From Tol2Kit
LOCUS 396.pCS2-transposase 6034 bp ds-DNA circular 18-DEC-2007
REFERENCE 1
AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS,
Parant JM, Yost HJ, Kanki JP, Chien CB.
TITLE The Tol2kit: a multisite gateway-based construction kit for Tol2
transposon transgenesis constructs.
JOURNAL Developmental Dynamics 236(11):3088-99 (2007).
PUBMED 17937395
COMMENT 396.pCS2-transposase, Tol2kit v1.0
COMMENT ApEinfo:methylated:1
FEATURES Location/Qualifiers
polyA_signal 3070..3267
/label=SV40 late polyA (from pCS2+)
/ApEinfo_fwdcolor=#ffff7f
/ApEinfo_revcolor=#ffff7f
promoter 10..987
/label=sCMV IE94
/ApEinfo_fwdcolor=#ffffbf
/ApEinfo_revcolor=#ffffbf
rep_origin complement(3699..4372)
/label=pUC ori
/ApEinfo_fwdcolor=#999999
/ApEinfo_revcolor=#999999
rep_origin complement(3740..4422)
/label=colE1 ori
/ApEinfo_fwdcolor=#999999
/ApEinfo_revcolor=#999999
rep_origin complement(5640..5946)
/label=f1(+) ori
/ApEinfo_fwdcolor=#999999
/ApEinfo_revcolor=#999999
CDS complement(4520..5377)
/label=ampR
/ApEinfo_fwdcolor=#ff7f00
/ApEinfo_revcolor=#ff7f00
primer_bind complement(3351..3372)
/label=M13R
/ApEinfo_fwdcolor=#777777
/ApEinfo_revcolor=#777777
promoter complement(3316..3335)
/label=T3
/ApEinfo_fwdcolor=#777777
/ApEinfo_revcolor=#777777
promoter 1022..1038
/label=SP6
/ApEinfo_fwdcolor=#ffff00
/ApEinfo_revcolor=#ffff00
misc_feature 1083..3032
/label=Tol2 transposase cds
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=green
ORIGIN
1 TCGACCATAG CCAATTCAAT ATGGCGTATA TGGACTCATG CCAATTCAAT ATGGTGGATC
61 TGGACCTGTG CCAATTCAAT ATGGCGTATA TGGACTCGTG CCAATTCAAT ATGGTGGATC
121 TGGACCCCAG CCAATTCAAT ATGGCGGACT TGGCACCATG CCAATTCAAT ATGGCGGACT
181 TGGCACTGTG CCAACTGGGG AGGGGTCTAC TTGGCACGGT GCCAAGTTTG AGGAGGGGTC
241 TTGGCCCTGT GCCAAGTCCG CCATATTGAA TTGGCATGGT GCCAATAATG GCGGCCATAT
301 TGGCTATATG CCAGGATCAA TATATAGGCA ATATCCAATA TGGCCCTATG CCAATATGGC
361 TATTGGCCAG GTTCAATACT ATGTATTGGC CCTATGCCAT ATAGTATTCC ATATATGGGT
421 TTTCCTATTG ACGTAGATAG CCCCTCCCAA TGGGCGGTCC CATATACCAT ATATGGGGCT
481 TCCTAATACC GCCCATAGCC ACTCCCCCAT TGACGTCAAT GGTCTCTATA TATGGTCTTT
541 CCTATTGACG TCATATGGGC GGTCCTATTG ACGTATATGG CGCCTCCCCC ATTGACGTCA
601 ATTACGGTAA ATGGCCCGCC TGGCTCAATG CCCATTGACG TCAATAGGAC CACCCACCAT
661 TGACGTCAAT GGGATGGCTC ATTGCCCATT CATATCCGTT CTCACGCCCC CTATTGACGT
721 CAATGACGGT AAATGGCCCA CTTGGCAGTA CATCAATATC TATTAATAGT AACTTGGCAA
781 GTACATTACT ATTGGAAGTA CGCCAGGGTA CATTGGCAGT ACTCCCATTG ACGTCAATGG
841 CGGTAAATGG CCCGCGATGG CTGCCAAGTA CATCCCCATT GACGTCAATG GGGAGGGGCA
901 ATGACGCAAA TGGGCGTTCC ATTGACGTAA ATGGGCGGTA GGCGTGCCTA ATGGGAGGTC
961 TATATAAGCA ATGCTCGTTT AGGGAACCGC CATTCTGCCT GGGGACGTCG GAGCAAGCTT
1021 GATTTAGGTG ACACTATAGA ATACAAGCTA CTTGTTCTTT TTGCAGGATC CGGCCGGCCA
1081 CCATGGAGGA AGTATGTGAT TCATCAGCAG CTGCGAGCAG CACAGTCCAA AATCAGCCAC
1141 AGGATCAAGA GCACCCGTGG CCGTATCTTC GCGAATTCTT TTCTTTAAGT GGTGTAAATA
1201 AAGATTCATT CAAGATGAAA TGTGTCCTCT GTCTCCCGCT TAATAAAGAA ATATCGGCCT
1261 TCAAAAGTTC GCCATCAAAC CTAAGGAAGC ATATTGAGAG AATGCACCCA AATTACCTCA
1321 AAAACTACTC TAAATTGACA GCACAGAAGA GAAAGATCGG GACCTCCACC CATGCTTCCA
1381 GCAGTAAGCA ACTGAAAGTT GACTCAGTTT TCCCAGTCAA ACATGTGTCT CCAGTCACTG
1441 TGAACAAAGC TATATTAAGG TACATCATTC AAGGACTTCA TCCTTTCAGC ACTGTTGATC
1501 TGCCATCATT TAAAGAGCTG ATTAGTACAC TGCAGCCTGG CATTTCTGTC ATTACAAGGC
1561 CTACTTTACG CTCCAAGATA GCTGAAGCTG CTCTGATCAT GAAACAGAAA GTGACTGCTG
1621 CCATGAGTGA AGTTGAATGG ATTGCAACCA CAACGGATTG TTGGACTGCA CGTAGAAAGT
1681 CATTCATTGG TGTAACTGCT CACTGGATCA ACCCTGGAAG TCTTGAAAGA CATTCCGCTG
1741 CACTTGCCTG CAAAAGATTA ATGGGCTCTC ATACTTTTGA GGTACTGGCC AGTGCCATGA
1801 ATGATATCCA CTCAGAGTAT GAAATACGTG ACAAGGTTGT TTGCACAACC ACAGACAGTG
1861 GTTCCAACTT TATGAAGGCT TTCAGAGTTT TTGGTGTGGA AAACAATGAT ATCGAGACTG
1921 AGGCAAGAAG GTGTGAAAGT GATGACACTG ATTCTGAAGG CTGTGGTGAG GGAAGTGATG
1981 GTGTGGAATT CCAAGATGCC TCACGAGTCC TGGACCAAGA CGATGGCTTC GAATTCCAGC
2041 TACCAAAACA TCAAAAGTGT GCCTGTCACT TACTTAACCT AGTCTCAAGC GTTGATGCCC
2101 AAAAAGCTCT CTCAAATGAA CACTACAAGA AACTCTACAG ATCTGTCTTT GGCAAATGCC
2161 AAGCTTTATG GAATAAAAGC AGCCGATCGG CTCTAGCAGC TGAAGCTGTT GAATCAGAAA
2221 GCCGGCTTCA GCTTTTAAGG CCAAACCAAA CGCGGTGGAA TTCAACTTTT ATGGCTGTTG
2281 ACAGAATTCT TCAAATTTGC AAAGAAGCAG GAGAAGGCGC ACTTCGGAAT ATATGCACCT
2341 CTCTTGAGGT TCCAATGTTT AATCCAGCAG AAATGCTGTT CTTGACAGAG TGGGCCAACA
2401 CAATGCGTCC AGTTGCAAAA GTACTCGACA TCTTGCAAGC GGAAACGAAT ACACAGCTGG
2461 GGTGGCTGCT GCCTAGTGTC CATCAGTTAA GCTTGAAACT TCAGCGACTC CACCATTCTC
2521 TCAGGTACTG TGACCCACTT GTGGATGCCC TACAACAAGG AATCCAAACA CGATTCAAGC
2581 ATATGTTTGA AGATCCTGAG ATCATAGCAG CTGCCATCCT TCTCCCTAAA TTTCGGACCT
2641 CTTGGACAAA TGATGAAACC ATCATAAAAC GAGGCATGGA CTACATCAGA GTGCATCTGG
2701 AGCCTTTGGA CCACAAGAAG GAATTGGCCA ACAGTTCATC TGATGATGAA GATTTTTTCG
2761 CTTCTTTGAA ACCGACAACA CATGAAGCCA GCAAAGAGTT GGATGGATAT CTGGCCTGTG
2821 TTTCAGACAC CAGGGAGTCT CTGCTCACGT TTCCTGCTAT TTGCAGCCTC TCTATCAAGA
2881 CTAATACACC TCTTCCCGCA TCGGCTGCCT GTGAGAGGCT TTTCAGCACT GCAGGATTGC
2941 TTTTCAGCCC CAAAAGAGCT AGGCTTGACA CTAACAATTT TGAGAATCAG CTTCTACTGA
3001 AGTTAAATCT GAGGTTTTAC AACTTTGAGT AGGGCGCGCC TCTAGAACTA TAGTGAGTCG
3061 TATTACGTAG ATCCAGACAT GATAAGATAC ATTGATGAGT TTGGACAAAC CACAACTAGA
3121 ATGCAGTGAA AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT ATTTGTAACC
3181 ATTATAAGCT GCAATAAACA AGTTAACAAC AACAATTGCA TTCATTTTAT GTTTCAGGTT
3241 CAGGGGGAGG TGTGGGAGGT TTTTTAATTC GCGGCCGCGG CGCCAATGCA TTGGGCCCGG
3301 TACCCAGCTT TTGTTCCCTT TAGTGAGGGT TAATTGCGCG CTTGGCGTAA TCATGGTCAT
3361 AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA
3421 GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC
3481 GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC
3541 AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT
3601 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC
3661 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA
3721 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG
3781 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA
3841 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC
3901 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC
3961 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC
4021 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG
4081 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT
4141 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA
4201 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT
4261 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA
4321 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG
4381 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT
4441 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT
4501 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC
4561 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG
4621 GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG
4681 ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT
4741 TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG
4801 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT
4861 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA
4921 TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG
4981 CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT
5041 CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA
5101 TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA
5161 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT
5221 TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT
5281 CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA
5341 AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT
5401 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA
5461 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTAAA TTGTAAGCGT
5521 TAATATTTTG TTAAAATTCG CGTTAAATTT TTGTTAAATC AGCTCATTTT TTAACCAATA
5581 GGCCGAAATC GGCAAAATCC CTTATAAATC AAAAGAATAG ACCGAGATAG GGTTGAGTGT
5641 TGTTCCAGTT TGGAACAAGA GTCCACTATT AAAGAACGTG GACTCCAACG TCAAAGGGCG
5701 AAAAACCGTC TATCAGGGCG ATGGCCCACT ACGTGAACCA TCACCCTAAT CAAGTTTTTT
5761 GGGGTCGAGG TGCCGTAAAG CACTAAATCG GAACCCTAAA GGGAGCCCCC GATTTAGAGC
5821 TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG AAGAAAGCGA AAGGAGCGGG
5881 CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA ACCACCACAC CCGCCGCGCT
5941 TAATGCGCCG CTACAGGGCG CGTCCCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG
6001 GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAG
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