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Revision as of 02:16, 24 March 2007 by Chi-bin (talk | contribs) (New page: <pre> >pME_Gal4VP16 CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCA...)
>pME_Gal4VP16 CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGA AAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAA GAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTA GTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGG CCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCA GGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCC GACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGT TAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCC AGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGAC CTGTTCGTTGCAACACATTGATGAGCAATGCTTTTTTATAATGCCAACTT TGTACAAAAAAGCAGGCTGGACCATGAAGCTACTGTCTTCTATCGAACAA GCATGCGATATTTGCCGACTTAAAAAGCTCAAGTGCTCCAAAGAAAAACC GAAGTGCGCCAAGTGTCTGAAGAACAACTGGGAGTGTCGCTACTCTCCCA AAACCAAAAGGTCTCCGCTGACTAGGGCACATCTGACAGAAGTGGAATCA AGGCTAGAAAGACTGGAACAGCTATTTCTACTGATTTTTCCTCGAGAAGA CCTTGACATGATTTTGAAAATGGATTCTTTACAGGATATAAAAGCATTGT TAACAGGATTATTTGTACAAGATAATGTGAATAAAGATGCCGTCACAGAT AGATTGGCTTCAGTGGAGACTGATATGCCTCTAACATTGAGACAGCATAG AATAAGTGCGACATCATCATCGGAAGAGAGTAGTAACAAAGGTCAAAGAC AGTTGACTGTATCTTCGAGGTCGACCCCGGGAATTCAGATCTCTCGAGCC GCCCCCCCGACCGATGTCAGCCTGGGGGACGAGCTCCACTTAGACGGCGA GGACGTGGCGATGGCGCATGCCGACGCGCTAGACGATTTCGATCTGGACA TGTTGGGGGACGGGGATTCCCCGGGTCCGGGATTTACCCCCCACGACTCC GCCCCCTACGGCGCTCTGGATATGTAGCACCCAGCTTTCTTGTACAAAGT TGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCA CTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTAT AGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGT GTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAATAATATCATCAT GAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATG AGCCATATTCAACGGGAAACGTCGAGGCCGCGATTAAATTCCAACATGGA TGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAG GTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTTGTTT CTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGT CAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATT TTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGA AAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATAT TGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTT GTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAA TCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCG TAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGC CATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAAC CTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGT CGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCG GTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATT GATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTT TTTCTAATCAGAATTGGTTAATTGGTTGTAACACTGGCAGAGCATTACGC TGACTTGACGGGACGGCGCAAGCTCATGACCAAAATCCCTTAACGTGAGT TACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATC TTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAA AACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACT CTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGT TCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAC CGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGT GGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGA TAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCT TGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGA GAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAG CGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACG CCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGT CGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAG CAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACA TGTT >T2 AAAAGGCCATCCGTCAGGATGGCCTTCT >T1 ATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTAT >attL1 CAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACACAT TGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCT >pME_Gal4VP16v2_insert TTTGTACAAAAAAGCAGGCTGGACCATGAAGCTACTGTCTTCTATCGAAC AAGCATGCGATATTTGCCGACTTAAAAAGCTCAAGTGCTCCAAAGAAAAA CCGAAGTGCGCCAAGTGTCTGAAGAACAACTGGGAGTGTCGCTACTCTCC CAAAACCAAAAGGTCTCCGCTGACTAGGGCACATCTGACAGAAGTGGAAT CAAGGCTAGAAAGACTGGAACAGCTATTTCTACTGATTTTTCCTCGAGAA GACCTTGACATGATTTTGAAAATGGATTCTTTACAGGATATAAAAGCATT GTTAACAGGATTATTTGTACAAGATAATGTGAATAAAGATGCCGTCACAG ATAGATTGGCTTCAGTGGAGACTGATATGCCTCTAACATTGAGACAGCAT AGAATAAGTGCGACATCATCATCGGAAGAGAGTAGTAACAAAGGTCAAAG ACAGTTGACTGTATCTTCGAGGTCGACCCCGGGAATTCAGATCTCTCGAG CCGCCCCCCCGACCGATGTCAGCCTGGGGGACGAGCTCCACTTAGACGGC GAGGACGTGGCGATGGCGCATGCCGACGCGCTAGACGATTTCGATCTGGA CATGTTGGGGGACGGGGATTCCCCGGGTCCGGGATTTACCCCCCACGACT CCGCCCCCTACGGCGCTCTGGATATGTAGCACCCAGCTTTCTTGTACAAA GT >GAL4,_aa1-147 ATGAAGCTACTGTCTTCTATCGAACAAGCATGCGATATTTGCCGACTTAA AAAGCTCAAGTGCTCCAAAGAAAAACCGAAGTGCGCCAAGTGTCTGAAGA ACAACTGGGAGTGTCGCTACTCTCCCAAAACCAAAAGGTCTCCGCTGACT AGGGCACATCTGACAGAAGTGGAATCAAGGCTAGAAAGACTGGAACAGCT ATTTCTACTGATTTTTCCTCGAGAAGACCTTGACATGATTTTGAAAATGG ATTCTTTACAGGATATAAAAGCATTGTTAACAGGATTATTTGTACAAGAT AATGTGAATAAAGATGCCGTCACAGATAGATTGGCTTCAGTGGAGACTGA TATGCCTCTAACATTGAGACAGCATAGAATAAGTGCGACATCATCATCGG AAGAGAGTAGTAACAAAGGTCAAAGACAGTTGACTGTATC >VP16_activating_domain GCCCCCCCGACCGATGTCAGCCTGGGGGACGAGCTCCACTTAGACGGCGA GGACGTGGCGATGGCGCATGCCGACGCGCTAGACGATTTCGATCTGGACA TGTTGGGGGACGGGGATTCCCCGGGTCCGGGATTTACCCCCCACGACTCC GCCCCCTACGGCGCTCTGGATATG >attL2 ACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCA ATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTG >T7 TACGACTCACTATAGGGGAT >M13_Reverse CAGGAAACAGCTATGAC >kan ATGAGCCATATTCAACGGGAAACGTCGAGGCCGCGATTAAATTCCAACAT GGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAAT CAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTTG TTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGAT GGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGC ATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCC GGAAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAA TATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTG TTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCG CAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGA GCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTT TGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGAT AACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACG AGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCC TCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGT ATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGA GTTTTTCTAA >pUC_ori CCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGT AATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTT TGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGC AGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCA CCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCC TGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTG GACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGG GGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGA GATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGA AAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCAC GAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGT TTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGG CGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGC CTTTTGCTGGCCTTTTGCTCACAT