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=== Construction details === | === Construction details === | ||
| − | p3E-IRES-nlsEGFPpA was made by fusion PCR of the EMCV IRES sequence, and nlsEGFP-SV40 late polyA, followed by a BP reaction. Sequencing confirms that all bases of the coding sequence are correct. There is a single base change in the IRES sequence, but we have confirmed functionally that this cassette produces fluorescent, nuclear-localized protein. | + | p3E-IRES-nlsEGFPpA was made by fusion PCR of the EMCV IRES sequence, and nlsEGFP-SV40 late polyA, followed by a BP reaction. Sequencing confirms that all bases of the coding sequence are correct. There is a single base change in the IRES sequence (G481A), but we have confirmed functionally that this cassette produces fluorescent, nuclear-localized protein. |
=== Crudely annotated sequence === | === Crudely annotated sequence === | ||
Revision as of 20:21, 10 December 2007
Construction details
p3E-IRES-nlsEGFPpA was made by fusion PCR of the EMCV IRES sequence, and nlsEGFP-SV40 late polyA, followed by a BP reaction. Sequencing confirms that all bases of the coding sequence are correct. There is a single base change in the IRES sequence (G481A), but we have confirmed functionally that this cassette produces fluorescent, nuclear-localized protein.
Crudely annotated sequence
FASTA file with the full-length sequence as well as sequences of individual components:
p3E-IRES-nlsEGFPpA sequence
Crude map
Screenshot from Sequencher showing locations of components:
