From Tol2Kit
(PME-mCherryCAAX moved to PME-mCherryCAAX H80D: adding page for pME-mCherryCAAX without mutation) |
|||
| Line 1: | Line 1: | ||
| − | + | === Construction details === | |
| + | pME-mCherryCAAX was made by PCR amplification of mCherry-CAAX, using primers to add att sites, followed by a BP reaction. mCherry-CAAX is mCherry with a C-terminal fusion of 21 amino acids of human Harvey Ras, encoding a prenylation signal. The original maxiprep that was distributed (clone 232) had an H80D point mutation. Nov 2007: The new version (clone 450) has had this mutation fixed and has no remaining mutations (thanks a lot to Seok-yong Choi). | ||
| + | |||
| + | === Crudely annotated sequence === | ||
| + | FASTA file with the full-length sequence as well as sequences of individual components:<br> | ||
| + | [[pME-mCherryCAAX sequence]] | ||
| + | |||
| + | === Crude map === | ||
| + | Screenshot from Sequencher showing locations of components:<br> | ||
| + | [[Image:pME-mCherryCAAX.png]] | ||
Revision as of 23:07, 2 November 2007
Construction details
pME-mCherryCAAX was made by PCR amplification of mCherry-CAAX, using primers to add att sites, followed by a BP reaction. mCherry-CAAX is mCherry with a C-terminal fusion of 21 amino acids of human Harvey Ras, encoding a prenylation signal. The original maxiprep that was distributed (clone 232) had an H80D point mutation. Nov 2007: The new version (clone 450) has had this mutation fixed and has no remaining mutations (thanks a lot to Seok-yong Choi).
Crudely annotated sequence
FASTA file with the full-length sequence as well as sequences of individual components:
pME-mCherryCAAX sequence
Crude map
Screenshot from Sequencher showing locations of components:
