From Tol2Kit
Construction details
pME-mCherry no stop was made by PCR amplification of mCherry, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct.
This clone can be used to generate N-terminal mCherry fusions with a gene of interest placed in a 3' clone.
Crudely annotated sequence
FASTA file with the full-length sequence as well as sequences of individual components:
pME-mCherry no stop sequence
Crude map
Screenshot from Sequencher showing locations of components:
