAAAAA | ||
| + | ACTGGGGGGGGGGGGATACAACAACACACTTAAGTCACATTTGCCTACGT | ||
| + | AAAGAAAAGTAAAGAAAATCAATAGCTATATTTTACATCTCCTTTTTTTG | ||
| + | CTGTCTTTTAATTAGCCTTGTTTTGCTGTTATCTTGATTGAAACTGTAAC | ||
| + | TTCTTCACCTGCTTCTTTTCTTTGTAGGTTTTGCCAGCCCAGAAACAGGC | ||
| + | ATGGCTGTGCAAGGCCAGAGCACCATGCACAATGCGCTGCATGTCTTCAT | ||
| + | GAACGGCTCAATGTCCTCAGTCCAGGGCTCAGCCAACGACCCCATCTTCC | ||
| + | TCCTTCACCATGCTTTCATTGACAGGTAACAAACACGTCATGACATTAGA | ||
| + | CTGCACAGTTTTTGACAAAGTTCATACAATCTGTTGTTTATAGCTGCTAC | ||
| + | AATTAGTGAAGTTTGTGAATGTACTTGGATGAGCAGCGAAAGATCAATTG | ||
| + | AGATCAATTGTTAGAGTTTGGTTGCCCTGCAGAGCAAAGAACAAAAAATA | ||
| + | ATCTGGTGGCTTTACTGCGTGAGGTTATTATTGGTGGAATAGAAACACAA | ||
| + | AACATAATTGCATTTATTTGTTTAATTTTTTATCTTATCTTAACTTTCAT | ||
| + | CTTGCATATTTGTTTCTTACATCATTTCTAGCATCTTTGAGCGCTGGCTA | ||
| + | AGAACTCATCAGCCTCCCCGGTCCATCTACCCACGTACCAATGCACCAAT | ||
| + | TGGCCACAATGACGGCTACTACATGGTGCCATTCCTTCCTCTTTATAGGA | ||
| + | ATGGAGACTACCTCCTGTCCAACAAGGCTCTTGGATACGAGTACGCCTAC | ||
| + | CTGTTGGACCCAGGTCATTGCACAACACCAGAAATGCCCTCTGATCTGCA | ||
| + | AAAGACGTGAATATCTGTTCAGACACCCATATCCACTCTGTTCCACACAG | ||
| + | GTCAGAGGTTTGTCCAGGAGTTCTTGAC | ||
| + | >Tyro_frag_ex4-in4-ex5 | ||
| + | CAGGTCATTGCACAACACCAGAAATGCCCTCTGATCTGCAAAAGACGTGA | ||
| + | ATATCTGTTCAGACACCCATATCCACTCTGTTCCACACAGG | ||
| + | >3'_TIR | ||
| + | TACTTTTTACACCTCTG | ||
| + | >536_bp_of_Tol2_3'_end | ||
| + | ATCTGGCCTGTGTTTCAGACACCAGGGAGTCTCTGCTCACGTTTCCTGCT | ||
| + | ATTTGCAGCCTCTCTATCAAGACTAATACACCTCTTCCCGCATCGGCTGC | ||
| + | CTGTGAGAGGCTTTTCAGCACTGCAGGATTGCTTTTCAGCCCCAAAAGAG | ||
| + | CTAGGCTTGACACTAACAATTTTGAGAATCAGCTTCTACTGAAGTTAAAT | ||
| + | CTGAGGTTTTACAACTTTGAGTAGCGTGTACTGGCATTAGATTGTCTGTC | ||
| + | TTATAGTTTGATAATTAAATACAAACAGTTCTAAAGCAGGATAAAACCTT | ||
| + | GTATGCATTTCATTTAATGTTTTTTGAGATTAAAAGCTTAAACAAGAATC | ||
| + | TCTAGTTTTCTTTCTTGCTTTTACTTTTACTTCCTTAATACTCAAGTACA | ||
| + | ATTTTAATGGAGTACTTTTTTACTTTTACTCAAGTAAGATTCTAGCCAGA | ||
| + | TACTTTTACTTTTAATTGAGTAAAATTTTCCCTAAGTACTTGTACTTTCA | ||
| + | CTTGAGTAAAATTTTTGAGTACTTTTTACACCTCTG | ||
| + | >Tol2_exon4_136-482 | ||
| + | ATCTGGCCTGTGTTTCAGACACCAGGGAGTCTCTGCTCACGTTTCCTGCT | ||
| + | ATTTGCAGCCTCTCTATCAAGACTAATACACCTCTTCCCGCATCGGCTGC | ||
| + | CTGTGAGAGGCTTTTCAGCACTGCAGGATTGCTTTTCAGCCCCAAAAGAG | ||
| + | CTAGGCTTGACACTAACAATTTTGAGAATCAGCTTCTACTGAAGTTAAAT | ||
| + | CTGAGGTTTTACAACTTTGAGTAGCGTGTACTGGCATTAGATTGTCTGTC | ||
| + | TTATAGTTTGATAATTAAATACAAACAGTTCTAAAGCAGGATAAAACCTT | ||
| + | GTATGCATTTCATTTAATGTTTTTTGAGATTAAAAGCTTAAACAAGA | ||
| + | >Tol2_primer | ||
| + | AGGCTGCAAATAGCAGGAAA | ||
| + | >M13R | ||
| + | GGAAACAGCTATGACCATGA | ||
| + | >attB4/L4/R4_shared | ||
| + | CAACTTTGTATAGAAAAGTTG | ||
| + | >attR4 | ||
| + | CAACTTTGTATAGAAAAGTTGAACGAGAAACGTAAAATGATATAAATATC | ||
| + | AATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAA | ||
| + | AACACAACATATCCAGTCACTATGG | ||
| + | >ccdB_from_pDEST_R4-R3_(1_mutation) | ||
| + | ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTT | ||
| + | TGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGA | ||
| + | TCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCCCCCGTGAACTT | ||
| + | TACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGA | ||
| + | TATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCA | ||
| + | GCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGA | ||
| + | ATATAA | ||
| + | >cmR | ||
| + | TTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCA | ||
| + | TTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCC | ||
| + | AGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAA | ||
| + | AACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGG | ||
| + | TGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAAC | ||
| + | CCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGA | ||
| + | ATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCG | ||
| + | ATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACA | ||
| + | CTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGG | ||
| + | ATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACT | ||
| + | TGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGA | ||
| + | ACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATG | ||
| + | TTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTT | ||
| + | TTTTCTCCAT | ||
| + | >attR3 | ||
| + | CCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTT | ||
| + | TATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCT | ||
| + | CGTTCAACTTTATTATACATAGTTG | ||
| + | >attB3/L3/R3_shared | ||
| + | CAACTTTATTATACA | ||
| + | >M13_-20 | ||
| + | GTAAAACGACGGCCAGTG | ||
| + | >SV40_late_polyA | ||
| + | GATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAG | ||
| + | AATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTT | ||
| + | TATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGC | ||
| + | ATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAG | ||
| + | CAAGTAAAACCTCTACAAATGTGGTATGGCTGATTAT | ||
| + | >Tol2_exon1,_bp1-335_(of_461) | ||
| + | ATTTTTTGGAGATCACTTCATTCTATTTTCCCTTGCTATTACCAAACCAA | ||
| + | TTGAATTGCGCTGATGCCCAGTTTAATTTAAATGTTATTTATTCTGCCTA | ||
| + | TGAAAATCGTTTTCACATTATATGAAATTGGTCAGACATGTTCATTGGTC | ||
| + | CTTTGGAAGTGACGTCATGTCACATCTATTACCACAATGCACAGCACCTT | ||
| + | GACCTGGAAATTAGGGAAATTATAACAGTCAATCAGTGGAAGAAAATGGA | ||
| + | GGAAGTATGTGATTCATCAGCAGCTGCGAGCAGCACAGTCCAAAATCAGC | ||
| + | CACAGGATCAAGAGCACCCGTGGCCGTATCTTCGC | ||
| + | >517_bp_of_Tol2_5'_end | ||
| + | CAGAGGTGTAAAGTACTTGAGTAATTTTACTTGATTACTGTACTTAAGTA | ||
| + | TTATTTTTGGGGATTTTTACTTTACTTGAGTACAATTAAAAATCAATACT | ||
| + | TTTACTTTTACTTAATTACATTTTTTTAGAAAAAAAAGTACTTTTTACTC | ||
| + | CTTACAATTTTATTTACAGTCAAAAAGTACTTATTTTTTGGAGATCACTT | ||
| + | CATTCTATTTTCCCTTGCTATTACCAAACCAATTGAATTGCGCTGATGCC | ||
| + | CAGTTTAATTTAAATGTTATTTATTCTGCCTATGAAAATCGTTTTCACAT | ||
| + | TATATGAAATTGGTCAGACATGTTCATTGGTCCTTTGGAAGTGACGTCAT | ||
| + | GTCACATCTATTACCACAATGCACAGCACCTTGACCTGGAAATTAGGGAA | ||
| + | ATTATAACAGTCAATCAGTGGAAGAAAATGGAGGAAGTATGTGATTCATC | ||
| + | AGCAGCTGCGAGCAGCACAGTCCAAAATCAGCCACAGGATCAAGAGCACC | ||
| + | CGTGGCCGTATCTTCGC | ||
| + | >5'_TIR | ||
| + | CAGAGGTGTAAAGTACTTG | ||
| + | >Tyro_frag_ex5 | ||
| + | GATCTGCTGGGCTTGCTGAAGGTAGGGGGTCAAGAAC | ||
>pBSII_SK+_3’_of_SpeI | >pBSII_SK+_3’_of_SpeI | ||
ACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTCCAGCTTTTGTTCCC | ACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTCCAGCTTTTGTTCCC | ||
| Line 205: | Line 368: | ||
ATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAA | ATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAA | ||
ATAGGGGTTCCGCGCACATTTCCCCGAAAAGTG | ATAGGGGTTCCGCGCACATTTCCCCGAAAAGTG | ||
| + | >T3_primer | ||
| + | AATTAACCCTCACTAAAGGG | ||
| + | >M13R | ||
| + | GGAAACAGCTATGACCATGA | ||
| + | >pUC_ori | ||
| + | TGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCT | ||
| + | GGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGAC | ||
| + | GCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCG | ||
| + | TTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCT | ||
| + | TACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC | ||
| + | ATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAG | ||
| + | CTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATC | ||
| + | CGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCAC | ||
| + | TGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGT | ||
| + | GCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGAC | ||
| + | AGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAG | ||
| + | TTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTT | ||
| + | TTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGA | ||
| + | TCCTTTGATCTTTTCTAC | ||
>amp | >amp | ||
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATT | ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATT | ||
| Line 224: | Line 406: | ||
TATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTA | TATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTA | ||
AGCATTGG | AGCATTGG | ||
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</pre> | </pre> | ||
Latest revision as of 17:42, 7 June 2007
>pDest-Tol2-pA CCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTT TGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCC TTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTT GGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGA AAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATC AAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAG GGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGA AAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGT AGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGC TACAGGGCGCGTCCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGG CGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATG TGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGAC GTTGTAAAACGACGGCCAGTGAGCGCGCGTAATACGACTCACTATAGGGC GAATTGGGTACCAAATAGTAGGAATTACCCACCTGTACAAGTGCTGAAAA CTTGGATGAATAAGCCCGTTTGCCATTTTCTACTGCTATTTTAAATCTTT TCTGTATTTGTCTGCATTTGTCTTTACCCTTGAATAAATGTTTTAGTTGT TTTTTTTCAATTATGACTGTGTTTAACAGACATTATTACAATAATATGTA ATAGTAGTACACACTATTATTATGTAATAGTACTTGTTGACTGTATTTGA GAACTGGACCGAGTGAGTGTTACGTCACCCATTCAAAATGACTTACTTCT GGCTCCAACAAAATGAAGTTAATTCAGTTGCCATTTTTCACTGTATGGAC ATCGCCGTGTTGGAGCTAGACGTCGCCAATAAGCAAGAAAGAGCCGAGAT GCGTCGAGTCTGAGTCACCGTTCCTATGGCAACCCCTCTAACCAATCAGA AGTAAGCTTGTTGGAAGTCCACAGCCTACCACTTGAAAGCGGGCTGCACA AAATCTGTCAAACGTTTTGAACGTTGGATGTGAGAGCACATACTTTTATT AAGGCATCTGGTTGGTCAGTTTATAATATCAACAACTTGGGCTACAGAAA AGAAAAGTTATTACAGAAATTATGATTAACAAGTACATGTTAAATAAAGA TTTTAATATGAATGCCACCACTGGAGCATTCATGCCATTTGGAGCTTCTT CCTGTTTGGATCACTAGAAGGAGGAGGTCACTCATTACAGTTCTCATATA CAGTCGTTGGTTGGTTGGTTGGTTGGTTGGTTGGTAGATTGATTGATTGA TTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATT GATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGGTAGTCAAA ATAAGAAATAATTTCCACAGATTCATTACAGAAATGATTAAATGCATACA TAAAAAACTGGGGGGGGGGGGATACAACAACACACTTAAGTCACATTTGC CTACGTAAAGAAAAGTAAAGAAAATCAATAGCTATATTTTACATCTCCTT TTTTTGCTGTCTTTTAATTAGCCTTGTTTTGCTGTTATCTTGATTGAAAC TGTAACTTCTTCACCTGCTTCTTTTCTTTGTAGGTTTTGCCAGCCCAGAA ACAGGCATGGCTGTGCAAGGCCAGAGCACCATGCACAATGCGCTGCATGT CTTCATGAACGGCTCAATGTCCTCAGTCCAGGGCTCAGCCAACGACCCCA TCTTCCTCCTTCACCATGCTTTCATTGACAGGTAACAAACACGTCATGAC ATTAGACTGCACAGTTTTTGACAAAGTTCATACAATCTGTTGTTTATAGC TGCTACAATTAGTGAAGTTTGTGAATGTACTTGGATGAGCAGCGAAAGAT CAATTGAGATCAATTGTTAGAGTTTGGTTGCCCTGCAGAGCAAAGAACAA AAAATAATCTGGTGGCTTTACTGCGTGAGGTTATTATTGGTGGAATAGAA ACACAAAACATAATTGCATTTATTTGTTTAATTTTTTATCTTATCTTAAC TTTCATCTTGCATATTTGTTTCTTACATCATTTCTAGCATCTTTGAGCGC