From Tol2Kit
(PME-mCherryCAAX sequence H80D moved to PME-mCherryCAAX H80D sequence: indicating H80D mutation in page name) |
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| Line 1: | Line 1: | ||
| − | + | <pre> | |
| + | >pME-mCherryCAAX | ||
| + | CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG | ||
| + | AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA | ||
| + | GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC | ||
| + | GCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGA | ||
| + | AAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAA | ||
| + | GAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTA | ||
| + | GTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGG | ||
| + | CCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCA | ||
| + | GGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCC | ||
| + | GACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGT | ||
| + | TAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCC | ||
| + | AGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGAC | ||
| + | CTGTTCGTTGCAACACATTGATGAGCAATGCTTTTTTATAATGCCAACTT | ||
| + | TGTACAAAAAAGCAGGCTCCACCATGGTGAGCAAGGGCGAGGAGGACAAC | ||
| + | ATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTC | ||
| + | CGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCT | ||
| + | ACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGCGGCCCCCTG | ||
| + | CCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGC | ||
| + | CTACGTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCC | ||
| + | CCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTG | ||
| + | GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAA | ||
| + | GGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCAGA | ||
| + | AGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGAC | ||
| + | GGCGCCCTGAAGGGCGAGATCAAGCAGAGGCTGAAGCTGAAGGACGGCGG | ||
| + | CCACTACGACGCCGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGC | ||
| + | AGCTGCCCGGCGCCTACAACGTCAACATCAAGCTGGACATCACCTCCCAC | ||
| + | AACGAGGACTACACCATCGTGGAACAGTACGAGCGCGCCGAGGGCCGCCA | ||
| + | CTCCACCGGCGGCATGGACGAGCTGTACAAGAAGCTGAACCCTCCTGATG | ||
| + | AGAGTGGCCCCGGCTGCATGAGCTGCAAGTGTGTGCTCTCCTGAGACCCA | ||
| + | GCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTG | ||
| + | TTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATC | ||
| + | CAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCC | ||
| + | TGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGA | ||
| + | TAAAATAATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTA | ||
| + | ATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCGAGGCCGCGA | ||
| + | TTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGA | ||
| + | TAATGTCGGGCAATCAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCG | ||
| + | ATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGAT | ||
| + | GTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCT | ||
| + | TCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCA | ||
| + | CCACTGCGATCCCCGGAAAAACAGCATTCCAGGTATTAGAAGAATATCCT | ||
| + | GATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTT | ||
| + | GCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTC | ||
| + | GTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGT | ||
| + | GATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGA | ||
| + | AATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTG | ||
| + | ATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGT | ||
| + | ATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCAT | ||
| + | CCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTT | ||
| + | TTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCAT | ||
| + | TTGATGCTCGATGAGTTTTTCTAATCAGAATTGGTTAATTGGTTGTAACA | ||
| + | CTGGCAGAGCATTACGCTGACTTGACGGGACGGCGCAAGCTCATGACCAA | ||
| + | AATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTA | ||
| + | GAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTG | ||
| + | CTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGG | ||
| + | ATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCG | ||
| + | CAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTT | ||
| + | CAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTAC | ||
| + | CAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCA | ||
| + | AGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTC | ||
| + | GTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACC | ||
| + | TACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCG | ||
| + | GACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGA | ||
| + | GCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCC | ||
| + | ACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGC | ||
| + | CTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTG | ||
| + | CTGGCCTTTTGCTCACATGTT | ||
| + | >T2 | ||
| + | AAAAGGCCATCCGTCAGGATGGCCTTCT | ||
| + | >T1 | ||
| + | ATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTAT | ||
| + | >attL1 | ||
| + | CAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACACAT | ||
| + | TGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCT | ||
| + | >pME-mCherryCAAX_insert | ||
| + | TTTGTACAAAAAAGCAGGCTCCACCATGGTGAGCAAGGGCGAGGAGGACA | ||
| + | ACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGC | ||
| + | TCCGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCC | ||
| + | CTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGCGGCCCCC | ||
| + | TGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAG | ||
| + | GCCTACGTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTT | ||
| + | CCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCG | ||
| + | TGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTAC | ||
| + | AAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCA | ||
| + | GAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGG | ||
