From Tol2Kit
(New page: <pre> >pDestTol2CG2 CACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAA TACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTT CAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGC CCTTATTCCCTTTTT...) |
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<pre> | <pre> | ||
>pDestTol2CG2 | >pDestTol2CG2 | ||
| − | + | CCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTT | |
| − | + | TGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCC | |
| − | + | TTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTT | |
| − | + | GGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGA | |
| − | + | AAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATC | |
| − | + | AAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAG | |
| − | + | GGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGGCTGGCTAAGAACT | |
| − | + | CATCAGCCTCCCCGGTCCATCTACCCACGTACCAATGCACCAATTGGCCA | |
| − | + | CAATGACGGCTACTACATGGTGCCATTCCTTCCTCTTTATAGGAATGGAG | |
| − | + | ACTACCTCCTGTCCAACAAGGCTCTTGGATACGAGTACGCCTACCTGTTG | |
| − | + | GACCCAGGTCATTGCACAACACCAGAAATGCCCTCTGATCTGCAAAAGAC | |
| − | + | GTGAATATCTGTTCAGACACCCATATCCACTCTGTTCCACACAGGTCAGA | |
| − | + | GGTTTGTCCAGGAGTTCTTGACAGAGGTGTAAAAAGTACTCAAAAATTTT | |
| − | + | ACTCAAGTGAAAGTACAAGTACTTAGGGAAAATTTTACTCAATTAAAAGT | |
| − | + | AAAAGTATCTGGCTAGAATCTTACTTGAGTAAAAGTAAAAAAGTACTCCA | |
| − | + | TTAAAATTGTACTTGAGTATTAAGGAAGTAAAAGTAAAAGCAAGAAAGAA | |
| − | + | AACTAGAGATTCTTGTTTAAGCTTTTAATCTCAAAAAACATTAAATGAAA | |
| − | + | TGCATACAAGGTTTTATCCTGCTTTAGAACTGTTTGTATTTAATTATCAA | |
| − | + | ACTATAAGACAGACAATCTAATGCCAGTACACGCTACTCAAAGTTGTAAA | |
| − | + | ACCTCAGATTTAACTTCAGTAGAAGCTGATTCTCAAAATTGTTAGTGTCA | |
| − | + | AGCCTAGCTCTTTTGGGGCTGAAAAGCAATCCTGCAGTGCTGAAAAGCCT | |
| − | + | CTCACAGGCAGCCGATGCGGGAAGAGGTGTATTAGTCTTGATAGAGAGGC | |
| − | + | TGCAAATAGCAGGAAACGTGAGCAGAGACTCCCTGGTGTCTGAAACACAG | |
| − | + | GCCAGATGGGCCCTCGAGCAGGAAACAGCTATGACCATGATTACGCCAAG | |
| − | + | CTATCAACTTTGTATAGAAAAGTTGAACGAGAAACGTAAAATGATATAAA | |
| − | + | TATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACT | |
| − | + | GTAAAACACAACATATCCAGTCACTATGGTCGACCTGCAGACTGGCTGTG | |
| − | + | TATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGT | |
| − | + | TTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATA | |
| − | + | ACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTC | |
| − | + | ATCCCCGATATGCACCACCGGGTAAAGTTCACGGGGGACTTTATCTGACA | |
| − | + | GCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCA | |
| − | + | ATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTT | |
| − | + | ATAGGTGTAAACCTTAAACTGCATTTCACCAGCCCCTGTTCTCGTCGGCA | |
| − | + | AAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTG | |
| − | + | ATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCA | |
| − | + | TGGTTGTGCTTACCGAACCGGAGATATTGACATCATATATGCCTTGAGCA | |
| − | + | ACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCA | |
| − | + | TACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATA | |
| − | + | CCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAA | |
| − | + | GCCGGATCCTCTAGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTT | |
| − | + | GTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGA | |
| − | + | TGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATA | |
| − | + | TTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGT | |
| − | + | TTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAAC | |
| − | + | ATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACA | |
| − | + | CGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGT | |
| − | + | ATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTG | |
| − | + | TAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGC | |
| − | + | CATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAA | |
| − | + | AGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCC | |
| − | + | GTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTG | |
| − | + | AAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGG | |
| − | + | TATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAAT | |
| − | + | CTCGACGGATCCTAACTCAAAATCCACACATTATACGAGCCGGAAGCATA | |
| − | + | AAGTGTAAAGCCTGGGGGTGCCTAATGCGGCCGCCATAGTGACTGGATAT | |
| − | + | GTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTT | |
| − | + | AATATATTGATATTTATATCATTTTACGTTTCTCGTTCAACTTTATTATA | |
| − | + | CATAGTTGATAATTCACTGGCCGTCGTTTTACATCGATGATGATCCAGAC | |
| − | + | ATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTG | |
| − | + | AAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAA | |
| − | + | CCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTT | |
| − | + | ATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAA | |
| − | + | CCTCTACAAATGTGGTATGGCTGATTATGATCCTCTAGATCAGATCTCTT | |
| − | + | GTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATT | |
| − | + | TCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAA | |
| − | + | CTCATCAATGTATCTTATCATGTCTGGATCTACGTAATACGACTCACTAT | |
| − | + | AGTTCTAGAGGCTCGAGAGGGGCCGCTTTACTTGTACAGCTCGTCCATGC | |
| − | + | CGAGAGTGATCCCGGCGGCGGTCACGAACTCCAGCAGGACCATGTGATCG | |
| − | + | CGCTTCTCGTTGGGGTCTTTGCTCAGGGCGGACTGGGTGCTCAGGTAGTG | |
| − | + | GTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGTTCTGCTGGT | |
| − | + | AGTGGTCGGCGAGCTGCACGCTGCCGTCCTCGATGTTGTGGCGGATCTTG | |
| − | + | AAGTTCACCTTGATGCCGTTCTTCTGCTTGTCGGCCATGATATAGACGTT | |
| − | + | GTGGCTGTTGTAGTTGTACTCCAGCTTGTGCCCCAGGATGTTGCCGTCCT | |
| − | + | CCTTGAAGTCGATGCCCTTCAGCTCGATGCGGTTCACCAGGGTGTCGCCC | |
| − | + | TCGAACTTCACCTCGGCGCGGGTCTTGTAGTTGCCGTCGTCCTTGAAGAA | |
| − | + | GATGGTGCGCTCCTGGACGTAGCCTTCGGGCATGGCGGACTTGAAGAAGT | |
| − | + | CGTGCTGCTTCATGTGGTCGGGGTAGCGGCTGAAGCACTGCACGCCGTAG | |
| − | + | GTCAGGGTGGTCACGAGGGTGGGCCAGGGCACGGGCAGCTTGCCGGTGGT | |
| − | + | GCAGATGAACTTCAGGGTCAGCTTGCCGTAGGTGGCATCGCCCTCGCCCT | |
| − | + | CGCCGGACACGCTGAACTTGTGGCCGTTTACGTCGCCGTCCAGCTCGACC | |
| − | + | AGGATGGGCACCACCCCGGTGAACAGCTCCTCGCCCTTGCTCACCATGGT | |
| − | + | CACTGTCTGCTTTGCTGTTGGTCTGGGCTCCTGGGTCACTGGCTTACTAA | |
| − | + | TGGAGTCTTTATGTATGAGGACTCTTATCAATTGTTCTTCTATAAAGGTC | |
| − | + | TGCAGTGTTTCTGTTCGTCCCCTACATGGACACCCAGAGCCTCCTAAATA | |
| − | + | CAGGAGCCCTGATAACTGCACAAGTGCTCAGATTCCAGCAGGGTGGAAAA | |
| − | + | TGAGATAAAGTGTGCAGATGGGGAGGGGGACGTGAATGAGAGATTTGAGG | |
| − | + | GATGAAAAGGATGGATGAACGCATTGAAAATAGCCCCTTTCACAAAATAA | |
| − | + | TACCAGTAAATTGTCATATAATTAATGACCAGACCTTTACCTGTAAATGA | |
| − | + | TGTGATGTTCATATACAGAAGAATGCCAACAATTTACAGATGATTTTACA | |
| − | + | AATTCTTAATTTTCCTTTTCTAAATGGATTTGTTCACACATTATCTCTTC | |
| − | + | AAAGCAATTGAATTTTCTGGAAAAGATTGGCTGTGTGTGTAAAAGGTGCT | |
| − | + | ACTGAGGAATTTAATGTGACATGGAATGAAGCAAACAGCAGCCTAAGACA | |
| − | + | GGATGGCAGGAAAAATGTCACATGTTTAAAATAAGAGTGAAACCAACGAC | |
| − | + | CTGGACTTATAGAGACTGTGTGTCCTTAGGCAAAGCTCTTGTACAAACAG | |
| − | + | TGCTTGTGACTGTGAAATTCGGGGTTTGCCTGGATTGTGTTAAATGTGTG | |
| − | + | CTTGGATGTATCATAAAAAAGAATCGGTTCTCTGTATTTGACATTTTCTG | |
| − | + | TAATTTATCTAAAGTTACAACTGGCTATGCCTGATTTTTATCACTATTGA | |
| − | + | ATCAATCATTTTAAAGAATGTTAAGTCTGCTGATTTGGAGAGCTCATTTA | |
| − | + | CATTTATTTTGAATGTCTCTTATTTAACACAACTGATTTAAGCTTTAGAT | |
| − | + | CTGCGAAGATACGGCCACGGGTGCTCTTGATCCTGTGGCTGATTTTGGAC | |
| − | + | TGTGCTGCTCGCAGCTGCTGATGAATCACATACTTCCTCCATTTTCTTCC | |
| − | + | ACTGATTGACTGTTATAATTTCCCTAATTTCCAGGTCAAGGTGCTGTGCA | |
| − | + | TTGTGGTAATAGATGTGACATGACGTCACTTCCAAAGGACCAATGAACAT | |
| − | + | GTCTGACCAATTTCATATAATGTGAAAACGATTTTCATAGGCAGAATAAA | |
| − | + | TAACATTTAAATTAAACTGGGCATCAGCGCAATTCAATTGGTTTGGTAAT | |
| − | + | AGCAAGGGAAAATAGAATGAAGTGATCTCCAAAAAATAAGTACTTTTTGA | |
| − | + | CTGTAAATAAAATTGTAAGGAGTAAAAAGTACTTTTTTTTCTAAAAAAAT | |
| − | + | GTAATTAAGTAAAAGTAAAAGTATTGATTTTTAATTGTACTCAAGTAAAG | |
| − | + | TAAAAATCCCCAAAAATAATACTTAAGTACAGTAATCAAGTAAAATTACT | |
| − | + | CAAGTACTTTACACCTCTGGTTCTTGACCCCCTACCTTCAGCAAGCCCAG | |
| − | + | CAGATCCACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTCCAGCTTT | |
| − | + | TGTTCCCTTTAGTGAGGGTTAATTGCGCGCTTGGCGTAATCATGGTCATA | |
| − | + | GCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATAC | |
| − | + | GAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAA | |
| − | + | CTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCT | |
| − | + | GTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTT | |
| − | + | TGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCG | |
| − | + | GTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACG | |
| − | + | GTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAG | |
| − | + | GCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTC | |
| − | + | CATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCA | |
| − | + | GAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTG | |
| − | + | GAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATAC | |
| − | + | CTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACG | |
| − | + | CTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTG | |
| − | + | TGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTAT | |
| − | + | CGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGC | |
| − | + | CACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGT | |
| − | + | TCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGT | |
| − | + | ATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTC | |
| − | + | TTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCA | |
| − | + | AGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATC | |
| − | + | TTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGAT | |
| − | + | TTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATT | |
| − | + | AAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCT | |
| − | + | GACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCT | |
| − | + | ATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGA | |
| − | + | TACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAC | |
| − | + | CCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAG | |
| − | + | GGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTA | |
| − | + | TTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTG | |
| − | + | CGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTT | |
| − | + | TGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACAT | |
| − | + | GATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATC | |
| − | + | GTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGC | |
| − | + | ACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGA | |
| − | + | CTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCG | |
| − | + | AGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAG | |
| − | + | AACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCT | |
| − | + | CAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCA | |
| − | + | CCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGC | |
| − | + | AAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGA | |
| − | + | AATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTAT | |
| − | + | CAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAA | |
| − | + | TAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTG | |
| + | >pBSII_SK+_5’_of_NgoMI | ||
| + | CCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTT | ||
| + | TGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCC | ||
| + | TTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTT | ||
| + | GGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGA | ||
| + | AAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATC | ||
| + | AAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAG | ||
| + | GGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGG | ||
| + | >f1_(+)_ori_frag | ||
| + | CCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGA | ||
| + | TTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGG | ||
| + | TTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGT | ||
| + | TGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAA | ||
| + | >tyrosinase_genomic_from_AB010101 | ||
| + | GCTGGCTAAGAACTCATCAGCCTCCCCGGTCCATCTACCCACGTACCAAT | ||
| + | GCACCAATTGGCCACAATGACGGCTACTACATGGTGCCATTCCTTCCTCT | ||
| + | TTATAGGAATGGAGACTACCTCCTGTCCAACAAGGCTCTTGGATACGAGT | ||
| + | ACGCCTACCTGTTGGACCCAGGTCATTGCACAACACCAGAAATGCCCTCT | ||
| + | GATCTGCAAAAGACGTGAATATCTGTTCAGACACCCATATCCACTCTGTT | ||
| + | CCACACAGGTCAGAGGTTTGTCCAGGAGTTCTTGAC | ||
| + | >Tyro_frag_ex4-in4-ex5 | ||
| + | CAGGTCATTGCACAACACCAGAAATGCCCTCTGATCTGCAAAAGACGTGA | ||
| + | ATATCTGTTCAGACACCCATATCCACTCTGTTCCACACAGG | ||
| + | >3'_TIR | ||
| + | TACTTTTTACACCTCTG | ||
| + | >Tol2_exon4_136-482 | ||
| + | ATCTGGCCTGTGTTTCAGACACCAGGGAGTCTCTGCTCACGTTTCCTGCT | ||
| + | ATTTGCAGCCTCTCTATCAAGACTAATACACCTCTTCCCGCATCGGCTGC | ||
| + | CTGTGAGAGGCTTTTCAGCACTGCAGGATTGCTTTTCAGCCCCAAAAGAG | ||
| + | CTAGGCTTGACACTAACAATTTTGAGAATCAGCTTCTACTGAAGTTAAAT | ||
| + | CTGAGGTTTTACAACTTTGAGTAGCGTGTACTGGCATTAGATTGTCTGTC | ||
| + | TTATAGTTTGATAATTAAATACAAACAGTTCTAAAGCAGGATAAAACCTT | ||
| + | GTATGCATTTCATTTAATGTTTTTTGAGATTAAAAGCTTAAACAAGA | ||
| + | >M13R | ||
| + | GGAAACAGCTATGACCATGA | ||
| + | >attB4/L4/R4_shared | ||
| + | CAACTTTGTATAGAAAAGTTG | ||
| + | >attR4 | ||
| + | CAACTTTGTATAGAAAAGTTGAACGAGAAACGTAAAATGATATAAATATC | ||
| + | AATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAA | ||
| + | AACACAACATATCCAGTCACTATGG | ||
| + | >ccdB_from_pDEST_R4-R3_(1_mutation) | ||
| + | ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTT | ||
| + | TGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGA | ||
| + | TCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCCCCCGTGAACTT | ||
| + | TACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGA | ||
| + | TATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCA | ||
| + | GCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGA | ||
| + | ATATAA | ||
| + | >ccdB_(original) | ||
| + | TTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGC | ||
| + | GGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTG | ||
| + | GCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCAC | ||
| + | CGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCA | ||
| + | GGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACA | ||
| + | TCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAA | ||
| + | CTGCAT | ||
| + | >cmR | ||
| + | TTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCA | ||
| + | TTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCC | ||
| + | AGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAA | ||
| + | AACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGG | ||
| + | TGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAAC | ||
| + | CCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGA | ||
| + | ATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCG | ||
| + | ATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACA | ||
| + | CTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGG | ||
| + | ATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACT | ||
| + | TGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGA | ||
| + | ACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATG | ||
| + | TTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTT | ||
| + | TTTTCTCCAT | ||
| + | >attR3 | ||
| + | CCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTT | ||
| + | TATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCT | ||
| + | CGTTCAACTTTATTATACATAGTTG | ||
| + | >attB3/L3/R3_shared | ||
| + | CAACTTTATTATACA | ||
| + | >M13_-20 | ||
| + | GTAAAACGACGGCCAGTG | ||
| + | >SV40_late_polyA | ||
| + | GATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAG | ||
| + | AATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTT | ||
| + | TATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGC | ||
| + | ATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAG | ||
| + | CAAGTAAAACCTCTACAAATGTGGTATGGCTGATTAT | ||
| + | >SV40_late_polyA_(short) | ||
| + | GATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAG | ||
| + | AATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTT | ||
| + | TATTTGTAACCATTATAAGCTGCAATAAACAAG | ||
| + | >EGFP | ||
| + | ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGT | ||
| + | CGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGG | ||
| + | GCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACC | ||
| + | ACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTA | ||
| + | CGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACT | ||
| + | TCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTC | ||
| + | TTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGG | ||
| + | CGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGG | ||
| + | ACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAAC | ||
| + | GTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAA | ||
| + | GATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACC | ||
| + | AGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCAC | ||
| + | TACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGA | ||
| + | TCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCA | ||
| + | TGGACGAGCTGTACAAGTAA | ||
| + | >cmlc2_upstream | ||
| + | AAAGCTTAAATCAGTTGTGTTAAATAAGAGACATTCAAAATAAATGTAAA | ||
| + | TGAGCTCTCCAAATCAGCAGACTTAACATTCTTTAAAATGATTGATTCAA | ||
| + | TAGTGATAAAAATCAGGCATAGCCAGTTGTAACTTTAGATAAATTACAGA | ||
| + | AAATGTCAAATACAGAGAACCGATTCTTTTTTATGATACATCCAAGCACA | ||
| + | CATTTAACACAATCCAGGCAAACCCCGAATTTCACAGTCACAAGCACTGT | ||
| + | TTGTACAAGAGCTTTGCCTAAGGACACACAGTCTCTATAAGTCCAGGTCG | ||
| + | TTGGTTTCACTCTTATTTTAAACATGTGACATTTTTCCTGCCATCCTGTC | ||
| + | TTAGGCTGCTGTTTGCTTCATTCCATGTCACATTAAATTCCTCAGTAGCA | ||
| + | CCTTTTACACACACAGCCAATCTTTTCCAGAAAATTCAATTGCTTTGAAG | ||
| + | AGATAATGTGTGAACAAATCCATTTAGAAAAGGAAAATTAAGAATTTGTA | ||
| + | AAATCATCTGTAAATTGTTGGCATTCTTCTGTATATGAACATCACATCAT | ||
| + | TTACAGGTAAAGGTCTGGTCATTAATTATATGACAATTTACTGGTATTAT | ||
| + | TTTGTGAAAGGGGCTATTTTCAATGCGTTCATCCATCCTTTTCATCCCTC | ||
| + | AAATCTCTCATTCACGTCCCCCTCCCCATCTGCACACTTTATCTCATTTT | ||
| + | CCACCCTGCTGGAATCTGAGCACTTGTGCAGTTATCAGGGCTCCTGTATT | ||
| + | TAGGAGGCTCTGGGTGTCCATGTAGGGGACGAACAGAAACACTGCAGACC | ||
| + | TTTATAGAAGAACAATTGATAAGAGTCCTCATACATAAAGACTCCATTAG | ||
| + | TAAGCCAGTGACCCAGGAGCCCAGACCAACAGCAAAGCAGACAGTGACC | ||
| + | >Tol2_exon1,_bp1-335_(of_461) | ||
| + | ATTTTTTGGAGATCACTTCATTCTATTTTCCCTTGCTATTACCAAACCAA | ||
| + | TTGAATTGCGCTGATGCCCAGTTTAATTTAAATGTTATTTATTCTGCCTA | ||
| + | TGAAAATCGTTTTCACATTATATGAAATTGGTCAGACATGTTCATTGGTC | ||
| + | CTTTGGAAGTGACGTCATGTCACATCTATTACCACAATGCACAGCACCTT | ||
| + | GACCTGGAAATTAGGGAAATTATAACAGTCAATCAGTGGAAGAAAATGGA | ||
| + | GGAAGTATGTGATTCATCAGCAGCTGCGAGCAGCACAGTCCAAAATCAGC | ||
| + | CACAGGATCAAGAGCACCCGTGGCCGTATCTTCGC | ||
| + | >5'_TIR | ||
| + | CAGAGGTGTAAAGTACTTG | ||
| + | >Tyro_frag_ex5 | ||
| + | GATCTGCTGGGCTTGCTGAAGGTAGGGGGTCAAGAAC | ||
>pBSII_SK+_3’_of_SpeI | >pBSII_SK+_3’_of_SpeI | ||
ACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTCCAGCTTTTGTTCCC | ACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTCCAGCTTTTGTTCCC | ||
| Line 203: | Line 340: | ||
ATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAA | ATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAA | ||
ATAGGGGTTCCGCGCACATTTCCCCGAAAAGTG | ATAGGGGTTCCGCGCACATTTCCCCGAAAAGTG | ||
| + | >T3_primer | ||
| + | AATTAACCCTCACTAAAGGG | ||
| + | >M13R | ||
| + | GGAAACAGCTATGACCATGA | ||
| + | >pUC_ori | ||
| + | TGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCT | ||
| + | GGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGAC | ||
| + | GCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCG | ||
| + | TTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCT | ||
| + | TACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC | ||
| + | ATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAG | ||
| + | CTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATC | ||
| + | CGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCAC | ||
| + | TGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGT | ||
| + | GCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGAC | ||
| + | AGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAG | ||
| + | TTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTT | ||
| + | TTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGA | ||
| + | TCCTTTGATCTTTTCTAC | ||
>amp | >amp | ||
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATT | ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATT | ||
| Line 222: | Line 378: | ||
TATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTA | TATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTA | ||
AGCATTGG | AGCATTGG | ||
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</pre> | </pre> | ||
Revision as of 23:05, 19 March 2007
>pDestTol2CG2 CCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTT TGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCC TTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTT GGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGA AAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATC AAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAG GGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGGCTGGCTAAGAACT CATCAGCCTCCCCGGTCCATCTACCCACGTACCAATGCACCAATTGGCCA CAATGACGGCTACTACATGGTGCCATTCCTTCCTCTTTATAGGAATGGAG ACTACCTCCTGTCCAACAAGGCTCTTGGATACGAGTACGCCTACCTGTTG GACCCAGGTCATTGCACAACACCAGAAATGCCCTCTGATCTGCAAAAGAC GTGAATATCTGTTCAGACACCCATATCCACTCTGTTCCACACAGGTCAGA GGTTTGTCCAGGAGTTCTTGACAGAGGTGTAAAAAGTACTCAAAAATTTT ACTCAAGTGAAAGTACAAGTACTTAGGGAAAATTTTACTCAATTAAAAGT AAAAGTATCTGGCTAGAATCTTACTTGAGTAAAAGTAAAAAAGTACTCCA TTAAAATTGTACTTGAGTATTAAGGAAGTAAAAGTAAAAGCAAGAAAGAA AACTAGAGATTCTTGTTTAAGCTTTTAATCTCAAAAAACATTAAATGAAA TGCATACAAGGTTTTATCCTGCTTTAGAACTGTTTGTATTTAATTATCAA ACTATAAGACAGACAATCTAATGCCAGTACACGCTACTCAAAGTTGTAAA ACCTCAGATTTAACTTCAGTAGAAGCTGATTCTCAAAATTGTTAGTGTCA AGCCTAGCTCTTTTGGGGCTGAAAAGCAATCCTGCAGTGCTGAAAAGCCT CTCACAGGCAGCCGATGCGGGAAGAGGTGTATTAGTCTTGATAGAGAGGC TGCAAATAGCAGGAAACGTGAGCAGAGACTCCCTGGTGTCTGAAACACAG GCCAGATGGGCCCTCGAGCAGGAAACAGCTATGACCATGATTACGCCAAG CTATCAACTTTGTATAGAAAAGTTGAACGAGAAACGTAAAATGATATAAA TATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACT GTAAAACACAACATATCCAGTCACTATGGTCGACCTGCAGACTGGCTGTG TATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGT TTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATA ACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTC ATCCCCGATATGCACCACCGGGTAAAGTTCACGGGGGACTTTATCTGACA GCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCA ATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTT ATAGGTGTAAACCTTAAACTGCATTTCACCAGCCCCTGTTCTCGTCGGCA AAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTG ATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCA TGGTTGTGCTTACCGAACCGGAGATATTGACATCATATATGCCTTGAGCA ACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCA TACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATA CCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAA GCCGGATCCTCTAGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTT GTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGA TGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATA TTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGT TTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAAC ATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACA CGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGT ATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTG TAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGC CATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAA AGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCC GTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTG AAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGG TATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAAT CTCGACGGATCCTAACTCAAAATCCACACATTATACGAGCCGGAAGCATA AAGTGTAAAGCCTGGGGGTGCCTAATGCGGCCGCCATAGTGACTGGATAT GTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTT AATATATTGATATTTATATCATTTTACGTTTCTCGTTCAACTTTATTATA CATAGTTGATAATTCACTGGCCGTCGTTTTACATCGATGATGATCCAGAC ATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTG AAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAA CCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTT ATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAA CCTCTACAAATGTGGTATGGCTGATTATGATCCTCTAGATCAGATCTCTT GTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATT TCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAA CTCATCAATGTATCTTATCATGTCTGGATCTACGTAATACGACTCACTAT AGTTCTAGAGGCTCGAGAGGGGCCGCTTTACTTGTACAGCTCGTCCATGC CGAGAGTGATCCCGGCGGCGGTCACGAACTCCAGCAGGACCATGTGATCG CGCTTCTCGTTGGGGTCTTTGCTCAGGGCGGACTGGGTGCTCAGGTAGTG GTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGTTCTGCTGGT AGTGGTCGGCGAGCTGCACGCTGCCGTCCTCGATGTTGTGGCGGATCTTG