TGGCTAAGAACTCATCAGCCTCCCCGGTCCATCTACCCACGTACCAATGC ACCAATTGGCCACAATGACGGCTACTACATGGTGCCATTCCTTCCTCTTT ATAGGAATGGAGACTACCTCCTGTCCAACAAGGCTCTTGGATACGAGTAC GCCTACCTGTTGGACCCAGGTCATTGCACAACACCAGAAATGCCCTCTGA TCTGCAAAAGACGTGAATATCTGTTCAGACACCCATATCCACTCTGTTCC ACACAGGTCAGAGGTTTGTCCAGGAGTTCTTGACAGAGGTGTAAAAAGTA CTCAAAAATTTTACTCAAGTGAAAGTACAAGTACTTAGGGAAAATTTTAC TCAATTAAAAGTAAAAGTATCTGGCTAGAATCTTACTTGAGTAAAAGTAA AAAAGTACTCCATTAAAATTGTACTTGAGTATTAAGGAAGTAAAAGTAAA AGCAAGAAAGAAAACTAGAGATTCTTGTTTAAGCTTTTAATCTCAAAAAA CATTAAATGAAATGCATACAAGGTTTTATCCTGCTTTAGAACTGTTTGTA TTTAATTATCAAACTATAAGACAGACAATCTAATGCCAGTACACGCTACT CAAAGTTGTAAAACCTCAGATTTAACTTCAGTAGAAGCTGATTCTCAAAA TTGTTAGTGTCAAGCCTAGCTCTTTTGGGGCTGAAAAGCAATCCTGCAGT GCTGAAAAGCCTCTCACAGGCAGCCGATGCGGGAAGAGGTGTATTAGTCT TGATAGAGAGGCTGCAAATAGCAGGAAACGTGAGCAGAGACTCCCTGGTG TCTGAAACACAGGCCAGATGGGCCCTCGAGCAGGAAACAGCTATGACCAT GATTACGCCAAGCTATCAACTTTGTATAGAAAAGTTGAACGAGAAACGTA AAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGA CTACATAATACTGTAAAACACAACATATCCAGTCACTATGGTCGACCTGC AGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCA GGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACT TCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCAT GCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGGGA CTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGC CCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACG GCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGCCCCTG TTCTCGTCGGCAAAGGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGC CATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGG CTTCATTCTGCATGGTTGTGCTTACCGAACCGGAGATATTGACATCATAT ATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGC TGCTTCATAGCATACCTCTTTTTGACATACTTCGGGTATACATATCAGTA TATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCT GGCTTTTAGTAAGCCGGATCCTCTAGATTACGCCCCGCCCTGCCACTCAT CGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCA CAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCC TTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCA TATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCT GAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTT TTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGA AATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCA TGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACC GTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAA GAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTC TTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTG AGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATA TATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTA GCTCCTGAAAATCTCGACGGATCCTAACTCAAAATCCACACATTATACGA GCCGGAAGCATAAAGTGTAAAGCCTGGGGGTGCCTAATGCGGCCGCCATA GTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGC AAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTC AACTTTATTATACATAGTTGATAATTCACTGGCCGTCGTTTTACGGTACC ATCGATGATGATCCAGACATGATAAGATACATTGATGAGTTTGGACAAAC CACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATG CTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAAC AACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGT TTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTATGGCTGATTATGATC CTCTAGATCAGATCTGCGAAGATACGGCCACGGGTGCTCTTGATCCTGTG GCTGATTTTGGACTGTGCTGCTCGCAGCTGCTGATGAATCACATACTTCC TCCATTTTCTTCCACTGATTGACTGTTATAATTTCCCTAATTTCCAGGTC AAGGTGCTGTGCATTGTGGTAATAGATGTGACATGACGTCACTTCCAAAG GACCAATGAACATGTCTGACCAATTTCATATAATGTGAAAACGATTTTCA TAGGCAGAATAAATAACATTTAAATTAAACTGGGCATCAGCGCAATTCAA TTGGTTTGGTAATAGCAAGGGAAAATAGAATGAAGTGATCTCCAAAAAAT