From Tol2Kit
Revision as of 02:41, 19 December 2007 by Chi-bin (talk | contribs) (New page: <pre> LOCUS 299.p5E-bactin2 7950 bp ds-DNA circular 10-DEC-2007 REFERENCE 1 AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, Parant ...)
LOCUS 299.p5E-bactin2 7950 bp ds-DNA circular 10-DEC-2007
REFERENCE 1
AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS,
Parant JM, Yost HJ, Kanki JP, Chien CB.
TITLE The Tol2kit: a multisite gateway-based construction kit for Tol2
transposon transgenesis constructs.
JOURNAL Developmental Dynamics 236(11):3088-99 (2007).
PUBMED 17937395
COMMENT 299.p5E-bactin2, Tol2kit v1.0
COMMENT This clone contains 3.8 kb of upstream genomic sequence from the bactin2 gene, plus the first (5' UTR) exon, first intron (1.3 kb), and 8 bp of the second exon (including the end of the 5' UTR and going right up to just before the ATG). Construct generated by Kristen Kwan (Chien lab, University of Utah), using PCR from a ~5 kb bactin2 genomic fragment made by Ken Poss (Keating lab, University of Utah) from a longer clone from Shin-ichi Higashijima. Sequence and annotation 11/6/06 by Chi-Bin Chien, based on genomic sequence and predicted exons from the Sanger Centre.
COMMENT ApEinfo:methylated:1
FEATURES Location/Qualifiers
misc_recomb 668..688
/label=attB4
/ApEinfo_fwdcolor=#5d40ff
/ApEinfo_revcolor=#5d40ff
misc_recomb 593..688
/label=attL4
/ApEinfo_fwdcolor=#5d40ff
/ApEinfo_revcolor=#5d40ff
misc_recomb 5996..6152
/label=attR1
/ApEinfo_fwdcolor=#3fd0ff
/ApEinfo_revcolor=#3fd0ff
misc_feature 6000..6013
/label=att1_shared
/ApEinfo_fwdcolor=#32a6cc
/ApEinfo_revcolor=#32a6cc
misc_feature 668..688
/label=att4_shared
/ApEinfo_fwdcolor=#4a33cc
/ApEinfo_revcolor=#4a33cc
promoter 691..5994
/label=bactin2 -5.3kb to -5bp
/ApEinfo_fwdcolor=#ffffbf
/ApEinfo_revcolor=#ffffbf
5'UTR 4541..4626
/label=bactin2 exon1 (5' UTR)
/ApEinfo_fwdcolor=#ffff3f
/ApEinfo_revcolor=#ffff3f
intron 4627..5987
/label=bactin2 intron1
/ApEinfo_fwdcolor=#ffff7f
/ApEinfo_revcolor=#ffff7f
rep_origin 7274..7947
/label=pUC ori
/ApEinfo_fwdcolor=#999999
/ApEinfo_revcolor=#999999
CDS 6344..7153
/label=kanR
/ApEinfo_fwdcolor=#ff7f3f
/ApEinfo_revcolor=#ff7f3f
primer_bind complement(6211..6231)
/label=M13R
/ApEinfo_fwdcolor=#777777
/ApEinfo_revcolor=#777777
primer_bind 537..552
/label=M13F
/ApEinfo_fwdcolor=#777777
/ApEinfo_revcolor=#777777
promoter complement(6188..6207)
/label=T7
/ApEinfo_fwdcolor=#777777
/ApEinfo_revcolor=#777777
terminator 268..295
/label=T2
/ApEinfo_fwdcolor=#bbbbbb
/ApEinfo_revcolor=#bbbbbb
terminator 427..470
/label=T1
/ApEinfo_fwdcolor=#bbbbbb
/ApEinfo_revcolor=#bbbbbb
ORIGIN
1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
541 AACGACGGCC AGTCTTAAGC TCGGGCCCGC GTTAACGCTA CCATGGAGCT CCAAATAATG
601 ATTTTATTTT GACTGATAGT GACCTGTTCG TTGCAACAAA TTGATAAGCA ATGCTTTTTT
661 ATAATGCCAA CTTTGTATAG AAAAGTTGAA AATTCCAGTT TGAAGAAACT TTTCAAGACT
721 CACCTTTTCA CTGTTGGTCT TAATTTAGGT TGATTATTTT TGTCAATTTA ATAATTACAT
781 TGTATTGTTC ATATTTTATT GTCTTATTGT GGTGTTTTTA CAGCTTTTTT ATTGTTCTGC
841 TTTGTTACTG TTTGCCTTGT ATGCATTGTA GCGCTTTGGG TCTGAGAAAA GCGCACTATA
901 AATAAACTGT ATTATTATTA TTAGGGATGG ACATCACGGT GGCGCGATGG GTGGCACAAT
961 TACCTCACAG CAAGTAGGTT GCTGTTTAGA GCCCCGGCTG GATCAGTTGG CAGTTCTGTG
1021 TGGAGTTTGC ATGTTCTCCC TGTTCAAGTA GGTTTCCTCC CGGTGCTCTG GTTTCCCCTC
1081 ACAAGTCCAA AGACATGATG TATAGGTGAA TGGGGTAAGC TAAATTGACC ATAGTGAGTG
1141 TGTGTGTGTG TGTGAATGAG AGTGTATAGG TGTTTCCCAG TGTTAGGTTG CAACTGGAAG
1201 GACATCTGCT GTGTAAAACA TATGCAGGAT AAGTTGGCGG TTCATTCCCC TATGGTGACC
1261 CCTGGTTAAT TAAAGGACTA AGTTGAAAAT GAATGAATGA ATGAATGATT AGAGATAAAC
1321 CGATACCACT TTTTTACAAA CCGATATGAG TACAAGTATA ATAGTTTCTG TTACTCGACA
1381 ATACTGAGTA CCGATTCTGA TACTTCTTGG ATTTGGTTTC AATGTAATTA ATTTGAAAGA
1441 AGGTTTTATT TCTTGCCTGT CGCAAGGATG AACACAAAAA TCTTTACCAG AAGGATATTT
1501 TAAGCTAAGA ATATACGCAC GTTGTTGACA ACCCTGTATC GATTACCGAT TAAACGTGGA
1561 CATTTAAAGG ATAAGTTATG CTTGCGCCAT CACAACTTTA CACATTACAT GATCAAAGAT
1621 CATATTGAAT ATTCGGTTTA TTCGTCAGAA GCCTATAGAT TTGTTAAAAG TGTATTTTAT
1681 AAACGGATGG TTTATAAAGA CTAAGCTATT AATATATCAG ACATTCATTA ATACATCAAG
1741 ACATCGATGT CAGGTAGGAC AACAAATCAC ATCCATGTAA TGAAATCTTG TTGAGTGGTA
1801 TTGGTGCGTG GTTTCAGCAT TTCATGTGCA ATACGAGTAA GAATATTTTA ACCAAGTATC
1861 GACCCAAAAC CTGATACTGG TATCGGTGCA TCCCTTATTA TTATTATTAT CAAAAACACA
1921 CTTGTTTTAT TTATGTGGGT GCCAAACAAC AAACTATGAA ATGCAGCATG ACACGCCCAA
1981 AAAGTGTTCC TTTGAGAACA ACTTCATAAA TATGTTTCTT ATCATTATGC ATGAATGGTG
2041 TACTGGGAGA GCTTTGGTAT CAGAATTGAG ATAAGAGACT TTGGCATTCT AAAGGACAAT
2101 GACATTATCA CACTGACAGT CAACAACACT TTATTGCTCT GGAAATCTTA TGTCATGGGG
2161 CAAAGCTGAG GTTCATTAGG ACAGTACCTT ATTTAGAGAT GAATGGGAAG GCTGTAGTGG
2221 GCTGCTATCA GGGAAGGGTG GTGGTCCTCG GGGGAACTAT GGACTCACAA AAGCACATCT
2281 GCTGTAACAA GATCCCCTGT GCAATCTACA AAAGTTACAA AGAGAAGCAT GGTTGGTTAA
2341 AAAATAGAAT TGACTGGATA TGCTGAATGC ATTGTTTGTT GTGTGTTACA TCACATTAAC
2401 CACAGATTGG TGCTAAATAT TTGCTTATCT TATAAAAACC TGTGTGTTTA TAAAGGGGGG
2461 TGTGGATGGA TGCTTGGCAT TCTTTGAAAA GATGTTGGGG GATGGAAGGA GGAGACCTTA
2521 TTGATGAGTA TCCAGTTTGC AGATAACAAG TTTTTAGACA TTCATACTAT GATGCTGTTT
2581 ATGTCTGTTT TTTCTCATTT TTTCTATACA CAAACAGCTA TAAAACATTC ACTTTTATAT
2641 CTGATGCTGT ATATTAAATT TCGTTGTATT TATTCTGTTT GCTCTGTTGG GTCATAGCTA
2701 GGGCCAGACA GAATCTGCTG ACATTTTTTG CTTTTTCTGC AGAGAATTTT GTAAACAATC
2761 TGTGATTTAT GCGAGATTTT TTCGGGAGTA TCGTAACTAA AAACTTACTA TATGAAATAA
2821 AAAAAAATAA TACCTTTTTA ACTTTTAATA AATATATACA ATGCAAGTCC ACCTAGATCC
2881 AATTATTTGG TCGACAAAGC AAGTCTCTCA CATAATATAT CTTCTAAAAG ACAGAAAACA
2941 TTACTTTCAG ACCATATTGT AATCAAATGA ACACTTTCAT ATTAGTCAAT AATATTACGG
3001 AAATGTATTT AAAAACTGAA TAAACATATA TTTACACCTT TGTCTTTATT TCTAGAGTGC
3061 TTTTACAATG TAGATTGTGT CAAAGCAGCT TAACTTAGAG GATTAGTATA GTAAATTGAA
3121 ACTGTGTCAG TCCATTTTTT AAGAGTTTAA GTTCAGTTCA GTTTAGTTCA GTGTGAGTTA
3181 ATTTTTACTG CTGAGAGTCC AAACACTAAA GAGCAAATCC AAATACACAA GTCAATAAAT
3241 AAACTCAATG ATGGGCTAAA GATCTGGGGA AATCTGCAGA TTTCTGTGCG CACAGATTCT
3301 GTGTGGGCCT AGTCATTACC TATACTCAAC GTTTACTCTG AGAAACTTCC ATATTTTAGA
3361 GGTAAAATGC TCAGAATTGT AAAAAGTCTT GTCAAAATAC TTGGAATTTG CCAAAAACAA
3421 AATAAAAGTC ACATCACCTC ATTGTCCTTG GTATCAGCAA GCACTTTTAA CACAATCAGC
3481 TGTTTTTGTT TATAACTCCC ACTGAAGGCC ATGTGCATTA CTCCCAAGTT ACACAGTAAA
3541 AAGTCACATG TAGTTTATCA TGATCACTGC TGCTCAAGCA CAGCTTTATC TGTTATTCAG
3601 TGTGGCAATA TCTGAAATGT GTGCAAAATA TTATTTATTG TAGCTGATCA CCAACACTTT
3661 CAGTGTTTTT TTTTTCTACT TTTAATGAGA ATTATGTTCT TAGGATTTAT CCTTATAAAC
3721 CTCTTTAAAA AGAAATGTTA TGTCTGCATA GACACGACAT GAAATTATGA ACAGAAAATT
3781 TAACTATTTA AGATCAAGAC TTGACATATA ACATTTCTAA TGAACATTAA ACCTGAGGTA
3841 GGCCCACTTG CAAAAATGTA ACTGCTATCA CTCACTTTAT ACATCATTGT GTAACTTGTA
3901 CACACAAAAA TAGTGAAGCA ATGTGGCATG GAATGCAGGT CCCATTCATT CAAAGTTATT
3961 AAAGCACTTT CTTAAAGTGC ATTACTACTG AGCTAAGACC AGCTAGTTGC TCATACAACA
4021 TACTACAAGT AGTGTCATCC TAATTTGGAT AAAAAAAATG TCCATTTTAT ACATGGAAAA
4081 GTACTCATTA GATCCCCCAT TGTTTGTATT ACGGTATTTC GTGAACACAA GAGGTAAATG
4141 ACCTACAGAG CTGCTGTTGT GTTAGATTGA AAACACAACA CAGGATCATG GAGGCATCCA
4201 TCATTATGAC GCATTCTGCC ACTTGTAACT CTGCACAGAC TTTTTGAAAA GTTTAATTGA
4261 GTCTTGGCGT TGTCGCAGAA AAATACGAAA AATTCTGTGT TAAAATTTGC AAATAAAAAT
4321 TCCAGTTTTA TAGGAAATCG CCATGGCCTT GTTCTTTAAC CAGTTAAGCT TCCCCTTCTT
4381 TCACTCTCAA GTTGCAAGAA GCAAGTGTAG CAATGTGCAC GCGACAGCCG GGTGTGTGAC
4441 GCTGGACCAA TCAGAGCACA GAGCTCCGAA AGTTTACCTT TTATGGCTAG AGCCGGGTAT
4501 GTGCCGTCAT ATAAAAGAGC GCGCCCAGCT TTTCAGCCTC ACTTTGAGCT CCTCCACACG
4561 CAGCTAGTGC GGAATATCAT CTGCTTGTAA CCCATTCTCT TAAGTCGACA ACCCCCCAAA
4621 CCCAAGGTGA GTTGGTTTTA CAGTTTTTAA GCGTTTAATT ATAGTTTCTA TCTATTAATG
4681 TTAAGTAAAA TGTGTAAATG GATAAATATG ATTTGATGAA GTCTTTAGAC AGTTCATAAC
4741 GGTCTCTTTG TAGTTATCTA CTAGGTTTGA TTTATAAGTG GTGGTTCTTT TGATAATCTA
4801 TGTTACATTT AATAAATATT GGCTGTATCT TAACTTTTTT GTAGCGTTAG TGGATGTGGC
4861 AGGTGAGAAT GTCGGTAATT TAACGTGACC AGTCGTAGGC ACGACATTGA ACATTGAATA
4921 GGCCGGTGTG AAATGGGTGT TAAGTCTTAA TATAAAGTTG TGTTCTGGCT AATCTCTCTT
4981 TAACAGCCAT AAATGTGATT TTTAACTACA GTTTTGAGGG TGCTGGTGAG CATGTTGCAC
5041 ACTTGATGGA TAGCCGGCAT GGGAAGTTCT TTGTGCAGGC AGTGCTGCAG CAGGGTGTGA
5101 CCTACTTTAG CTAGCCGGCT AACCAGCTCT CATCTGATGT AACGTAAACC CCATCAATCT
5161 AACATCGGCT CATTCGTGCT TTATAAGATC GAAGATCTTG TTATTAGTAA CGGGTATTGG
5221 CATTTAGTTT TAACTTGGTT AATTTTCTTT TTGTTATGTG TTCTATTTAA ATGTAGCCTG
5281 CAGCCCCCTC TGTCTTTAAT GAAGCTGAAG ATTAACGTTA ACGTTAGTGA AGTGTTGTAT
5341 CCGCTATGAA AGATTTGAAT TTTAGAGGTG TGGTCCACTT TAATAACGGC TGCGGAGTTC
5401 TCTGGCTGCA CTTCAGCCGC TGTGACTGTC GAAGCAGAGC CCATCAGTTT TTCAGGATCT
5461 CGGTGATTTT TGAGGCTAGA CTATGAACTG AACCGACTGA GAGTGACCGT CCAGAAATGC
5521 TTTAGTGTGA AGTCTCCGCT CACGAGGAGT CCGTGGACTG ACTGCAGATC TGTGACGCAG
5581 TAACACCCGG CGGCAGACAC CCGTTGAAAT GCGGTTGTGT AATTGATCGC AGGCGAGGGT
5641 GGAAGAGGAT GTGAAACTTC ATTTGTGTAA AATTTAGGGA GTGGTCCTGG TGTGATGAAT
5701 GTCGAAATCT GTTCCTTTTT ACTGAGCCCT ACGACTCTGG CTGAGTGCCA CACCGCCGGC
5761 AGCCGCAAAG CGGCTCAAAC CATTGCCTTT TATGGTAATA ATGAGAGAAT GCAGAGGGAC
5821 TTCCTTTGTC TGGCATATCT GAGGCGCGCG CTGTCACTAG CGCCCACCAG CGGTCAGACT
5881 GTAGAATGCA GAGCAAAACA GGAAGTTGAC TCCAGATGGT CACATGCTTG CTGAAACGCC