AAGTTCACCTTGATGCCGTTCTTCTGCTTGTCGGCCATGATATAGACGTT GTGGCTGTTGTAGTTGTACTCCAGCTTGTGCCCCAGGATGTTGCCGTCCT CCTTGAAGTCGATGCCCTTCAGCTCGATGCGGTTCACCAGGGTGTCGCCC TCGAACTTCACCTCGGCGCGGGTCTTGTAGTTGCCGTCGTCCTTGAAGAA GATGGTGCGCTCCTGGACGTAGCCTTCGGGCATGGCGGACTTGAAGAAGT CGTGCTGCTTCATGTGGTCGGGGTAGCGGCTGAAGCACTGCACGCCGTAG GTCAGGGTGGTCACGAGGGTGGGCCAGGGCACGGGCAGCTTGCCGGTGGT GCAGATGAACTTCAGGGTCAGCTTGCCGTAGGTGGCATCGCCCTCGCCCT CGCCGGACACGCTGAACTTGTGGCCGTTTACGTCGCCGTCCAGCTCGACC AGGATGGGCACCACCCCGGTGAACAGCTCCTCGCCCTTGCTCACCATGGT CACTGTCTGCTTTGCTGTTGGTCTGGGCTCCTGGGTCACTGGCTTACTAA TGGAGTCTTTATGTATGAGGACTCTTATCAATTGTTCTTCTATAAAGGTC TGCAGTGTTTCTGTTCGTCCCCTACATGGACACCCAGAGCCTCCTAAATA CAGGAGCCCTGATAACTGCACAAGTGCTCAGATTCCAGCAGGGTGGAAAA TGAGATAAAGTGTGCAGATGGGGAGGGGGACGTGAATGAGAGATTTGAGG GATGAAAAGGATGGATGAACGCATTGAAAATAGCCCCTTTCACAAAATAA TACCAGTAAATTGTCATATAATTAATGACCAGACCTTTACCTGTAAATGA TGTGATGTTCATATACAGAAGAATGCCAACAATTTACAGATGATTTTACA AATTCTTAATTTTCCTTTTCTAAATGGATTTGTTCACACATTATCTCTTC AAAGCAATTGAATTTTCTGGAAAAGATTGGCTGTGTGTGTAAAAGGTGCT ACTGAGGAATTTAATGTGACATGGAATGAAGCAAACAGCAGCCTAAGACA GGATGGCAGGAAAAATGTCACATGTTTAAAATAAGAGTGAAACCAACGAC CTGGACTTATAGAGACTGTGTGTCCTTAGGCAAAGCTCTTGTACAAACAG TGCTTGTGACTGTGAAATTCGGGGTTTGCCTGGATTGTGTTAAATGTGTG CTTGGATGTATCATAAAAAAGAATCGGTTCTCTGTATTTGACATTTTCTG TAATTTATCTAAAGTTACAACTGGCTATGCCTGATTTTTATCACTATTGA ATCAATCATTTTAAAGAATGTTAAGTCTGCTGATTTGGAGAGCTCATTTA CATTTATTTTGAATGTCTCTTATTTAACACAACTGATTTAAGCTTTAGAT CTGCGAAGATACGGCCACGGGTGCTCTTGATCCTGTGGCTGATTTTGGAC TGTGCTGCTCGCAGCTGCTGATGAATCACATACTTCCTCCATTTTCTTCC ACTGATTGACTGTTATAATTTCCCTAATTTCCAGGTCAAGGTGCTGTGCA TTGTGGTAATAGATGTGACATGACGTCACTTCCAAAGGACCAATGAACAT GTCTGACCAATTTCATATAATGTGAAAACGATTTTCATAGGCAGAATAAA TAACATTTAAATTAAACTGGGCATCAGCGCAATTCAATTGGTTTGGTAAT AGCAAGGGAAAATAGAATGAAGTGATCTCCAAAAAATAAGTACTTTTTGA CTGTAAATAAAATTGTAAGGAGTAAAAAGTACTTTTTTTTCTAAAAAAAT GTAATTAAGTAAAAGTAAAAGTATTGATTTTTAATTGTACTCAAGTAAAG TAAAAATCCCCAAAAATAATACTTAAGTACAGTAATCAAGTAAAATTACT CAAGTACTTTACACCTCTGGTTCTTGACCCCCTACCTTCAGCAAGCCCAG CAGATCCACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTCCAGCTTT TGTTCCCTTTAGTGAGGGTTAATTGCGCGCTTGGCGTAATCATGGTCATA GCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATAC GAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAA CTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCT GTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTT TGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCG GTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACG GTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAG GCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTC CATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCA GAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTG GAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATAC CTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACG CTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTG TGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTAT CGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGC CACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGT TCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGT ATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTC TTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCA AGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATC TTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGAT TTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATT AAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCT GACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCT ATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGA TACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAC CCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAG GGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTA TTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTG CGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTT TGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACAT GATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATC GTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGC ACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGA CTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCG AGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAG AACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCT CAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCA CCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGC AAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGA AATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTAT CAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAA TAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTG >pBSII_SK+_5’_of_NgoMI CCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTT TGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCC TTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTT GGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGA AAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATC AAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAG GGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGG >f1_(+)_ori_frag CCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGA TTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGG TTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGT TGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAA >tyrosinase_genomic_from_AB010101 GCTGGCTAAGAACTCATCAGCCTCCCCGGTCCATCTACCCACGTACCAAT GCACCAATTGGCCACAATGACGGCTACTACATGGTGCCATTCCTTCCTCT TTATAGGAATGGAGACTACCTCCTGTCCAACAAGGCTCTTGGATACGAGT ACGCCTACCTGTTGGACCCAGGTCATTGCACAACACCAGAAATGCCCTCT GATCTGCAAAAGACGTGAATATCTGTTCAGACACCCATATCCACTCTGTT CCACACAGGTCAGAGGTTTGTCCAGGAGTTCTTGAC >Tyro_frag_ex4-in4-ex5 CAGGTCATTGCACAACACCAGAAATGCCCTCTGATCTGCAAAAGACGTGA ATATCTGTTCAGACACCCATATCCACTCTGTTCCACACAGG >3'_TIR TACTTTTTACACCTCTG >Tol2_exon4_136-482 ATCTGGCCTGTGTTTCAGACACCAGGGAGTCTCTGCTCACGTTTCCTGCT ATTTGCAGCCTCTCTATCAAGACTAATACACCTCTTCCCGCATCGGCTGC CTGTGAGAGGCTTTTCAGCACTGCAGGATTGCTTTTCAGCCCCAAAAGAG CTAGGCTTGACACTAACAATTTTGAGAATCAGCTTCTACTGAAGTTAAAT CTGAGGTTTTACAACTTTGAGTAGCGTGTACTGGCATTAGATTGTCTGTC TTATAGTTTGATAATTAAATACAAACAGTTCTAAAGCAGGATAAAACCTT GTATGCATTTCATTTAATGTTTTTTGAGATTAAAAGCTTAAACAAGA >M13R GGAAACAGCTATGACCATGA >attB4/L4/R4_shared CAACTTTGTATAGAAAAGTTG >attR4 CAACTTTGTATAGAAAAGTTGAACGAGAAACGTAAAATGATATAAATATC AATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAA AACACAACATATCCAGTCACTATGG >ccdB_from_pDEST_R4-R3_(1_mutation) ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTT TGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGA TCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCCCCCGTGAACTT TACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGA TATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCA GCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGA ATATAA >ccdB_(original) TTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGC GGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTG GCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCAC CGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCA GGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACA TCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAA CTGCAT >cmR TTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCA TTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCC AGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAA AACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGG TGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAAC CCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGA ATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCG ATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACA CTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGG ATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACT TGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGA ACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATG TTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTT TTTTCTCCAT >attR3 CCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTT TATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCT CGTTCAACTTTATTATACATAGTTG >attB3/L3/R3_shared CAACTTTATTATACA >M13_-20 GTAAAACGACGGCCAGTG >SV40_late_polyA GATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAG AATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTT TATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGC ATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAG CAAGTAAAACCTCTACAAATGTGGTATGGCTGATTAT >SV40_late_polyA_(short) GATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAG AATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTT TATTTGTAACCATTATAAGCTGCAATAAACAAG >EGFP ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGT CGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGG GCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACC ACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTA CGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACT TCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTC TTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGG CGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGG ACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAAC GTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAA GATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACC AGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCAC TACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGA TCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCA TGGACGAGCTGTACAAGTAA >cmlc2_upstream AAAGCTTAAATCAGTTGTGTTAAATAAGAGACATTCAAAATAAATGTAAA TGAGCTCTCCAAATCAGCAGACTTAACATTCTTTAAAATGATTGATTCAA TAGTGATAAAAATCAGGCATAGCCAGTTGTAACTTTAGATAAATTACAGA AAATGTCAAATACAGAGAACCGATTCTTTTTTATGATACATCCAAGCACA CATTTAACACAATCCAGGCAAACCCCGAATTTCACAGTCACAAGCACTGT TTGTACAAGAGCTTTGCCTAAGGACACACAGTCTCTATAAGTCCAGGTCG TTGGTTTCACTCTTATTTTAAACATGTGACATTTTTCCTGCCATCCTGTC TTAGGCTGCTGTTTGCTTCATTCCATGTCACATTAAATTCCTCAGTAGCA CCTTTTACACACACAGCCAATCTTTTCCAGAAAATTCAATTGCTTTGAAG