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(New page: '''Construction details'''<br> p5E-MCS was made by PCR of the pBSII SK+ multiple cloning site, from T7 to T3 primers, followed by a BP reaction. Sequencing confirms that all bases of the i...)
 
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'''Construction details'''<br>
+
=== Construction details ===
 
p5E-MCS was made by PCR of the pBSII SK+ multiple cloning site, from T7 to T3 primers, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct.
 
p5E-MCS was made by PCR of the pBSII SK+ multiple cloning site, from T7 to T3 primers, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct.
  
'''Crudely annotated sequence'''<br>
+
=== Crudely annotated sequence ===
 
FASTA file with the full-length sequence as well as sequences of individual components:<br>
 
FASTA file with the full-length sequence as well as sequences of individual components:<br>
 
[[p5E-MCS sequence]]
 
[[p5E-MCS sequence]]
  
'''Crude map'''<br>
+
=== Crude map ===
 
Screenshot from Sequencher showing locations of components:<br>
 
Screenshot from Sequencher showing locations of components:<br>
 
[[Image:p5E-MCS.png]]
 
[[Image:p5E-MCS.png]]

Revision as of 22:55, 19 March 2007

Construction details

p5E-MCS was made by PCR of the pBSII SK+ multiple cloning site, from T7 to T3 primers, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct.

Crudely annotated sequence

FASTA file with the full-length sequence as well as sequences of individual components:
p5E-MCS sequence

Crude map

Screenshot from Sequencher showing locations of components:
P5E-MCS.png