From Tol2Kit
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<pre> | <pre> | ||
+ | LOCUS 396.pCS2-transposase 6034 bp ds-DNA circular 18-DEC-2007 | ||
+ | REFERENCE 1 | ||
+ | AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, | ||
+ | Parant JM, Yost HJ, Kanki JP, Chien CB. | ||
+ | TITLE The Tol2kit: a multisite gateway-based construction kit for Tol2 | ||
+ | transposon transgenesis constructs. | ||
+ | JOURNAL Developmental Dynamics 236(11):3088-99 (2007). | ||
+ | PUBMED 17937395 | ||
+ | COMMENT 396.pCS2-transposase, Tol2kit v1.0 | ||
+ | COMMENT ApEinfo:methylated:1 | ||
+ | FEATURES Location/Qualifiers | ||
+ | polyA_signal 3070..3267 | ||
+ | /label=SV40 late polyA (from pCS2+) | ||
+ | /ApEinfo_fwdcolor=#ffff7f | ||
+ | /ApEinfo_revcolor=#ffff7f | ||
+ | promoter 10..987 | ||
+ | /label=sCMV IE94 | ||
+ | /ApEinfo_fwdcolor=#ffffbf | ||
+ | /ApEinfo_revcolor=#ffffbf | ||
+ | rep_origin complement(3699..4372) | ||
+ | /label=pUC ori | ||
+ | /ApEinfo_fwdcolor=#999999 | ||
+ | /ApEinfo_revcolor=#999999 | ||
+ | rep_origin complement(3740..4422) | ||
+ | /label=colE1 ori | ||
+ | /ApEinfo_fwdcolor=#999999 | ||
+ | /ApEinfo_revcolor=#999999 | ||
+ | rep_origin complement(5640..5946) | ||
+ | /label=f1(+) ori | ||
+ | /ApEinfo_fwdcolor=#999999 | ||
+ | /ApEinfo_revcolor=#999999 | ||
+ | CDS complement(4520..5377) | ||
+ | /label=ampR | ||
+ | /ApEinfo_fwdcolor=#ff7f00 | ||
+ | /ApEinfo_revcolor=#ff7f00 | ||
+ | primer_bind complement(3351..3372) | ||
+ | /label=M13R | ||
+ | /ApEinfo_fwdcolor=#777777 | ||
+ | /ApEinfo_revcolor=#777777 | ||
+ | promoter complement(3316..3335) | ||
+ | /label=T3 | ||
+ | /ApEinfo_fwdcolor=#777777 | ||
+ | /ApEinfo_revcolor=#777777 | ||
+ | promoter 1022..1038 | ||
+ | /label=SP6 | ||
+ | /ApEinfo_fwdcolor=#ffff00 | ||
+ | /ApEinfo_revcolor=#ffff00 | ||
+ | misc_feature 1083..3032 | ||
+ | /label=Tol2 transposase cds | ||
+ | /ApEinfo_fwdcolor=cyan | ||
+ | /ApEinfo_revcolor=green | ||
+ | ORIGIN | ||
+ | 1 TCGACCATAG CCAATTCAAT ATGGCGTATA TGGACTCATG CCAATTCAAT ATGGTGGATC | ||
+ | 61 TGGACCTGTG CCAATTCAAT ATGGCGTATA TGGACTCGTG CCAATTCAAT ATGGTGGATC | ||
+ | 121 TGGACCCCAG CCAATTCAAT ATGGCGGACT TGGCACCATG CCAATTCAAT ATGGCGGACT | ||
+ | 181 TGGCACTGTG CCAACTGGGG AGGGGTCTAC TTGGCACGGT GCCAAGTTTG AGGAGGGGTC | ||
+ | 241 TTGGCCCTGT GCCAAGTCCG CCATATTGAA TTGGCATGGT GCCAATAATG GCGGCCATAT | ||
+ | 301 TGGCTATATG CCAGGATCAA TATATAGGCA ATATCCAATA TGGCCCTATG CCAATATGGC | ||
+ | 361 TATTGGCCAG GTTCAATACT ATGTATTGGC CCTATGCCAT ATAGTATTCC ATATATGGGT | ||
+ | 421 TTTCCTATTG ACGTAGATAG CCCCTCCCAA TGGGCGGTCC CATATACCAT ATATGGGGCT | ||
+ | 481 TCCTAATACC GCCCATAGCC ACTCCCCCAT TGACGTCAAT GGTCTCTATA TATGGTCTTT | ||
+ | 541 CCTATTGACG TCATATGGGC GGTCCTATTG ACGTATATGG CGCCTCCCCC ATTGACGTCA | ||
+ | 601 ATTACGGTAA ATGGCCCGCC TGGCTCAATG CCCATTGACG TCAATAGGAC CACCCACCAT | ||
+ | 661 TGACGTCAAT GGGATGGCTC ATTGCCCATT CATATCCGTT CTCACGCCCC CTATTGACGT | ||
+ | 721 CAATGACGGT AAATGGCCCA CTTGGCAGTA CATCAATATC TATTAATAGT AACTTGGCAA | ||
