From Tol2Kit
Revision as of 03:59, 19 December 2007 by Chi-bin (talk | contribs) (New page: <pre> LOCUS 382.p5E-CMV/SP6 3704 bp ds-DNA circular 18-DEC-2007 REFERENCE 1 AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, Parant ...)
LOCUS 382.p5E-CMV/SP6 3704 bp ds-DNA circular 18-DEC-2007
REFERENCE 1
AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS,
Parant JM, Yost HJ, Kanki JP, Chien CB.
TITLE The Tol2kit: a multisite gateway-based construction kit for Tol2
transposon transgenesis constructs.
JOURNAL Developmental Dynamics 236(11):3088-99 (2007).
PUBMED 17937395
COMMENT 382.p5E-CMV/SP6, Tol2kit v1.0
COMMENT ApEinfo:methylated:1
FEATURES Location/Qualifiers
misc_recomb 668..688
/label=attB4
/ApEinfo_fwdcolor=#5d40ff
/ApEinfo_revcolor=#5d40ff
misc_recomb 593..688
/label=attL4
/ApEinfo_fwdcolor=#5d40ff
/ApEinfo_revcolor=#5d40ff
misc_recomb 1750..1906
/label=attR1
/ApEinfo_fwdcolor=#3fd0ff
/ApEinfo_revcolor=#3fd0ff
misc_feature 1754..1767
/label=att1_shared
/ApEinfo_fwdcolor=#32a6cc
/ApEinfo_revcolor=#32a6cc
misc_feature 668..688
/label=att4_shared
/ApEinfo_fwdcolor=#4a33cc
/ApEinfo_revcolor=#4a33cc
promoter 719..1696
/label=sCMV IE94
/ApEinfo_fwdcolor=#ffffbf
/ApEinfo_revcolor=#ffffbf
rep_origin 3028..3701
/label=pUC ori
/ApEinfo_fwdcolor=#999999
/ApEinfo_revcolor=#999999
CDS 2098..2907
/label=kanR
/ApEinfo_fwdcolor=#ff7f3f
/ApEinfo_revcolor=#ff7f3f
primer_bind complement(1965..1985)
/label=M13R
/ApEinfo_fwdcolor=#777777
/ApEinfo_revcolor=#777777
primer_bind 537..552
/label=M13F
/ApEinfo_fwdcolor=#777777
/ApEinfo_revcolor=#777777
promoter complement(1942..1961)
/label=T7
/ApEinfo_fwdcolor=#777777
/ApEinfo_revcolor=#777777
promoter 1731..1747
/label=SP6
/ApEinfo_fwdcolor=#ffff00
/ApEinfo_revcolor=#ffff00
terminator 268..295
/label=T2
/ApEinfo_fwdcolor=#bbbbbb
/ApEinfo_revcolor=#bbbbbb
terminator 427..470
/label=T1
/ApEinfo_fwdcolor=#bbbbbb
/ApEinfo_revcolor=#bbbbbb
ORIGIN
1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
541 AACGACGGCC AGTCTTAAGC TCGGGCCCGC GTTAACGCTA CCATGGAGCT CCAAATAATG
601 ATTTTATTTT GACTGATAGT GACCTGTTCG TTGCAACAAA TTGATAAGCA ATGCTTTTTT
661 ATAATGCCAA CTTTGTATAG AAAAGTTGAA GGCCTCTTCG CTATTACGCC AGTCGACCGC
721 CAATTCAATA TGGCGTATAT GGACTCATGC CAATTCAATA TGGTGGATCT GGACCTGTGC
781 CAATTCAATA TGGCGTATAT GGACTCGTGC CAATTCAATA TGGTGGATCT GGACCCCAGC
841 CAATTCAATA TGGCGGACTT GGCACCATGC CAATTCAATA TGGCGGACCT GGCACTGTGC
901 CAACTGGGGA GGGGTCTACT TGGCACGGTG CCAAGTTTGA GGAGGGGTCT TGGCCCTGTG
961 CCAAGTCCGC CATATTGAAT TGGCATGGTG CCAATAATGG CGGCCATATT GGCTATATGC
1021 CAGGATCAAT ATATAGGCAA TATCCAATAT GGCCCTATGC CAATATGGCT ATTGGCCAGG
1081 TTCAATACTA TGTATTGGCC CTATGCCATA TAGTATTCCA TATATGGGTT TTCCTATTGA
1141 CGTAGATAGC CCCTCCCAAT GGGCGGTCCC ATATACCATA TATGGGGCTT CCTAATACCG
1201 CCCATAGCCA CTCCCCCATT GACGTCAATG GTCTCTATAT ATGGTCTTTC CTATTGACGT
1261 CATATGGGCG GTCCTATTGA CGTATATGGC GCCTCCCCCA TTGACGTCAA TTACGGTAAA
1321 TGGCCCGCCT GGCTCAATGC CCATTGACGT CAATAGGACC ACCCACCATT GACGTCAATG
1381 GGATGGCTCA TTGCCCATTC ATATCCGTTC TCACGCCCCC TATTGACGTC AATGACGGTA
1441 AATGGCCCAC TTGGCAGTAC ATCAATATCT ATTAATAGTA ACTTGGCAAG TACATTACTA
1501 TTGGAAGTAC GCCAGGGTAC ATTGGCAGTA CTCCCATTGA CGTCAATGGC GGTAAATGGC
1561 CCGCGATGGC TGCCAAGTAC ATCCCCATTG ACGTCAATGG GGAGGGGCAA TGACGCAAAT
1621 GGGCGTTCCA TTGACGTAAA TGGGCGGTAG GCGTGCCTAA TGGGAGGTCT ATATAAGCAA
1681 TGCTCGTTTA GGGAACCGCC ATTCTGCCTG GGGACGTCGG AGCAAGCTTG ATTTAGGTGA
1741 CACTATAGAC AAGTTTGTAC AAAAAAGTTG AACGAGAAAC GTAAAATGAT ATAAATATCA
1801 ATATATTAAA TTAGATTTTG CATAAAAAAC AGACTACATA ATACTGTAAA ACACAACATA
1861 TGCAGTCACT ATGAACCAAC TACTTAGATG GTATTAGTGA CCTGTAGAAT TCGAGCTCTA
1921 GAGCTGCAGG GCGGCCGCGA TATCCCCTAT AGTGAGTCGT ATTACATGGT CATAGCTGTT
1981 TCCTGGCAGC TCTGGCCCGT GTCTCAAAAT CTCTGATGTT ACATTGCACA AGATAAAAAT
2041 ATATCATCAT GAACAATAAA ACTGTCTGCT TACATAAACA GTAATACAAG GGGTGTTATG
2101 AGCCATATTC AACGGGAAAC GTCGAGGCCG CGATTAAATT CCAACATGGA TGCTGATTTA
2161 TATGGGTATA AATGGGCTCG CGATAATGTC GGGCAATCAG GTGCGACAAT CTATCGCTTG
2221 TATGGGAAGC CCGATGCGCC AGAGTTGTTT CTGAAACATG GCAAAGGTAG CGTTGCCAAT
2281 GATGTTACAG ATGAGATGGT CAGACTAAAC TGGCTGACGG AATTTATGCC TCTTCCGACC
2341 ATCAAGCATT TTATCCGTAC TCCTGATGAT GCATGGTTAC TCACCACTGC GATCCCCGGA
2401 AAAACAGCAT TCCAGGTATT AGAAGAATAT CCTGATTCAG GTGAAAATAT TGTTGATGCG
2461 CTGGCAGTGT TCCTGCGCCG GTTGCATTCG ATTCCTGTTT GTAATTGTCC TTTTAACAGC
2521 GATCGCGTAT TTCGTCTCGC TCAGGCGCAA TCACGAATGA ATAACGGTTT GGTTGATGCG
2581 AGTGATTTTG ATGACGAGCG TAATGGCTGG CCTGTTGAAC AAGTCTGGAA AGAAATGCAT
2641 AAACTTTTGC CATTCTCACC GGATTCAGTC GTCACTCATG GTGATTTCTC ACTTGATAAC
2701 CTTATTTTTG ACGAGGGGAA ATTAATAGGT TGTATTGATG TTGGACGAGT CGGAATCGCA
2761 GACCGATACC AGGATCTTGC CATCCTATGG AACTGCCTCG GTGAGTTTTC TCCTTCATTA
2821 CAGAAACGGC TTTTTCAAAA ATATGGTATT GATAATCCTG ATATGAATAA ATTGCAGTTT
2881 CATTTGATGC TCGATGAGTT TTTCTAATCA GAATTGGTTA ATTGGTTGTA ACACTGGCAG
2941 AGCATTACGC TGACTTGACG GGACGGCGCA AGCTCATGAC CAAAATCCCT TAACGTGAGT
3001 TACGCGTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT
3061 CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG
3121 GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA
3181 GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC
3241 TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT
3301 GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG
3361 CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC
3421 GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG
3481 GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA
3541 GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT
3601 CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC
3661 TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTT
//