From Tol2Kit
(New page: === Construction details === pME-mCherry no stop was made by PCR amplification of mCherry, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases ...) |
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pME-mCherry no stop was made by PCR amplification of mCherry, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct. | pME-mCherry no stop was made by PCR amplification of mCherry, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct. | ||
| − | === | + | This clone can be used to generate N-terminal mCherry fusions with a gene of interest placed in a 3' clone. |
| + | |||
| + | === Sequence === | ||
| + | Annotated sequence, Genbank format:<br> | ||
| + | [[pME-mCherry no stop Genbank]] | ||
| + | |||
FASTA file with the full-length sequence as well as sequences of individual components:<br> | FASTA file with the full-length sequence as well as sequences of individual components:<br> | ||
[[pME-mCherry no stop sequence]] | [[pME-mCherry no stop sequence]] | ||
Latest revision as of 03:49, 19 December 2007
Construction details
pME-mCherry no stop was made by PCR amplification of mCherry, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct.
This clone can be used to generate N-terminal mCherry fusions with a gene of interest placed in a 3' clone.
Sequence
Annotated sequence, Genbank format:
pME-mCherry no stop Genbank
FASTA file with the full-length sequence as well as sequences of individual components:
pME-mCherry no stop sequence
Crude map
Screenshot from Sequencher showing locations of components:
