From Tol2Kit
Revision as of 19:42, 7 February 2007 by Chi-bin (talk | contribs) (New page: '''Construction details'''<br> p3E-IRES-nlsEGFPpA was made by fusion PCR of the EMCV IRES sequence, and nlsEGFP-SV40 late polyA, followed by a BP reaction. Sequencing confirms that all bas...)
Construction details
p3E-IRES-nlsEGFPpA was made by fusion PCR of the EMCV IRES sequence, and nlsEGFP-SV40 late polyA, followed by a BP reaction. Sequencing confirms that all bases of the coding sequence are correct. There is a single base change in the IRES sequence, but we have confirmed functionally that this cassette produces fluorescent, nuclear-localized protein.
Crudely annotated sequence
FASTA file with the full-length sequence as well as sequences of individual components):
p3E-IRES-nlsEGFPpA sequence
Crude map
Screenshot from Sequencher showing locations of components:
