Difference between revisions of "PME-mCherryCAAX Genbank"

Latest revision as of 11:56, 12 October 2009

LOCUS       550.pME-mCherryCAAX       3321 bp ds-DNA     circular     18-DEC-2007
REFERENCE   1
  AUTHORS   Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, 
			Parant JM, Yost HJ, Kanki JP, Chien CB.
  TITLE     The Tol2kit: a multisite gateway-based construction kit for Tol2 
			transposon transgenesis constructs.
  JOURNAL   Developmental Dynamics 236(11):3088-99 (2007).
  PUBMED    17937395
COMMENT     450.pME-mCherryCAAX, Tol2kit v1.2
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_recomb     569..668
                     /label=attL1
                     /ApEinfo_fwdcolor=#3fd0ff
                     /ApEinfo_revcolor=#3fd0ff
     misc_recomb     1446..1545
                     /label=attL2
                     /ApEinfo_fwdcolor=#49a0ff
                     /ApEinfo_revcolor=#49a0ff
     misc_feature    649..662
                     /label=att1_shared
                     /ApEinfo_fwdcolor=#32a6cc
                     /ApEinfo_revcolor=#32a6cc
     misc_feature    1452..1467
                     /label=att2_shared
                     /ApEinfo_fwdcolor=#3a80cc
                     /ApEinfo_revcolor=#3a80cc
     misc_structure  1382..1444
                     /label=CAAX box from Harvey ras
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
     CDS             674..1381
                     /label=mCherry (Svoboda)
                     /ApEinfo_fwdcolor=#dc143c
                     /ApEinfo_revcolor=#dc143c
     rep_origin      2645..3318
                     /label=pUC ori
                     /ApEinfo_fwdcolor=#999999
                     /ApEinfo_revcolor=#999999
     CDS             1715..2524
                     /label=kanR
                     /ApEinfo_fwdcolor=#ff7f3f
                     /ApEinfo_revcolor=#ff7f3f
     primer_bind     complement(1582..1602)
                     /label=M13R
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
     primer_bind     537..552
                     /label=M13F
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
     promoter        complement(1559..1578)
                     /label=T7
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
     terminator      268..295
                     /label=T2
                     /ApEinfo_fwdcolor=#bbbbbb
                     /ApEinfo_revcolor=#bbbbbb
     terminator      427..470
                     /label=T1
                     /ApEinfo_fwdcolor=#bbbbbb
                     /ApEinfo_revcolor=#bbbbbb
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
      601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
      661 AGCAGGCTCC ACCATGGTGA GCAAGGGCGA GGAGGACAAC ATGGCCATCA TCAAGGAGTT
      721 CATGCGCTTC AAGGTGCACA TGGAGGGCTC CGTGAACGGC CACGAGTTCG AGATCGAGGG
      781 CGAGGGCGAG GGCCGCCCCT ACGAGGGCAC CCAGACCGCC AAGCTGAAGG TGACCAAGGG
      841 CGGCCCCCTG CCCTTCGCCT GGGACATCCT GTCCCCTCAG TTCATGTACG GCTCCAAGGC