+ | 781 GTACATTACT ATTGGAAGTA CGCCAGGGTA CATTGGCAGT ACTCCCATTG ACGTCAATGG | ||
+ | 841 CGGTAAATGG CCCGCGATGG CTGCCAAGTA CATCCCCATT GACGTCAATG GGGAGGGGCA | ||
+ | 901 ATGACGCAAA TGGGCGTTCC ATTGACGTAA ATGGGCGGTA GGCGTGCCTA ATGGGAGGTC | ||
+ | 961 TATATAAGCA ATGCTCGTTT AGGGAACCGC CATTCTGCCT GGGGACGTCG GAGCAAGCTT | ||
+ | 1021 GATTTAGGTG ACACTATAGA ATACAAGCTA CTTGTTCTTT TTGCAGGATC CGGCCGGCCA | ||
+ | 1081 CCATGGAGGA AGTATGTGAT TCATCAGCAG CTGCGAGCAG CACAGTCCAA AATCAGCCAC | ||
+ | 1141 AGGATCAAGA GCACCCGTGG CCGTATCTTC GCGAATTCTT TTCTTTAAGT GGTGTAAATA | ||
+ | 1201 AAGATTCATT CAAGATGAAA TGTGTCCTCT GTCTCCCGCT TAATAAAGAA ATATCGGCCT | ||
+ | 1261 TCAAAAGTTC GCCATCAAAC CTAAGGAAGC ATATTGAGAG AATGCACCCA AATTACCTCA | ||
+ | 1321 AAAACTACTC TAAATTGACA GCACAGAAGA GAAAGATCGG GACCTCCACC CATGCTTCCA | ||
+ | 1381 GCAGTAAGCA ACTGAAAGTT GACTCAGTTT TCCCAGTCAA ACATGTGTCT CCAGTCACTG | ||
+ | 1441 TGAACAAAGC TATATTAAGG TACATCATTC AAGGACTTCA TCCTTTCAGC ACTGTTGATC | ||
+ | 1501 TGCCATCATT TAAAGAGCTG ATTAGTACAC TGCAGCCTGG CATTTCTGTC ATTACAAGGC | ||
+ | 1561 CTACTTTACG CTCCAAGATA GCTGAAGCTG CTCTGATCAT GAAACAGAAA GTGACTGCTG | ||
+ | 1621 CCATGAGTGA AGTTGAATGG ATTGCAACCA CAACGGATTG TTGGACTGCA CGTAGAAAGT | ||
+ | 1681 CATTCATTGG TGTAACTGCT CACTGGATCA ACCCTGGAAG TCTTGAAAGA CATTCCGCTG | ||
+ | 1741 CACTTGCCTG CAAAAGATTA ATGGGCTCTC ATACTTTTGA GGTACTGGCC AGTGCCATGA | ||
+ | 1801 ATGATATCCA CTCAGAGTAT GAAATACGTG ACAAGGTTGT TTGCACAACC ACAGACAGTG | ||
+ | 1861 GTTCCAACTT TATGAAGGCT TTCAGAGTTT TTGGTGTGGA AAACAATGAT ATCGAGACTG | ||
+ | 1921 AGGCAAGAAG GTGTGAAAGT GATGACACTG ATTCTGAAGG CTGTGGTGAG GGAAGTGATG | ||
+ | 1981 GTGTGGAATT CCAAGATGCC TCACGAGTCC TGGACCAAGA CGATGGCTTC GAATTCCAGC | ||
+ | 2041 TACCAAAACA TCAAAAGTGT GCCTGTCACT TACTTAACCT AGTCTCAAGC GTTGATGCCC | ||
+ | 2101 AAAAAGCTCT CTCAAATGAA CACTACAAGA AACTCTACAG ATCTGTCTTT GGCAAATGCC | ||
+ | 2161 AAGCTTTATG GAATAAAAGC AGCCGATCGG CTCTAGCAGC TGAAGCTGTT GAATCAGAAA | ||
+ | 2221 GCCGGCTTCA GCTTTTAAGG CCAAACCAAA CGCGGTGGAA TTCAACTTTT ATGGCTGTTG | ||
+ | 2281 ACAGAATTCT TCAAATTTGC AAAGAAGCAG GAGAAGGCGC ACTTCGGAAT ATATGCACCT | ||
+ | 2341 CTCTTGAGGT TCCAATGTTT AATCCAGCAG AAATGCTGTT CTTGACAGAG TGGGCCAACA | ||
+ | 2401 CAATGCGTCC AGTTGCAAAA GTACTCGACA TCTTGCAAGC GGAAACGAAT ACACAGCTGG | ||
+ | 2461 GGTGGCTGCT GCCTAGTGTC CATCAGTTAA GCTTGAAACT TCAGCGACTC CACCATTCTC | ||
+ | 2521 TCAGGTACTG TGACCCACTT GTGGATGCCC TACAACAAGG AATCCAAACA CGATTCAAGC | ||
+ | 2581 ATATGTTTGA AGATCCTGAG ATCATAGCAG CTGCCATCCT TCTCCCTAAA TTTCGGACCT | ||
+ | 2641 CTTGGACAAA TGATGAAACC ATCATAAAAC GAGGCATGGA CTACATCAGA GTGCATCTGG | ||
+ | 2701 AGCCTTTGGA CCACAAGAAG GAATTGGCCA ACAGTTCATC TGATGATGAA GATTTTTTCG | ||
+ | 2761 CTTCTTTGAA ACCGACAACA CATGAAGCCA GCAAAGAGTT GGATGGATAT CTGGCCTGTG | ||
+ | 2821 TTTCAGACAC CAGGGAGTCT CTGCTCACGT TTCCTGCTAT TTGCAGCCTC TCTATCAAGA | ||
+ | 2881 CTAATACACC TCTTCCCGCA TCGGCTGCCT GTGAGAGGCT TTTCAGCACT GCAGGATTGC | ||
+ | 2941 TTTTCAGCCC CAAAAGAGCT AGGCTTGACA CTAACAATTT TGAGAATCAG CTTCTACTGA | ||
+ | 3001 AGTTAAATCT GAGGTTTTAC AACTTTGAGT AGGGCGCGCC TCTAGAACTA TAGTGAGTCG | ||
