Basic Gateway principles

(under construction. 1/31/07)

Principles of Gateway recombination

Here's a diagram of a Gateway BP reaction, showing an example of how a "middle" clone (pME) is constructed using PCR with gene-specific primers to add att sites, then recombination with a donor vector.

Here's a diagram of a multisite Gateway LR reaction, combining three entry clones (p5E, pME, p3E) with the destination vector pDestTol2pA.