      901 CTACGTGAAG CACCCCGCCG ACATCCCCGA CTACTTGAAG CTGTCCTTCC CCGAGGGCTT
      961 CAAGTGGGAG CGCGTGATGA ACTTCGAGGA CGGCGGCGTG GTGACCGTGA CCCAGGACTC
     1021 CTCCCTGCAG GACGGCGAGT TCATCTACAA GGTGAAGCTG CGCGGCACCA ACTTCCCCTC
     1081 CGACGGCCCC GTAATGCAGA AGAAGACCAT GGGCTGGGAG GCCTCCTCCG AGCGGATGTA
     1141 CCCCGAGGAC GGCGCCCTGA AGGGCGAGAT CAAGCAGAGG CTGAAGCTGA AGGACGGCGG
     1201 CCACTACGAC GCCGAGGTCA AGACCACCTA CAAGGCCAAG AAGCCCGTGC AGCTGCCCGG
     1261 CGCCTACAAC GTCAACATCA AGCTGGACAT CACCTCCCAC AACGAGGACT ACACCATCGT
     1321 GGAACAGTAC GAGCGCGCCG AGGGCCGCCA CTCCACCGGC GGCATGGACG AGCTGTACAA
     1381 GAAGCTGAAC CCTCCTGATG AGAGTGGCCC CGGCTGCATG AGCTGCAAGT GTGTGCTCTC
     1441 CTGAGACCCA GCTTTCTTGT ACAAAGTTGG CATTATAAGA AAGCATTGCT TATCAATTTG
     1501 TTGCAACGAA CAGGTCACTA TCAGTCAAAA TAAAATCATT ATTTGCCATC CAGCTGATAT
     1561 CCCCTATAGT GAGTCGTATT ACATGGTCAT AGCTGTTTCC TGGCAGCTCT GGCCCGTGTC
     1621 TCAAAATCTC TGATGTTACA TTGCACAAGA TAAAATAATA TCATCATGAA CAATAAAACT
     1681 GTCTGCTTAC ATAAACAGTA ATACAAGGGG TGTTATGAGC CATATTCAAC GGGAAACGTC
     1741 GAGGCCGCGA TTAAATTCCA ACATGGATGC TGATTTATAT GGGTATAAAT GGGCTCGCGA
     1801 TAATGTCGGG CAATCAGGTG CGACAATCTA TCGCTTGTAT GGGAAGCCCG ATGCGCCAGA
     1861 GTTGTTTCTG AAACATGGCA AAGGTAGCGT TGCCAATGAT GTTACAGATG AGATGGTCAG
     1921 ACTAAACTGG CTGACGGAAT TTATGCCTCT TCCGACCATC AAGCATTTTA TCCGTACTCC
     1981 TGATGATGCA TGGTTACTCA CCACTGCGAT CCCCGGAAAA ACAGCATTCC AGGTATTAGA
     2041 AGAATATCCT GATTCAGGTG AAAATATTGT TGATGCGCTG GCAGTGTTCC TGCGCCGGTT
     2101 GCATTCGATT CCTGTTTGTA ATTGTCCTTT TAACAGCGAT CGCGTATTTC GTCTCGCTCA
     2161 GGCGCAATCA CGAATGAATA ACGGTTTGGT TGATGCGAGT GATTTTGATG ACGAGCGTAA
     2221 TGGCTGGCCT GTTGAACAAG TCTGGAAAGA AATGCATAAA CTTTTGCCAT TCTCACCGGA
     2281 TTCAGTCGTC ACTCATGGTG ATTTCTCACT TGATAACCTT ATTTTTGACG AGGGGAAATT
     2341 AATAGGTTGT ATTGATGTTG GACGAGTCGG AATCGCAGAC CGATACCAGG ATCTTGCCAT
     2401 CCTATGGAAC TGCCTCGGTG AGTTTTCTCC TTCATTACAG AAACGGCTTT TTCAAAAATA
     2461 TGGTATTGAT AATCCTGATA TGAATAAATT GCAGTTTCAT TTGATGCTCG ATGAGTTTTT
     2521 CTAATCAGAA TTGGTTAATT GGTTGTAACA CTGGCAGAGC ATTACGCTGA CTTGACGGGA
     2581 CGGCGCAAGC TCATGACCAA AATCCCTTAA CGTGAGTTAC GCGTCGTTCC ACTGAGCGTC
     2641 AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG
     2701 CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT
     2761 ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT
     2821 TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT
     2881 CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG
     2941 GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC
     3001 GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA
     3061 GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG
     3121 CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA
     3181 TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG
     3241 GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG
     3301 CTGGCCTTTT GCTCACATGT T   
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