5941 TCTCCCTGGC AGCAGTGCAA TTCTAACAGT TTCCCTTTCT TTTACAGTTC AGCCTCAAGT
6001 TTGTACAAAA AAGTTGAACG AGAAACGTAA AATGATATAA ATATCAATAT ATTAAATTAG
6061 ATTTTGCATA AAAAACAGAC TACATAATAC TGTAAAACAC AACATATGCA GTCACTATGA
6121 ACCAACTACT TAGATGGTAT TAGTGACCTG TAGAATTCGA GCTCTAGAGC TGCAGGGCGG
6181 CCGCGATATC CCCTATAGTG AGTCGTATTA CATGGTCATA GCTGTTTCCT GGCAGCTCTG
6241 GCCCGTGTCT CAAAATCTCT GATGTTACAT TGCACAAGAT AAAAATATAT CATCATGAAC
6301 AATAAAACTG TCTGCTTACA TAAACAGTAA TACAAGGGGT GTTATGAGCC ATATTCAACG
6361 GGAAACGTCG AGGCCGCGAT TAAATTCCAA CATGGATGCT GATTTATATG GGTATAAATG
6421 GGCTCGCGAT AATGTCGGGC AATCAGGTGC GACAATCTAT CGCTTGTATG GGAAGCCCGA
6481 TGCGCCAGAG TTGTTTCTGA AACATGGCAA AGGTAGCGTT GCCAATGATG TTACAGATGA
6541 GATGGTCAGA CTAAACTGGC TGACGGAATT TATGCCTCTT CCGACCATCA AGCATTTTAT
6601 CCGTACTCCT GATGATGCAT GGTTACTCAC CACTGCGATC CCCGGAAAAA CAGCATTCCA
6661 GGTATTAGAA GAATATCCTG ATTCAGGTGA AAATATTGTT GATGCGCTGG CAGTGTTCCT
6721 GCGCCGGTTG CATTCGATTC CTGTTTGTAA TTGTCCTTTT AACAGCGATC GCGTATTTCG
6781 TCTCGCTCAG GCGCAATCAC GAATGAATAA CGGTTTGGTT GATGCGAGTG ATTTTGATGA
6841 CGAGCGTAAT GGCTGGCCTG TTGAACAAGT CTGGAAAGAA ATGCATAAAC TTTTGCCATT
6901 CTCACCGGAT TCAGTCGTCA CTCATGGTGA TTTCTCACTT GATAACCTTA TTTTTGACGA
6961 GGGGAAATTA ATAGGTTGTA TTGATGTTGG ACGAGTCGGA ATCGCAGACC GATACCAGGA
7021 TCTTGCCATC CTATGGAACT GCCTCGGTGA GTTTTCTCCT TCATTACAGA AACGGCTTTT
7081 TCAAAAATAT GGTATTGATA ATCCTGATAT GAATAAATTG CAGTTTCATT TGATGCTCGA
7141 TGAGTTTTTC TAATCAGAAT TGGTTAATTG GTTGTAACAC TGGCAGAGCA TTACGCTGAC
7201 TTGACGGGAC GGCGCAAGCT CATGACCAAA ATCCCTTAAC GTGAGTTACG CGTCGTTCCA
7261 CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG
7321 CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA
7381 TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA
7441 TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC
7501 TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG
7561 TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC
7621 GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT
7681 ACAGCGTGAG CATTGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC
7741 GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG
7801 GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG
7861 CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT
7921 GGCCTTTTGC TGGCCTTTTG CTCACATGTT
//