From Tol2Kit
Revision as of 03:32, 24 March 2007 by Chi-bin (talk | contribs) (New page: <pre> >pCS2FA-transposase TCGACCATAGCCAATTCAATATGGCGTATATGGACTCATGCCAATTCAAT ATGGTGGATCTGGACCTGTGCCAATTCAATATGGCGTATATGGACTCGTG CCAATTCAATATGGTGGATCTGGACCCCAGCCAATTCAATATGGCGGACT TGGCACCAT...)
>pCS2FA-transposase TCGACCATAGCCAATTCAATATGGCGTATATGGACTCATGCCAATTCAAT ATGGTGGATCTGGACCTGTGCCAATTCAATATGGCGTATATGGACTCGTG CCAATTCAATATGGTGGATCTGGACCCCAGCCAATTCAATATGGCGGACT TGGCACCATGCCAATTCAATATGGCGGACTTGGCACTGTGCCAACTGGGG AGGGGTCTACTTGGCACGGTGCCAAGTTTGAGGAGGGGTCTTGGCCCTGT GCCAAGTCCGCCATATTGAATTGGCATGGTGCCAATAATGGCGGCCATAT TGGCTATATGCCAGGATCAATATATAGGCAATATCCAATATGGCCCTATG CCAATATGGCTATTGGCCAGGTTCAATACTATGTATTGGCCCTATGCCAT ATAGTATTCCATATATGGGTTTTCCTATTGACGTAGATAGCCCCTCCCAA TGGGCGGTCCCATATACCATATATGGGGCTTCCTAATACCGCCCATAGCC ACTCCCCCATTGACGTCAATGGTCTCTATATATGGTCTTTCCTATTGACG TCATATGGGCGGTCCTATTGACGTATATGGCGCCTCCCCCATTGACGTCA ATTACGGTAAATGGCCCGCCTGGCTCAATGCCCATTGACGTCAATAGGAC CACCCACCATTGACGTCAATGGGATGGCTCATTGCCCATTCATATCCGTT CTCACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCACTTGGCAGTA CATCAATATCTATTAATAGTAACTTGGCAAGTACATTACTATTGGAAGTA CGCCAGGGTACATTGGCAGTACTCCCATTGACGTCAATGGCGGTAAATGG CCCGCGATGGCTGCCAAGTACATCCCCATTGACGTCAATGGGGAGGGGCA ATGACGCAAATGGGCGTTCCATTGACGTAAATGGGCGGTAGGCGTGCCTA ATGGGAGGTCTATATAAGCAATGCTCGTTTAGGGAACCGCCATTCTGCCT GGGGACGTCGGAGCAAGCTTGATTTAGGTGACACTATAGAATACAAGCTA CTTGTTCTTTTTGCAGGATCCGGCCGGCCACCATGGAGGAAGTATGTGAT TCATCAGCAGCTGCGAGCAGCACAGTCCAAAATCAGCCACAGGATCAAGA GCACCCGTGGCCGTATCTTCGCGAATTCTTTTCTTTAAGTGGTGTAAATA AAGATTCATTCAAGATGAAATGTGTCCTCTGTCTCCCGCTTAATAAAGAA ATATCGGCCTTCAAAAGTTCGCCATCAAACCTAAGGAAGCATATTGAGAG AATGCACCCAAATTACCTCAAAAACTACTCTAAATTGACAGCACAGAAGA GAAAGATCGGGACCTCCACCCATGCTTCCAGCAGTAAGCAACTGAAAGTT GACTCAGTTTTCCCAGTCAAACATGTGTCTCCAGTCACTGTGAACAAAGC TATATTAAGGTACATCATTCAAGGACTTCATCCTTTCAGCACTGTTGATC TGCCATCATTTAAAGAGCTGATTAGTACACTGCAGCCTGGCATTTCTGTC ATTACAAGGCCTACTTTACGCTCCAAGATAGCTGAAGCTGCTCTGATCAT GAAACAGAAAGTGACTGCTGCCATGAGTGAAGTTGAATGGATTGCAACCA CAACGGATTGTTGGACTGCACGTAGAAAGTCATTCATTGGTGTAACTGCT CACTGGATCAACCCTGGAAGTCTTGAAAGACATTCCGCTGCACTTGCCTG CAAAAGATTAATGGGCTCTCATACTTTTGAGGTACTGGCCAGTGCCATGA ATGATATCCACTCAGAGTATGAAATACGTGACAAGGTTGTTTGCACAACC ACAGACAGTGGTTCCAACTTTATGAAGGCTTTCAGAGTTTTTGGTGTGGA AAACAATGATATCGAGACTGAGGCAAGAAGGTGTGAAAGTGATGACACTG ATTCTGAAGGCTGTGGTGAGGGAAGTGATGGTGTGGAATTCCAAGATGCC TCACGAGTCCTGGACCAAGACGATGGCTTCGAATTCCAGCTACCAAAACA TCAAAAGTGTGCCTGTCACTTACTTAACCTAGTCTCAAGCGTTGATGCCC AAAAAGCTCTCTCAAATGAACACTACAAGAAACTCTACAGATCTGTCTTT GGCAAATGCCAAGCTTTATGGAATAAAAGCAGCCGATCGGCTCTAGCAGC TGAAGCTGTTGAATCAGAAAGCCGGCTTCAGCTTTTAAGGCCAAACCAAA CGCGGTGGAATTCAACTTTTATGGCTGTTGACAGAATTCTTCAAATTTGC AAAGAAGCAGGAGAAGGCGCACTTCGGAATATATGCACCTCTCTTGAGGT TCCAATGTTTAATCCAGCAGAAATGCTGTTCTTGACAGAGTGGGCCAACA CAATGCGTCCAGTTGCAAAAGTACTCGACATCTTGCAAGCGGAAACGAAT ACACAGCTGGGGTGGCTGCTGCCTAGTGTCCATCAGTTAAGCTTGAAACT TCAGCGACTCCACCATTCTCTCAGGTACTGTGACCCACTTGTGGATGCCC TACAACAAGGAATCCAAACACGATTCAAGCATATGTTTGAAGATCCTGAG ATCATAGCAGCTGCCATCCTTCTCCCTAAATTTCGGACCTCTTGGACAAA TGATGAAACCATCATAAAACGAGGCATGGACTACATCAGAGTGCATCTGG AGCCTTTGGACCACAAGAAGGAATTGGCCAACAGTTCATCTGATGATGAA GATTTTTTCGCTTCTTTGAAACCGACAACACATGAAGCCAGCAAAGAGTT GGATGGATATCTGGCCTGTGTTTCAGACACCAGGGAGTCTCTGCTCACGT TTCCTGCTATTTGCAGCCTCTCTATCAAGACTAATACACCTCTTCCCGCA TCGGCTGCCTGTGAGAGGCTTTTCAGCACTGCAGGATTGCTTTTCAGCCC CAAAAGAGCTAGGCTTGACACTAACAATTTTGAGAATCAGCTTCTACTGA AGTTAAATCTGAGGTTTTACAACTTTGAGTAGGGCGCGCCTCTAGAACTA TAGTGAGTCGTATTACGTAGATCCAGACATGATAAGATACATTGATGAGT TTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAA ATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACA AGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGG TGTGGGAGGTTTTTTAATTCGCGGCCGCGGCGCCAATGCATTGGGCCCGG TACCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGCGCTTGGCGTAA TCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCC ACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAAT GAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAG TCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGG GAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACT CGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAG GCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACAT GTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGC TGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGA CGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGC GTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGC TTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCT CATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAA GCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTAT CCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCA CTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGG TGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGA CAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGA GTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTT TTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAG ATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCA CGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGAT CCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGT AAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCA GCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTA GATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGA TACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAG CCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTC CATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAG TTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCA CGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAG GCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCG GTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATG GTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATG CTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTA TGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCG CCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGG GCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAAC CCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTT TCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAG GGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATT GAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGT ATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGT GCCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTT TTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCC CTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTT TGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCG AAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAAT CAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAA GGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAG AAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTG TAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCG CTACAGGGCGCGTCCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGG GCGATCGGTGCGGGCCTCTTCGCTATTACGCCAG >sCMV_IE94_enhancer/promoter TCGACCATAGCCAATTCAATATGGCGTATATGGACTCATGCCAATTCAAT ATGGTGGATCTGGACCTGTGCCAATTCAATATGGCGTATATGGACTCGTG CCAATTCAATATGGTGGATCTGGACCCCAGCCAATTCAATATGGCGGACT TGGCACCATGCCAATTCAATATGGCGGACTTGGCACTGTGCCAACTGGGG AGGGGTCTACTTGGCACGGTGCCAAGTTTGAGGAGGGGTCTTGGCCCTGT GCCAAGTCCGCCATATTGAATTGGCATGGTGCCAATAATGGCGGCCATAT TGGCTATATGCCAGGATCAATATATAGGCAATATCCAATATGGCCCTATG CCAATATGGCTATTGGCCAGGTTCAATACTATGTATTGGCCCTATGCCAT ATAGTATTCCATATATGGGTTTTCCTATTGACGTAGATAGCCCCTCCCAA TGGGCGGTCCCATATACCATATATGGGGCTTCCTAATACCGCCCATAGCC ACTCCCCCATTGACGTCAATGGTCTCTATATATGGTCTTTCCTATTGACG TCATATGGGCGGTCCTATTGACGTATATGGCGCCTCCCCCATTGACGTCA ATTACGGTAAATGGCCCGCCTGGCTCAATGCCCATTGACGTCAATAGGAC CACCCACCATTGACGTCAATGGGATGGCTCATTGCCCATTCATATCCGTT CTCACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCACTTGGCAGTA CATCAATATCTATTAATAGTAACTTGGCAAGTACATTACTATTGGAAGGA CGCCAGGGTACATTGGCAGTACTCCCATTGACGTCAATGGCGGTAAATGG CCCGCGATGGCTGCCAAGTACATCCCCATTGACGTCAATGGGGAGGGGCA ATGACGCAAATGGGCGTTCCATTGACGTAAATGGGCGGTAGGCGTGCCTA ATGGGAGGTCTATATAAGCAATGCTCGTTTAGGGAAC >SP6_promoter ATTTAGGTGACACTATA >beta-globin_5'_leader CTTGTTCTTTTTGCA >transposase ATGGAGGAAGTATGTGATTCATCAGCAGCTGCGAGCAGCACAGTCCAAAA TCAGCCACAGGATCAAGAGCACCCGTGGCCGTATCTTCGCGAATTCTTTT CTTTAAGTGGTGTAAATAAAGATTCATTCAAGATGAAATGTGTCCTCTGT CTCCCGCTTAATAAAGAAATATCGGCCTTCAAAAGTTCGCCATCAAACCT AAGGAAGCATATTGAGAGAATGCACCCAAATTACCTCAAAAACTACTCTA AATTGACAGCACAGAAGAGAAAGATCGGGACCTCCACCCATGCTTCCAGC AGTAAGCAACTGAAAGTTGACTCAGTTTTCCCAGTCAAACATGTGTCTCC AGTCACTGTGAACAAAGCTATATTAAGGTACATCATTCAAGGACTTCATC CTTTCAGCACTGTTGATCTGCCATCATTTAAAGAGCTGATTAGTACACTG CAGCCTGGCATTTCTGTCATTACAAGGCCTACTTTACGCTCCAAGATAGC TGAAGCTGCTCTGATCATGAAACAGAAAGTGACTGCTGCCATGAGTGAAG TTGAATGGATTGCAACCACAACGGATTGTTGGACTGCACGTAGAAAGTCA TTCATTGGTGTAACTGCTCACTGGATCAACCCTGGAAGTCTTGAAAGACA TTCCGCTGCACTTGCCTGCAAAAGATTAATGGGCTCTCATACTTTTGAGG TACTGGCCAGTGCCATGAATGATATCCACTCAGAGTATGAAATACGTGAC AAGGTTGTTTGCACAACCACAGACAGTGGTTCCAACTTTATGAAGGCTTT CAGAGTTTTTGGTGTGGAAAACAATGATATCGAGACTGAGGCAAGAAGGT GTGAAAGTGATGACACTGATTCTGAAGGCTGTGGTGAGGGAAGTGATGGT GTGGAATTCCAAGATGCCTCACGAGTCCTGGACCAAGACGATGGCTTCGA ATTCCAGCTACCAAAACATCAAAAGTGTGCCTGTCACTTACTTAACCTAG TCTCAAGCGTTGATGCCCAAAAAGCTCTCTCAAATGAACACTACAAGAAA CTCTACAGATCTGTCTTTGGCAAATGCCAAGCTTTATGGAATAAAAGCAG CCGATCGGCTCTAGCAGCTGAAGCTGTTGAATCAGAAAGCCGGCTTCAGC TTTTAAGGCCAAACCAAACGCGGTGGAATTCAACTTTTATGGCTGTTGAC AGAATTCTTCAAATTTGCAAAGAAGCAGGAGAAGGCGCACTTCGGAATAT ATGCACCTCTCTTGAGGTTCCAATGTTTAATCCAGCAGAAATGCTGTTCT TGACAGAGTGGGCCAACACAATGCGTCCAGTTGCAAAAGTACTCGACATC TTGCAAGCGGAAACGAATACACAGCTGGGGTGGCTGCTGCCTAGTGTCCA TCAGTTAAGCTTGAAACTTCAGCGACTCCACCATTCTCTCAGGTACTGTG ACCCACTTGTGGATGCCCTACAACAAGGAATCCAAACACGATTCAAGCAT ATGTTTGAAGATCCTGAGATCATAGCAGCTGCCATCCTTCTCCCTAAATT TCGGACCTCTTGGACAAATGATGAAACCATCATAAAACGAGGCATGGACT ACATCAGAGTGCATCTGGAGCCTTTGGACCACAAGAAGGAATTGGCCAAC AGTTCATCTGATGATGAAGATTTTTTCGCTTCTTTGAAACCGACAACACA TGAAGCCAGCAAAGAGTTGGATGGATATCTGGCCTGTGTTTCAGACACCA GGGAGTCTCTGCTCACGTTTCCTGCTATTTGCAGCCTCTCTATCAAGACT AATACACCTCTTCCCGCATCGGCTGCCTGTGAGAGGCTTTTCAGCACTGC AGGATTGCTTTTCAGCCCCAAAAGAGCTAGGCTTGACACTAACAATTTTG AGAATCAGCTTCTACTGAAGTTAAATCTGAGGTTTTACAACTTTGAGTAG >SV40_polyA_in_pCS2+ GATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAG AATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTT TATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGC ATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAA >T3_primer AATTAACCCTCACTAAAGGG >M13R GGAAACAGCTATGACCATGA >pUC_ori TGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCT GGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGAC GCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCG TTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCT TACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC ATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAG CTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATC CGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCAC TGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGT GCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGAC AGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAG TTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTT TTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGA TCCTTTGATCTTTTCTAC >amp ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATT TTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATG CTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGAT GAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACG CCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGT AAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCA ACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCT GAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAA TGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACC ACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTG GAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAC TATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTA AGCATTGG >f1_(+)_ori GCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACT TGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCG CCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTA GGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTA GGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCC CTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACT GGAACAA