AAGTACTTTTTGACTGTAAATAAAATTGTAAGGAGTAAAAAGTACTTTTT TTTCTAAAAAAATGTAATTAAGTAAAAGTAAAAGTATTGATTTTTAATTG TACTCAAGTAAAGTAAAAATCCCCAAAAATAATACTTAAGTACAGTAATC AAGTAAAATTACTCAAGTACTTTACACCTCTGGTTCTTGACCCCCTACCT TCAGCAAGCCCAGCAGATCCACTAGTTCTAGAGCGGCCGCCACCGCGGTG GAGCTCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGCGCTTGGCGT AATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATT CCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTA ATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCC AGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCG GGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGA CTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAA AGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAAC ATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTT GCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATC GACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAG GCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCC GCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTT CTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCC AAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTT ATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGC CACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGC GGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAG GACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAA GAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGT TTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGA AGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACT CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAG ATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGA GTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCT CAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTG TAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAAT GATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACC AGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCC TCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCC AGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGT CACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCA AGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTT CGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCA TGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGA TGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTG TATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCG CGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCG GGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTA ACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCG TTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATA AGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTA TTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAAT GTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAA GTG >pBSII_SK+_5’_of_KpnI CCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTT TGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCC TTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTT GGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGA AAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATC AAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAG GGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGA AAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGT AGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGC TACAGGGCGCGTCCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGG CGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATG TGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGAC GTTGTAAAACGACGGCCAGTGAGCGCGCGTAATACGACTCACTATAGGGC GAATTGGGTACC >f1_(+)_ori GCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACT TGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCG CCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTA GGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTA GGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCC CTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACT GGAACAA >M13_-20 GTAAAACGACGGCCAGTG >T7_primer GTAATACGACTCACTATAGGGCG >tyrosinase_genomic_from_AB010101 GGTACCAAATAGTAGGAATTACCCACCTGTACAAGTGCTGAAAACTTGGA TGAATAAGCCCGTTTGCCATTTTCTACTGCTATTTTAAATCTTTTCTGTA TTTGTCTGCATTTGTCTTTACCCTTGAATAAATGTTTTAGTTGTTTTTTT TCAATTATGACTGTGTTTAACAGACATTATTACAATAATATGTAATAGTA GTACACACTATTATTATGTAATAGTACTTGTTGACTGTATTTGAGAACTG GACCGAGTGAGTGTTACGTCACCCATTCAAAATGACTTACTTCTGGCTCC AACAAAATGAAGTTAATTCAGTTGCCATTTTTCACTGTATGGACATCGCC GTGTTGGAGCTAGACGTCGCCAATAAGCAAGAAAGAGCCGAGATGCGTCG AGTCTGAGTCACCGTTCCTATGGCAACCCCTCTAACCAATCAGAAGTAAG CTTGTTGGAAGTCCACAGCCTACCACTTGAAAGCGGGCTGCACAAAATCT GTCAAACGTTTTGAACGTTGGATGTGAGAGCACATACTTTTATTAAGGCA TCTGGTTGGTCAGTTTATAATATCAACAACTTGGGCTACAGAAAAGAAAA GTTATTACAGAAATTATGATTAACAAGTACATGTTAAATAAAGATTTTAA TATGAATGCCACCACTGGAGCATTCATGCCATTTGGAGCTTCTTCCTGTT TGGATCACTAGAAGGAGGAGGTCACTCATTACAGTTCTCATATACAGTCG TTGGTTGGTTGGTTGGTTGGTTGGTTGGTAGATTGATTGATTGATTGATT GATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGA TTGATTGATTGATTGATTGATTGATTGATTGATTGGTAGTCAAAATAAGA AATAATTTCCACAGATTCATTACAGAAATGATTAAATGCATACATAAAAA ACTGGGGGGGGGGGGATACAACAACACACTTAAGTCACATTTGCCTACGT AAAGAAAAGTAAAGAAAATCAATAGCTATATTTTACATCTCCTTTTTTTG CTGTCTTTTAATTAGCCTTGTTTTGCTGTTATCTTGATTGAAACTGTAAC TTCTTCACCTGCTTCTTTTCTTTGTAGGTTTTGCCAGCCCAGAAACAGGC ATGGCTGTGCAAGGCCAGAGCACCATGCACAATGCGCTGCATGTCTTCAT GAACGGCTCAATGTCCTCAGTCCAGGGCTCAGCCAACGACCCCATCTTCC TCCTTCACCATGCTTTCATTGACAGGTAACAAACACGTCATGACATTAGA CTGCACAGTTTTTGACAAAGTTCATACAATCTGTTGTTTATAGCTGCTAC AATTAGTGAAGTTTGTGAATGTACTTGGATGAGCAGCGAAAGATCAATTG AGATCAATTGTTAGAGTTTGGTTGCCCTGCAGAGCAAAGAACAAAAAATA ATCTGGTGGCTTTACTGCGTGAGGTTATTATTGGTGGAATAGAAACACAA AACATAATTGCATTTATTTGTTTAATTTTTTATCTTATCTTAACTTTCAT CTTGCATATTTGTTTCTTACATCATTTCTAGCATCTTTGAGCGCTGGCTA AGAACTCATCAGCCTCCCCGGTCCATCTACCCACGTACCAATGCACCAAT TGGCCACAATGACGGCTACTACATGGTGCCATTCCTTCCTCTTTATAGGA ATGGAGACTACCTCCTGTCCAACAAGGCTCTTGGATACGAGTACGCCTAC CTGTTGGACCCAGGTCATTGCACAACACCAGAAATGCCCTCTGATCTGCA AAAGACGTGAATATCTGTTCAGACACCCATATCCACTCTGTTCCACACAG GTCAGAGGTTTGTCCAGGAGTTCTTGAC >Tyro_frag_ex4-in4-ex5 CAGGTCATTGCACAACACCAGAAATGCCCTCTGATCTGCAAAAGACGTGA ATATCTGTTCAGACACCCATATCCACTCTGTTCCACACAGG >3'_TIR TACTTTTTACACCTCTG >536_bp_of_Tol2_3'_end ATCTGGCCTGTGTTTCAGACACCAGGGAGTCTCTGCTCACGTTTCCTGCT ATTTGCAGCCTCTCTATCAAGACTAATACACCTCTTCCCGCATCGGCTGC CTGTGAGAGGCTTTTCAGCACTGCAGGATTGCTTTTCAGCCCCAAAAGAG CTAGGCTTGACACTAACAATTTTGAGAATCAGCTTCTACTGAAGTTAAAT CTGAGGTTTTACAACTTTGAGTAGCGTGTACTGGCATTAGATTGTCTGTC TTATAGTTTGATAATTAAATACAAACAGTTCTAAAGCAGGATAAAACCTT GTATGCATTTCATTTAATGTTTTTTGAGATTAAAAGCTTAAACAAGAATC TCTAGTTTTCTTTCTTGCTTTTACTTTTACTTCCTTAATACTCAAGTACA ATTTTAATGGAGTACTTTTTTACTTTTACTCAAGTAAGATTCTAGCCAGA TACTTTTACTTTTAATTGAGTAAAATTTTCCCTAAGTACTTGTACTTTCA CTTGAGTAAAATTTTTGAGTACTTTTTACACCTCTG >Tol2_exon4_136-482 ATCTGGCCTGTGTTTCAGACACCAGGGAGTCTCTGCTCACGTTTCCTGCT ATTTGCAGCCTCTCTATCAAGACTAATACACCTCTTCCCGCATCGGCTGC CTGTGAGAGGCTTTTCAGCACTGCAGGATTGCTTTTCAGCCCCAAAAGAG CTAGGCTTGACACTAACAATTTTGAGAATCAGCTTCTACTGAAGTTAAAT CTGAGGTTTTACAACTTTGAGTAGCGTGTACTGGCATTAGATTGTCTGTC TTATAGTTTGATAATTAAATACAAACAGTTCTAAAGCAGGATAAAACCTT GTATGCATTTCATTTAATGTTTTTTGAGATTAAAAGCTTAAACAAGA >Tol2_primer AGGCTGCAAATAGCAGGAAA >M13R GGAAACAGCTATGACCATGA >attB4/L4/R4_shared CAACTTTGTATAGAAAAGTTG >attR4 CAACTTTGTATAGAAAAGTTGAACGAGAAACGTAAAATGATATAAATATC AATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAA AACACAACATATCCAGTCACTATGG >ccdB_from_pDEST_R4-R3_(1_mutation) ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTT TGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGA TCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCCCCCGTGAACTT TACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGA TATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCA GCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGA ATATAA >cmR TTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCA TTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCC AGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAA AACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGG TGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAAC CCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGA ATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCG ATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACA CTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGG ATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACT TGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGA ACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATG TTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTT TTTTCTCCAT >attR3 CCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTT TATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCT CGTTCAACTTTATTATACATAGTTG >attB3/L3/R3_shared CAACTTTATTATACA >M13_-20 GTAAAACGACGGCCAGTG >SV40_late_polyA GATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAG AATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTT TATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGC ATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAG CAAGTAAAACCTCTACAAATGTGGTATGGCTGATTAT >Tol2_exon1,_bp1-335_(of_461) ATTTTTTGGAGATCACTTCATTCTATTTTCCCTTGCTATTACCAAACCAA TTGAATTGCGCTGATGCCCAGTTTAATTTAAATGTTATTTATTCTGCCTA TGAAAATCGTTTTCACATTATATGAAATTGGTCAGACATGTTCATTGGTC CTTTGGAAGTGACGTCATGTCACATCTATTACCACAATGCACAGCACCTT GACCTGGAAATTAGGGAAATTATAACAGTCAATCAGTGGAAGAAAATGGA GGAAGTATGTGATTCATCAGCAGCTGCGAGCAGCACAGTCCAAAATCAGC CACAGGATCAAGAGCACCCGTGGCCGTATCTTCGC >517_bp_of_Tol2_5'_end CAGAGGTGTAAAGTACTTGAGTAATTTTACTTGATTACTGTACTTAAGTA TTATTTTTGGGGATTTTTACTTTACTTGAGTACAATTAAAAATCAATACT TTTACTTTTACTTAATTACATTTTTTTAGAAAAAAAAGTACTTTTTACTC CTTACAATTTTATTTACAGTCAAAAAGTACTTATTTTTTGGAGATCACTT CATTCTATTTTCCCTTGCTATTACCAAACCAATTGAATTGCGCTGATGCC CAGTTTAATTTAAATGTTATTTATTCTGCCTATGAAAATCGTTTTCACAT TATATGAAATTGGTCAGACATGTTCATTGGTCCTTTGGAAGTGACGTCAT GTCACATCTATTACCACAATGCACAGCACCTTGACCTGGAAATTAGGGAA ATTATAACAGTCAATCAGTGGAAGAAAATGGAGGAAGTATGTGATTCATC AGCAGCTGCGAGCAGCACAGTCCAAAATCAGCCACAGGATCAAGAGCACC CGTGGCCGTATCTTCGC >5'_TIR CAGAGGTGTAAAGTACTTG >Tyro_frag_ex5 GATCTGCTGGGCTTGCTGAAGGTAGGGGGTCAAGAAC >pBSII_SK+_3’_of_SpeI ACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTCCAGCTTTTGTTCCC TTTAGTGAGGGTTAATTGCGCGCTTGGCGTAATCATGGTCATAGCTGTTT CCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGG AAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACAT TAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGC CAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTAT TGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTC GGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCC ACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCA AAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGG CGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTC CCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCG CCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGG TATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGA ACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGT AACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAA GTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCG CTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCC GGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCA GATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTA CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTC ATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATG AAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTT ACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGT TCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGA GGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCT CACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAG CGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTG TTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACG TTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATG GCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCC CATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCA GAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCAT AATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGA GTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCT CTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTA AAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGAT CTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACT GATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACA GGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTG AATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTT ATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAA ATAGGGGTTCCGCGCACATTTCCCCGAAAAGTG >T3_primer AATTAACCCTCACTAAAGGG >M13R GGAAACAGCTATGACCATGA >pUC_ori TGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCT GGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGAC GCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCG TTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCT TACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC ATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAG CTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATC CGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCAC TGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGT GCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGAC AGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAG TTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTT TTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGA TCCTTTGATCTTTTCTAC >amp ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATT TTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATG CTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGAT GAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACG CCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGT AAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCA ACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCT GAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAA TGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACC ACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTG GAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAC TATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTA AGCATTGG