| + | ACGGCGCCCTGAAGGGCGAGATCAAGCAGAGGCTGAAGCTGAAGGACGGC | ||
| + | GGCCACTACGACGCCGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGT | ||
| + | GCAGCTGCCCGGCGCCTACAACGTCAACATCAAGCTGGACATCACCTCCC | ||
| + | ACAACGAGGACTACACCATCGTGGAACAGTACGAGCGCGCCGAGGGCCGC | ||
| + | CACTCCACCGGCGGCATGGACGAGCTGTACAAGAAGCTGAACCCTCCTGA | ||
| + | TGAGAGTGGCCCCGGCTGCATGAGCTGCAAGTGTGTGCTCTCCTGAGACC | ||
| + | CAGCTTTCTTGTACAAAGT | ||
| + | >mCherry_(no_stop) | ||
| + | ATGGTGAGCAAGGGCGAGGAGGACAACATGGCCATCATCAAGGAGTTCAT | ||
| + | GCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGA | ||
| + | TCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAG | ||
| + | CTGAAGGTGACCAAGGGCGGCCCCCTGCCCTTCGCCTGGGACATCCTGTC | ||
| + | CCCTCAGTTCATGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACA | ||
| + | TCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGC | ||
| + | GTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTC | ||
| + | CCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACT | ||
| + | TCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCC | ||
| + | TCCTCCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGATCAA | ||
| + | GCAGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCCGAGGTCAAGA | ||
| + | CCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGTC | ||
| + | AACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGA | ||
| + | ACAGTACGAGCGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGC | ||
| + | TGTACAAG | ||
| + | >CAAX_box_from_human_Harvey_Ras | ||
| + | AAGCTGAACCCTCCTGATGAGAGTGGCCCCGGCTGCATGAGCTGCAAGTG | ||
| + | TGTGCTCTCCTGA | ||
| + | >attL2 | ||
| + | ACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCA | ||
| + | ATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTG | ||
| + | >T7 | ||
| + | TACGACTCACTATAGGGGAT | ||
| + | >M13_Reverse | ||
| + | CAGGAAACAGCTATGAC | ||
| + | >kan | ||
| + | ATGAGCCATATTCAACGGGAAACGTCGAGGCCGCGATTAAATTCCAACAT | ||
| + | GGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAAT | ||
| + | CAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTTG | ||
| + | TTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGAT | ||
| + | GGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGC | ||
| + | ATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCC | ||
| + | GGAAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAA | ||
| + | TATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTG | ||
| + | TTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCG | ||
| + | CAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGA | ||
| + | GCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTT | ||
| + | TGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGAT | ||
| + | AACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACG | ||
| + | AGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCC | ||
| + | TCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGT | ||
| + | ATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGA | ||
| + | GTTTTTCTAA | ||
| + | >pUC_ori | ||
| + | CCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGT | ||
| + | AATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTT | ||
| + | TGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGC | ||
| + | AGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCA | ||
| + | CCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCC | ||
| + | TGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTG | ||
| + | GACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGG | ||
| + | GGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGA | ||
| + | GATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGA | ||
| + | AAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCAC | ||
| + | GAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGT | ||
| + | TTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGG | ||
| + | CGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGC | ||
| + | CTTTTGCTGGCCTTTTGCTCACAT | ||
| + | </pre> | ||
Latest revision as of 23:09, 2 November 2007
>pME-mCherryCAAX CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGA AAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAA GAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTA GTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGG CCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCA GGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCC GACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGT TAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCC AGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGAC CTGTTCGTTGCAACACATTGATGAGCAATGCTTTTTTATAATGCCAACTT TGTACAAAAAAGCAGGCTCCACCATGGTGAGCAAGGGCGAGGAGGACAAC ATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTC CGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCT ACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGCGGCCCCCTG CCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGC CTACGTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCC CCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTG GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAA GGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCAGA AGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGAC GGCGCCCTGAAGGGCGAGATCAAGCAGAGGCTGAAGCTGAAGGACGGCGG CCACTACGACGCCGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGC AGCTGCCCGGCGCCTACAACGTCAACATCAAGCTGGACATCACCTCCCAC AACGAGGACTACACCATCGTGGAACAGTACGAGCGCGCCGAGGGCCGCCA CTCCACCGGCGGCATGGACGAGCTGTACAAGAAGCTGAACCCTCCTGATG AGAGTGGCCCCGGCTGCATGAGCTGCAAGTGTGTGCTCTCCTGAGACCCA GCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTG TTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATC CAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCC TGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGA TAAAATAATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTA ATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCGAGGCCGCGA TTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGA TAATGTCGGGCAATCAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCG ATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGAT GTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCT TCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCA CCACTGCGATCCCCGGAAAAACAGCATTCCAGGTATTAGAAGAATATCCT GATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTT GCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTC GTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGT GATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGA AATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTG ATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGT ATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCAT CCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTT TTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCAT TTGATGCTCGATGAGTTTTTCTAATCAGAATTGGTTAATTGGTTGTAACA CTGGCAGAGCATTACGCTGACTTGACGGGACGGCGCAAGCTCATGACCAA AATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTA GAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTG CTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGG ATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCG CAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTT CAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTAC CAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCA AGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTC GTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACC TACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCG GACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGA GCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCC ACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGC CTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTG CTGGCCTTTTGCTCACATGTT >T2 AAAAGGCCATCCGTCAGGATGGCCTTCT >T1 ATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTAT >attL1 CAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACACAT TGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCT >pME-mCherryCAAX_insert TTTGTACAAAAAAGCAGGCTCCACCATGGTGAGCAAGGGCGAGGAGGACA ACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGC TCCGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCC CTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGCGGCCCCC TGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAG GCCTACGTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTT CCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCG TGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTAC AAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCA GAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGG ACGGCGCCCTGAAGGGCGAGATCAAGCAGAGGCTGAAGCTGAAGGACGGC GGCCACTACGACGCCGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGT GCAGCTGCCCGGCGCCTACAACGTCAACATCAAGCTGGACATCACCTCCC ACAACGAGGACTACACCATCGTGGAACAGTACGAGCGCGCCGAGGGCCGC CACTCCACCGGCGGCATGGACGAGCTGTACAAGAAGCTGAACCCTCCTGA TGAGAGTGGCCCCGGCTGCATGAGCTGCAAGTGTGTGCTCTCCTGAGACC CAGCTTTCTTGTACAAAGT >mCherry_(no_stop) ATGGTGAGCAAGGGCGAGGAGGACAACATGGCCATCATCAAGGAGTTCAT GCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGA TCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAG CTGAAGGTGACCAAGGGCGGCCCCCTGCCCTTCGCCTGGGACATCCTGTC CCCTCAGTTCATGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACA TCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGC GTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTC CCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACT TCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCC TCCTCCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGATCAA GCAGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCCGAGGTCAAGA CCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGTC AACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGA ACAGTACGAGCGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGC TGTACAAG >CAAX_box_from_human_Harvey_Ras AAGCTGAACCCTCCTGATGAGAGTGGCCCCGGCTGCATGAGCTGCAAGTG TGTGCTCTCCTGA >attL2 ACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCA ATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTG >T7 TACGACTCACTATAGGGGAT >M13_Reverse CAGGAAACAGCTATGAC >kan ATGAGCCATATTCAACGGGAAACGTCGAGGCCGCGATTAAATTCCAACAT GGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAAT CAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTTG TTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGAT GGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGC ATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCC GGAAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAA TATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTG TTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCG CAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGA GCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTT TGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGAT AACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACG AGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCC TCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGT ATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGA GTTTTTCTAA >pUC_ori CCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGT AATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTT TGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGC AGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCA CCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCC TGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTG GACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGG GGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGA GATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGA AAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCAC GAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGT TTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGG CGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGC CTTTTGCTGGCCTTTTGCTCACAT