From Tol2Kit
(New page: === Construction details === pME-EGFP no stop was made by PCR amplification of the EGFP insert from a pCS2-based plasmid, using primers that add att sites, followed by a BP reaction. Sequ...) |
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This clone can be used to generate N-terminal EGFP fusions with a gene of interest placed in a 3' clone. | This clone can be used to generate N-terminal EGFP fusions with a gene of interest placed in a 3' clone. | ||
| − | === | + | === Sequence === |
| + | Annotated sequence, Genbank format:<br> | ||
| + | [[pME-EGFP no stop Genbank]] | ||
| + | |||
FASTA file with the full-length sequence as well as sequences of individual components:<br> | FASTA file with the full-length sequence as well as sequences of individual components:<br> | ||
[[pME-EGFP no stop sequence]] | [[pME-EGFP no stop sequence]] | ||
Latest revision as of 03:48, 19 December 2007
Construction details
pME-EGFP no stop was made by PCR amplification of the EGFP insert from a pCS2-based plasmid, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct.
This clone can be used to generate N-terminal EGFP fusions with a gene of interest placed in a 3' clone.
Sequence
Annotated sequence, Genbank format:
pME-EGFP no stop Genbank
FASTA file with the full-length sequence as well as sequences of individual components:
pME-EGFP no stop sequence
Crude map
Screenshot from Sequencher showing locations of components:
