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Revision as of 01:45, 24 March 2007 by Chi-bin (talk | contribs) (New page: <pre> >pME-EGFP-CAAX CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTC...)
>pME-EGFP-CAAX CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGA AAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAA GAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTA GTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGG CCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCA GGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCC GACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGT TAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCC AGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGAC CTGTTCGTTGCAACACATTGATGAGCAATGCTTTTTTATAATGCCAACTT TGTACAAAAAAGCAGGCTGGACCATGGTGAGCAAGGGCGAGGAGCTGTTC ACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCA CAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGC TGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCC ACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCC CGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCT ACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACC CGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCT GAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGG AGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAG AACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAG CGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCC CCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGC AAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGAC CGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGGGAGGAGGAA GATCTAAGCTGAACCCTCCTGATGAGAGTGGCCCCGGCTGCATGAGCTGC AAGTGTGTGCTCTCCTGAGACCCAGCTTTCTTGTACAAAGTTGGCATTAT AAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTC AAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCG TATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAA TCTCTGATGTTACATTGCACAAGATAAAATAATATCATCATGAACAATAA AACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATT CAACGGGAAACGTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTT ATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAA TCTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACAT GGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAA CTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTA CTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGAAAAACAGCA TTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGC GCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTC CTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATG AATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTG GCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCAC CGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTT GACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGC AGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTT CTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCT GATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATC AGAATTGGTTAATTGGTTGTAACACTGGCAGAGCATTACGCTGACTTGAC GGGACGGCGCAAGCTCATGACCAAAATCCCTTAACGTGAGTTACGCGTCG TTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGA TCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGC TACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCG AAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGT GTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACAT ACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAG TCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCA GCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAA CGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCC ACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGT CGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATC TTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTG TGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGC CTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT >T2 AAAAGGCCATCCGTCAGGATGGCCTTCT >T1 ATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTAT >M13_Forward GTAAAACGACGGCCAG >attL1 CAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACACAT TGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCT >pME_EGFPCAAX_insert TTTGTACAAAAAAGCAGGCTGGACCATGGTGAGCAAGGGCGAGGAGCTGT TCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGC CACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAA GCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGC CCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTAC CCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGG CTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGA CCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAG CTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCT GGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGA AGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGC AGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGG CCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGA GCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTG ACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGGGAGGAGG AAGATCTAAGCTGAACCCTCCTGATGAGAGTGGCCCCGGCTGCATGAGCT GCAAGTGTGTGCTCTCCTGAGACCCAGCTTTCTTGTACAAAGT >EGFP_w/o_stop ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGT CGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGG GCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACC ACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTA CGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACT TCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTC TTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGG CGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGG ACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAAC GTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAA GATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACC AGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCAC TACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGA TCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCA TGGACGAGCTGTACAAG >CAAX_box_from_human_Harvey_Ras AAGCTGAACCCTCCTGATGAGAGTGGCCCCGGCTGCATGAGCTGCAAGTG TGTGCTCTCCTGA >attL2 ACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCA ATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTG >T7 TACGACTCACTATAGGGGAT >M13_Reverse CAGGAAACAGCTATGAC >kan ATGAGCCATATTCAACGGGAAACGTCGAGGCCGCGATTAAATTCCAACAT GGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAAT CAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTTG TTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGAT GGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGC ATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCC GGAAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAA TATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTG TTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCG CAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGA GCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTT TGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGAT AACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACG AGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCC TCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGT ATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGA GTTTTTCTAA >pUC_ori CCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGT AATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTT TGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGC AGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCA CCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCC TGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTG GACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGG GGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGA GATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGA AAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCAC GAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGT TTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGG CGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGC CTTTTGCTGGCCTTTTGCTCACAT