From Tol2Kit
Revision as of 23:11, 2 November 2007 by Chi-bin (talk | contribs) (New page: === Construction details === pME-EGFP no stop was made by PCR amplification of the EGFP insert from a pCS2-based plasmid, using primers that add att sites, followed by a BP reaction. Sequ...)
Construction details
pME-EGFP no stop was made by PCR amplification of the EGFP insert from a pCS2-based plasmid, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct.
This clone can be used to generate N-terminal EGFP fusions with a gene of interest placed in a 3' clone.
Crudely annotated sequence
FASTA file with the full-length sequence as well as sequences of individual components:
pME-EGFP no stop sequence
Crude map
Screenshot from Sequencher showing locations of components:
