Protocols

Note: The Invitrogen Multi-Site Gateway Manual contains all of the basic information necessary to understand and perform Gateway recombination reactions. The protocols here reflect the methods that the Chien Lab has adopted after much trial and error.

BP Reactions

• Donor Vectors

• PCR Amplification of DNA

• Purification of PCR products

• BP Recombination Reactions

• Transformation, Plasmid Prep, and Diagnostic Digests

LR reactions

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