+ | 3061 TATTACGTAG ATCCAGACAT GATAAGATAC ATTGATGAGT TTGGACAAAC CACAACTAGA | ||
+ | 3121 ATGCAGTGAA AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT ATTTGTAACC | ||
+ | 3181 ATTATAAGCT GCAATAAACA AGTTAACAAC AACAATTGCA TTCATTTTAT GTTTCAGGTT | ||
+ | 3241 CAGGGGGAGG TGTGGGAGGT TTTTTAATTC GCGGCCGCGG CGCCAATGCA TTGGGCCCGG | ||
+ | 3301 TACCCAGCTT TTGTTCCCTT TAGTGAGGGT TAATTGCGCG CTTGGCGTAA TCATGGTCAT | ||
+ | 3361 AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA | ||
+ | 3421 GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC | ||
+ | 3481 GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC | ||
+ | 3541 AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT | ||
+ | 3601 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC | ||
+ | 3661 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA | ||
+ | 3721 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG | ||
+ | 3781 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA | ||
+ | 3841 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC | ||
+ | 3901 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC | ||
+ | 3961 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC | ||
+ | 4021 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG | ||
+ | 4081 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT | ||
+ | 4141 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA | ||
+ | 4201 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT | ||
+ | 4261 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA | ||
+ | 4321 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG | ||
+ | 4381 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT | ||
+ | 4441 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT | ||
+ | 4501 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC | ||
+ | 4561 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG | ||
+ | 4621 GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG | ||
+ | 4681 ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT | ||
+ | 4741 TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG | ||
+ | 4801 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT | ||
+ | 4861 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA | ||
+ | 4921 TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG | ||
+ | 4981 CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT | ||
+ | 5041 CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA | ||
+ | 5101 TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA | ||
+ | 5161 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT | ||
+ | 5221 TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT | ||
+ | 5281 CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA | ||
+ | 5341 AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT | ||
+ | 5401 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA | ||
+ | 5461 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTAAA TTGTAAGCGT | ||
+ | 5521 TAATATTTTG TTAAAATTCG CGTTAAATTT TTGTTAAATC AGCTCATTTT TTAACCAATA | ||
+ | 5581 GGCCGAAATC GGCAAAATCC CTTATAAATC AAAAGAATAG ACCGAGATAG GGTTGAGTGT | ||
+ | 5641 TGTTCCAGTT TGGAACAAGA GTCCACTATT AAAGAACGTG GACTCCAACG TCAAAGGGCG | ||
+ | 5701 AAAAACCGTC TATCAGGGCG ATGGCCCACT ACGTGAACCA TCACCCTAAT CAAGTTTTTT | ||
+ | 5761 GGGGTCGAGG TGCCGTAAAG CACTAAATCG GAACCCTAAA GGGAGCCCCC GATTTAGAGC | ||
+ | 5821 TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG AAGAAAGCGA AAGGAGCGGG | ||
+ | 5881 CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA ACCACCACAC CCGCCGCGCT | ||
+ | 5941 TAATGCGCCG CTACAGGGCG CGTCCCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG | ||
+ | 6001 GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAG | ||
+ | // | ||
</pre> | </pre> |
Latest revision as of 04:18, 19 December 2007
LOCUS 396.pCS2-transposase 6034 bp ds-DNA circular 18-DEC-2007 REFERENCE 1 AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, Parant JM, Yost HJ, Kanki JP, Chien CB. TITLE The Tol2kit: a multisite gateway-based construction kit for Tol2 transposon transgenesis constructs. JOURNAL Developmental Dynamics 236(11):3088-99 (2007). PUBMED 17937395 COMMENT 396.pCS2-transposase, Tol2kit v1.0 COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers polyA_signal 3070..3267 /label=SV40 late polyA (from pCS2+) /ApEinfo_fwdcolor=#ffff7f /ApEinfo_revcolor=#ffff7f promoter 10..987 /label=sCMV IE94 /ApEinfo_fwdcolor=#ffffbf /ApEinfo_revcolor=#ffffbf rep_origin complement(3699..4372) /label=pUC ori /ApEinfo_fwdcolor=#999999 /ApEinfo_revcolor=#999999 rep_origin complement(3740..4422) /label=colE1 ori /ApEinfo_fwdcolor=#999999 /ApEinfo_revcolor=#999999 rep_origin complement(5640..5946) /label=f1(+) ori /ApEinfo_fwdcolor=#999999 /ApEinfo_revcolor=#999999 CDS complement(4520..5377) /label=ampR /ApEinfo_fwdcolor=#ff7f00 /ApEinfo_revcolor=#ff7f00 primer_bind complement(3351..3372) /label=M13R /ApEinfo_fwdcolor=#777777 /ApEinfo_revcolor=#777777 promoter complement(3316..3335) /label=T3 /ApEinfo_fwdcolor=#777777 /ApEinfo_revcolor=#777777 promoter 1022..1038 /label=SP6 /ApEinfo_fwdcolor=#ffff00 /ApEinfo_revcolor=#ffff00 misc_feature 1083..3032 /label=Tol2 transposase cds /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green ORIGIN 1 TCGACCATAG CCAATTCAAT ATGGCGTATA TGGACTCATG CCAATTCAAT ATGGTGGATC 61 TGGACCTGTG CCAATTCAAT ATGGCGTATA TGGACTCGTG CCAATTCAAT ATGGTGGATC 121 TGGACCCCAG CCAATTCAAT ATGGCGGACT TGGCACCATG CCAATTCAAT ATGGCGGACT 181 TGGCACTGTG CCAACTGGGG AGGGGTCTAC TTGGCACGGT GCCAAGTTTG AGGAGGGGTC 241 TTGGCCCTGT GCCAAGTCCG CCATATTGAA TTGGCATGGT GCCAATAATG GCGGCCATAT 301 TGGCTATATG CCAGGATCAA TATATAGGCA ATATCCAATA TGGCCCTATG CCAATATGGC 361 TATTGGCCAG GTTCAATACT ATGTATTGGC CCTATGCCAT ATAGTATTCC ATATATGGGT 421 TTTCCTATTG ACGTAGATAG CCCCTCCCAA TGGGCGGTCC CATATACCAT ATATGGGGCT 481 TCCTAATACC GCCCATAGCC ACTCCCCCAT TGACGTCAAT GGTCTCTATA TATGGTCTTT 541 CCTATTGACG TCATATGGGC GGTCCTATTG ACGTATATGG CGCCTCCCCC ATTGACGTCA 601 ATTACGGTAA ATGGCCCGCC TGGCTCAATG CCCATTGACG TCAATAGGAC CACCCACCAT 661 TGACGTCAAT GGGATGGCTC ATTGCCCATT CATATCCGTT CTCACGCCCC CTATTGACGT 721 CAATGACGGT AAATGGCCCA CTTGGCAGTA CATCAATATC TATTAATAGT AACTTGGCAA 781 GTACATTACT ATTGGAAGTA CGCCAGGGTA CATTGGCAGT ACTCCCATTG ACGTCAATGG 841 CGGTAAATGG CCCGCGATGG CTGCCAAGTA CATCCCCATT GACGTCAATG GGGAGGGGCA 901 ATGACGCAAA TGGGCGTTCC ATTGACGTAA ATGGGCGGTA GGCGTGCCTA ATGGGAGGTC 961 TATATAAGCA ATGCTCGTTT AGGGAACCGC CATTCTGCCT GGGGACGTCG GAGCAAGCTT 1021 GATTTAGGTG ACACTATAGA ATACAAGCTA CTTGTTCTTT TTGCAGGATC CGGCCGGCCA 1081 CCATGGAGGA AGTATGTGAT TCATCAGCAG CTGCGAGCAG CACAGTCCAA AATCAGCCAC 1141 AGGATCAAGA GCACCCGTGG CCGTATCTTC GCGAATTCTT TTCTTTAAGT GGTGTAAATA 1201 AAGATTCATT CAAGATGAAA TGTGTCCTCT GTCTCCCGCT TAATAAAGAA ATATCGGCCT 1261 TCAAAAGTTC GCCATCAAAC CTAAGGAAGC ATATTGAGAG AATGCACCCA AATTACCTCA 1321 AAAACTACTC TAAATTGACA GCACAGAAGA GAAAGATCGG GACCTCCACC CATGCTTCCA 1381 GCAGTAAGCA ACTGAAAGTT GACTCAGTTT TCCCAGTCAA ACATGTGTCT CCAGTCACTG 1441 TGAACAAAGC TATATTAAGG TACATCATTC AAGGACTTCA TCCTTTCAGC ACTGTTGATC 1501 TGCCATCATT TAAAGAGCTG ATTAGTACAC TGCAGCCTGG CATTTCTGTC ATTACAAGGC 1561 CTACTTTACG CTCCAAGATA GCTGAAGCTG CTCTGATCAT GAAACAGAAA GTGACTGCTG 1621 CCATGAGTGA AGTTGAATGG ATTGCAACCA CAACGGATTG TTGGACTGCA CGTAGAAAGT 1681 CATTCATTGG TGTAACTGCT CACTGGATCA ACCCTGGAAG TCTTGAAAGA CATTCCGCTG 1741 CACTTGCCTG CAAAAGATTA ATGGGCTCTC ATACTTTTGA GGTACTGGCC AGTGCCATGA 1801 ATGATATCCA CTCAGAGTAT GAAATACGTG ACAAGGTTGT TTGCACAACC ACAGACAGTG 1861 GTTCCAACTT TATGAAGGCT TTCAGAGTTT TTGGTGTGGA AAACAATGAT ATCGAGACTG 1921 AGGCAAGAAG GTGTGAAAGT GATGACACTG ATTCTGAAGG CTGTGGTGAG GGAAGTGATG 1981 GTGTGGAATT CCAAGATGCC TCACGAGTCC TGGACCAAGA CGATGGCTTC GAATTCCAGC 2041 TACCAAAACA TCAAAAGTGT GCCTGTCACT TACTTAACCT AGTCTCAAGC GTTGATGCCC 2101 AAAAAGCTCT CTCAAATGAA CACTACAAGA AACTCTACAG ATCTGTCTTT GGCAAATGCC 2161 AAGCTTTATG GAATAAAAGC AGCCGATCGG CTCTAGCAGC TGAAGCTGTT GAATCAGAAA 2221 GCCGGCTTCA GCTTTTAAGG CCAAACCAAA CGCGGTGGAA TTCAACTTTT ATGGCTGTTG 2281 ACAGAATTCT TCAAATTTGC AAAGAAGCAG GAGAAGGCGC ACTTCGGAAT ATATGCACCT 2341 CTCTTGAGGT TCCAATGTTT AATCCAGCAG AAATGCTGTT CTTGACAGAG TGGGCCAACA 2401 CAATGCGTCC AGTTGCAAAA GTACTCGACA TCTTGCAAGC GGAAACGAAT ACACAGCTGG 2461 GGTGGCTGCT GCCTAGTGTC CATCAGTTAA GCTTGAAACT TCAGCGACTC CACCATTCTC 2521 TCAGGTACTG TGACCCACTT GTGGATGCCC TACAACAAGG AATCCAAACA CGATTCAAGC 2581 ATATGTTTGA AGATCCTGAG ATCATAGCAG CTGCCATCCT TCTCCCTAAA TTTCGGACCT 2641 CTTGGACAAA TGATGAAACC ATCATAAAAC GAGGCATGGA CTACATCAGA GTGCATCTGG 2701 AGCCTTTGGA CCACAAGAAG GAATTGGCCA ACAGTTCATC TGATGATGAA GATTTTTTCG 2761 CTTCTTTGAA ACCGACAACA CATGAAGCCA GCAAAGAGTT GGATGGATAT CTGGCCTGTG 2821 TTTCAGACAC CAGGGAGTCT CTGCTCACGT TTCCTGCTAT TTGCAGCCTC TCTATCAAGA 2881 CTAATACACC TCTTCCCGCA TCGGCTGCCT GTGAGAGGCT TTTCAGCACT GCAGGATTGC 2941 TTTTCAGCCC CAAAAGAGCT AGGCTTGACA CTAACAATTT TGAGAATCAG CTTCTACTGA 3001 AGTTAAATCT GAGGTTTTAC AACTTTGAGT AGGGCGCGCC TCTAGAACTA TAGTGAGTCG 3061 TATTACGTAG ATCCAGACAT GATAAGATAC ATTGATGAGT TTGGACAAAC CACAACTAGA 3121 ATGCAGTGAA AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT ATTTGTAACC 3181 ATTATAAGCT GCAATAAACA AGTTAACAAC AACAATTGCA TTCATTTTAT