AGATAATGTGTGAACAAATCCATTTAGAAAAGGAAAATTAAGAATTTGTA AAATCATCTGTAAATTGTTGGCATTCTTCTGTATATGAACATCACATCAT TTACAGGTAAAGGTCTGGTCATTAATTATATGACAATTTACTGGTATTAT TTTGTGAAAGGGGCTATTTTCAATGCGTTCATCCATCCTTTTCATCCCTC AAATCTCTCATTCACGTCCCCCTCCCCATCTGCACACTTTATCTCATTTT CCACCCTGCTGGAATCTGAGCACTTGTGCAGTTATCAGGGCTCCTGTATT TAGGAGGCTCTGGGTGTCCATGTAGGGGACGAACAGAAACACTGCAGACC TTTATAGAAGAACAATTGATAAGAGTCCTCATACATAAAGACTCCATTAG TAAGCCAGTGACCCAGGAGCCCAGACCAACAGCAAAGCAGACAGTGACC >Tol2_exon1,_bp1-335_(of_461) ATTTTTTGGAGATCACTTCATTCTATTTTCCCTTGCTATTACCAAACCAA TTGAATTGCGCTGATGCCCAGTTTAATTTAAATGTTATTTATTCTGCCTA TGAAAATCGTTTTCACATTATATGAAATTGGTCAGACATGTTCATTGGTC CTTTGGAAGTGACGTCATGTCACATCTATTACCACAATGCACAGCACCTT GACCTGGAAATTAGGGAAATTATAACAGTCAATCAGTGGAAGAAAATGGA GGAAGTATGTGATTCATCAGCAGCTGCGAGCAGCACAGTCCAAAATCAGC CACAGGATCAAGAGCACCCGTGGCCGTATCTTCGC >5'_TIR CAGAGGTGTAAAGTACTTG >Tyro_frag_ex5 GATCTGCTGGGCTTGCTGAAGGTAGGGGGTCAAGAAC >pBSII_SK+_3’_of_SpeI ACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTCCAGCTTTTGTTCCC TTTAGTGAGGGTTAATTGCGCGCTTGGCGTAATCATGGTCATAGCTGTTT CCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGG AAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACAT TAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGC CAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTAT TGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTC GGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCC ACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCA AAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGG CGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTC CCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCG CCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGG TATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGA ACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGT AACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAA GTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCG CTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCC GGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCA GATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTA CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTC ATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATG AAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTT ACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGT TCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGA GGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCT CACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAG CGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTG TTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACG TTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATG GCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCC CATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCA GAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCAT AATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGA GTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCT CTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTA AAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGAT CTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACT GATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACA GGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTG AATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTT ATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAA ATAGGGGTTCCGCGCACATTTCCCCGAAAAGTG >T3_primer AATTAACCCTCACTAAAGGG >M13R GGAAACAGCTATGACCATGA >pUC_ori TGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCT GGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGAC GCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCG TTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCT TACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC ATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAG CTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATC CGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCAC TGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGT GCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGAC AGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAG TTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTT TTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGA TCCTTTGATCTTTTCTAC >amp ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATT TTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATG CTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGAT GAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACG CCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGT AAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCA ACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCT GAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAA TGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACC ACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTG GAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAC TATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTA AGCATTGG