GTTTCAGGTT 3241 CAGGGGGAGG TGTGGGAGGT TTTTTAATTC GCGGCCGCGG CGCCAATGCA TTGGGCCCGG 3301 TACCCAGCTT TTGTTCCCTT TAGTGAGGGT TAATTGCGCG CTTGGCGTAA TCATGGTCAT 3361 AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA 3421 GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC 3481 GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC 3541 AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT 3601 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC 3661 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA 3721 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG 3781 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA 3841 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC 3901 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC 3961 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC 4021 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG 4081 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT 4141 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA 4201 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT 4261 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA 4321 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG 4381 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT 4441 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT 4501 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC 4561 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG 4621 GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG 4681 ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT 4741 TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG 4801 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT 4861 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA 4921 TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG 4981 CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT 5041 CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA 5101 TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA 5161 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT 5221 TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT 5281 CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA 5341 AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT 5401 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA 5461 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTAAA TTGTAAGCGT 5521 TAATATTTTG TTAAAATTCG CGTTAAATTT TTGTTAAATC AGCTCATTTT TTAACCAATA 5581 GGCCGAAATC GGCAAAATCC CTTATAAATC AAAAGAATAG ACCGAGATAG GGTTGAGTGT 5641 TGTTCCAGTT TGGAACAAGA GTCCACTATT AAAGAACGTG GACTCCAACG TCAAAGGGCG 5701 AAAAACCGTC TATCAGGGCG ATGGCCCACT ACGTGAACCA TCACCCTAAT CAAGTTTTTT 5761 GGGGTCGAGG TGCCGTAAAG CACTAAATCG GAACCCTAAA GGGAGCCCCC GATTTAGAGC 5821 TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG AAGAAAGCGA AAGGAGCGGG 5881 CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA ACCACCACAC CCGCCGCGCT 5941 TAATGCGCCG CTACAGGGCG CGTCCCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG 6001 GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAG //