From Tol2Kit
LOCUS 395.pDestTol2CG2 7796 bp ds-DNA circular 10-DEC-2007
REFERENCE 1
AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS,
Parant JM, Yost HJ, Kanki JP, Chien CB.
TITLE The Tol2kit: a multisite gateway-based construction kit for Tol2
transposon transgenesis constructs.
JOURNAL Developmental Dynamics 236(11):3088-99 (2007).
PUBMED 17937395
COMMENT 395.pDestTol2CG2, Tol2kit v1.0
COMMENT ApEinfo:methylated:1
FEATURES Location/Qualifiers
misc_recomb 2784..2908
/label=attR3
/ApEinfo_fwdcolor=#5370ff
/ApEinfo_revcolor=#5370ff
misc_recomb 1205..1329
/label=attR4
/ApEinfo_fwdcolor=#5d40ff
/ApEinfo_revcolor=#5d40ff
misc_feature 2888..2902
/label=att3_shared
/ApEinfo_fwdcolor=#4259cc
/ApEinfo_revcolor=#4259cc
misc_feature 1205..1225
/label=att4_shared
/ApEinfo_fwdcolor=#4a33cc
/ApEinfo_revcolor=#4a33cc
polyA_signal 2948..3145
/label=SV40 late polyA (from pCS2+)
/ApEinfo_fwdcolor=#ffff7f
/ApEinfo_revcolor=#ffff7f
polyA_signal complement(3204..3336)
/label=SV40 late polyA (from pCS2+)(1)
/ApEinfo_label=SV40 late polyA (from pCS2+)
/ApEinfo_fwdcolor=#ffff7f
/ApEinfo_revcolor=#ffff7f
promoter complement(4104..5002)
/label=cmlc2 promoter
/ApEinfo_fwdcolor=#ffffbf
/ApEinfo_revcolor=#ffffbf
CDS complement(3387..4103)
/label=EGFP
/ApEinfo_fwdcolor=#00ff00
/ApEinfo_revcolor=#00ff00
misc_structure complement(3899..3912)
/label=EGFP-like chromophore
/ApEinfo_fwdcolor=#00cc00
/ApEinfo_revcolor=#00cc00
rep_origin complement(5994..6667)
/label=pUC ori
/ApEinfo_fwdcolor=#999999
/ApEinfo_revcolor=#999999
CDS complement(6815..7672)
/label=ampR
/ApEinfo_fwdcolor=#ff7f00
/ApEinfo_revcolor=#ff7f00
CDS complement(2016..2675)
/label=cmR
/ApEinfo_fwdcolor=#ffbf00
/ApEinfo_revcolor=#ffbf00
misc_feature complement(1369..1674)
/label=ccdB
/ApEinfo_fwdcolor=#ffbf3f
/ApEinfo_revcolor=#ffbf3f
primer_bind 1169..1190
/label=M13R
/ApEinfo_fwdcolor=#777777
/ApEinfo_revcolor=#777777
primer_bind complement(5646..5667)
/label=M13R(1)
/ApEinfo_label=M13R
/ApEinfo_fwdcolor=#777777
/ApEinfo_revcolor=#777777
primer_bind complement(2917..2932)
/label=M13F
/ApEinfo_fwdcolor=#777777
/ApEinfo_revcolor=#777777
promoter complement(5611..5630)
/label=T3
/ApEinfo_fwdcolor=#777777
/ApEinfo_revcolor=#777777
LTR complement(622..638)
/label=Tol2 3' TIR
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=#ff0000
LTR complement(5507..5525)
/label=Tol2 5' TIR
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=#ff0000
misc_structure complement(5009..5343)
/label=Tol2 exon1 bp1-335 of 461
/ApEinfo_fwdcolor=#aa0000
/ApEinfo_revcolor=#aa0000
misc_structure complement(811..1157)
/label=Tol2 exon4 136-482
/ApEinfo_fwdcolor=#aa0000
/ApEinfo_revcolor=#aa0000
misc_structure complement(5526..5562)
/label=medaka tyrosinase ex5 frag
/ApEinfo_fwdcolor=#006600
/ApEinfo_revcolor=#006600
gene 337..622
/label=medaka tyrosinase genomic (from AB010101)
/ApEinfo_fwdcolor=#006600
/ApEinfo_revcolor=#006600
ORIGIN
1 CCACCTAAAT TGTAAGCGTT AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA
61 GCTCATTTTT TAACCAATAG GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGA
121 CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA AAGAACGTGG
181 ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA TGGCCCACTA CGTGAACCAT
241 CACCCTAATC AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC ACTAAATCGG AACCCTAAAG
301 GGAGCCCCCG ATTTAGAGCT TGACGGGGAA AGCCGGGCTG GCTAAGAACT CATCAGCCTC
361 CCCGGTCCAT CTACCCACGT ACCAATGCAC CAATTGGCCA CAATGACGGC TACTACATGG
421 TGCCATTCCT TCCTCTTTAT AGGAATGGAG ACTACCTCCT GTCCAACAAG GCTCTTGGAT
481 ACGAGTACGC CTACCTGTTG GACCCAGGTC ATTGCACAAC ACCAGAAATG CCCTCTGATC
541 TGCAAAAGAC GTGAATATCT GTTCAGACAC CCATATCCAC TCTGTTCCAC ACAGGTCAGA
601 GGTTTGTCCA GGAGTTCTTG ACAGAGGTGT AAAAAGTACT CAAAAATTTT ACTCAAGTGA
661 AAGTACAAGT ACTTAGGGAA AATTTTACTC AATTAAAAGT AAAAGTATCT GGCTAGAATC
721 TTACTTGAGT AAAAGTAAAA AAGTACTCCA TTAAAATTGT ACTTGAGTAT TAAGGAAGTA
781 AAAGTAAAAG CAAGAAAGAA AACTAGAGAT TCTTGTTTAA GCTTTTAATC TCAAAAAACA
841 TTAAATGAAA TGCATACAAG GTTTTATCCT GCTTTAGAAC TGTTTGTATT TAATTATCAA
901 ACTATAAGAC AGACAATCTA ATGCCAGTAC ACGCTACTCA AAGTTGTAAA ACCTCAGATT
961 TAACTTCAGT AGAAGCTGAT TCTCAAAATT GTTAGTGTCA AGCCTAGCTC TTTTGGGGCT
1021 GAAAAGCAAT CCTGCAGTGC TGAAAAGCCT CTCACAGGCA GCCGATGCGG GAAGAGGTGT
1081 ATTAGTCTTG ATAGAGAGGC TGCAAATAGC AGGAAACGTG AGCAGAGACT CCCTGGTGTC
1141 TGAAACACAG GCCAGATGGG CCCTCGAGCA GGAAACAGCT ATGACCATGA TTACGCCAAG
1201 CTATCAACTT TGTATAGAAA AGTTGAACGA GAAACGTAAA ATGATATAAA TATCAATATA
1261 TTAAATTAGA TTTTGCATAA AAAACAGACT ACATAATACT GTAAAACACA ACATATCCAG
1321 TCACTATGGT CGACCTGCAG ACTGGCTGTG TATAAGGGAG CCTGACATTT ATATTCCCCA
1381 GAACATCAGG TTAATGGCGT TTTTGATGTC ATTTTCGCGG TGGCTGAGAT CAGCCACTTC
1441 TTCCCCGATA ACGGAGACCG GCACACTGGC CATATCGGTG GTCATCATGC GCCAGCTTTC
1501 ATCCCCGATA TGCACCACCG GGTAAAGTTC ACGGGGGACT TTATCTGACA GCAGACGTGC
1561 ACTGGCCAGG GGGATCACCA TCCGTCGCCC GGGCGTGTCA ATAATATCAC TCTGTACATC
1621 CACAAACAGA CGATAACGGC TCTCTCTTTT ATAGGTGTAA ACCTTAAACT GCATTTCACC
1681 AGCCCCTGTT CTCGTCGGCA AAAGAGCCGT TCATTTCAAT AAACCGGGCG ACCTCAGCCA
1741 TCCCTTCCTG ATTTTCCGCT TTCCAGCGTT CGGCACGCAG ACGACGGGCT TCATTCTGCA
1801 TGGTTGTGCT TACCGAACCG GAGATATTGA CATCATATAT GCCTTGAGCA ACTGATAGCT
1861 GTCGCTGTCA ACTGTCACTG TAATACGCTG CTTCATAGCA TACCTCTTTT TGACATACTT
1921 CGGGTATACA TATCAGTATA TATTCTTATA CCGCAAAAAT CAGCGCGCAA ATACGCATAC
1981 TGTTATCTGG CTTTTAGTAA GCCGGATCCT CTAGATTACG CCCCGCCCTG CCACTCATCG
2041 CAGTACTGTT GTAATTCATT AAGCATTCTG CCGACATGGA AGCCATCACA AACGGCATGA
2101 TGAACCTGAA TCGCCAGCGG CATCAGCACC TTGTCGCCTT GCGTATAATA TTTGCCCATG
2161 GTGAAAACGG GGGCGAAGAA GTTGTCCATA TTGGCCACGT TTAAATCAAA ACTGGTGAAA
2221 CTCACCCAGG GATTGGCTGA GACGAAAAAC ATATTCTCAA TAAACCCTTT AGGGAAATAG
2281 GCCAGGTTTT CACCGTAACA CGCCACATCT TGCGAATATA TGTGTAGAAA CTGCCGGAAA
2341 TCGTCGTGGT ATTCACTCCA GAGCGATGAA AACGTTTCAG TTTGCTCATG GAAAACGGTG
2401 TAACAAGGGT GAACACTATC CCATATCACC AGCTCACCGT CTTTCATTGC CATACGGAAT
2461 TCCGGATGAG CATTCATCAG GCGGGCAAGA ATGTGAATAA AGGCCGGATA AAACTTGTGC
2521 TTATTTTTCT TTACGGTCTT TAAAAAGGCC GTAATATCCA GCTGAACGGT CTGGTTATAG
2581 GTACATTGAG CAACTGACTG AAATGCCTCA AAATGTTCTT TACGATGCCA TTGGGATATA
2641 TCAACGGTGG TATATCCAGT GATTTTTTTC TCCATTTTAG CTTCCTTAGC TCCTGAAAAT
2701 CTCGACGGAT CCTAACTCAA AATCCACACA TTATACGAGC CGGAAGCATA AAGTGTAAAG
2761 CCTGGGGGTG CCTAATGCGG CCGCCATAGT GACTGGATAT GTTGTGTTTT ACAGTATTAT
2821 GTAGTCTGTT TTTTATGCAA AATCTAATTT AATATATTGA TATTTATATC ATTTTACGTT
2881 TCTCGTTCAA CTTTATTATA CATAGTTGAT AATTCACTGG CCGTCGTTTT ACGGTACCAT
2941 CGATGATGAT CCAGACATGA TAAGATACAT TGATGAGTTT GGACAAACCA CAACTAGAAT
3001 GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT
3061 TATAAGCTGC AATAAACAAG TTAACAACAA CAATTGCATT CATTTTATGT TTCAGGTTCA
3121 GGGGGAGGTG TGGGAGGTTT TTTAAAGCAA GTAAAACCTC TACAAATGTG GTATGGCTGA
3181 TTATGATCCT CTAGATCAGA TCTCTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC
3241 AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG
3301 TCCAAACTCA TCAATGTATC TTATCATGTC TGGATCTACG TAATACGACT CACTATAGTT
3361 CTAGAGGCTC GAGAGGGGCC GCTTTACTTG TACAGCTCGT CCATGCCGAG AGTGATCCCG
3421 GCGGCGGTCA CGAACTCCAG CAGGACCATG TGATCGCGCT TCTCGTTGGG GTCTTTGCTC
3481 AGGGCGGACT GGGTGCTCAG GTAGTGGTTG TCGGGCAGCA GCACGGGGCC GTCGCCGATG
3541 GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC TGCACGCTGC CGTCCTCGAT GTTGTGGCGG
3601 ATCTTGAAGT TCACCTTGAT GCCGTTCTTC TGCTTGTCGG CCATGATATA GACGTTGTGG
3661 CTGTTGTAGT TGTACTCCAG CTTGTGCCCC AGGATGTTGC CGTCCTCCTT GAAGTCGATG
3721 CCCTTCAGCT CGATGCGGTT CACCAGGGTG TCGCCCTCGA ACTTCACCTC GGCGCGGGTC
3781 TTGTAGTTGC CGTCGTCCTT GAAGAAGATG GTGCGCTCCT GGACGTAGCC TTCGGGCATG
3841 GCGGACTTGA AGAAGTCGTG CTGCTTCATG TGGTCGGGGT AGCGGCTGAA GCACTGCACG
3901 CCGTAGGTCA GGGTGGTCAC GAGGGTGGGC CAGGGCACGG GCAGCTTGCC GGTGGTGCAG
3961 ATGAACTTCA GGGTCAGCTT GCCGTAGGTG GCATCGCCCT CGCCCTCGCC GGACACGCTG
4021 AACTTGTGGC CGTTTACGTC GCCGTCCAGC TCGACCAGGA TGGGCACCAC CCCGGTGAAC
4081 AGCTCCTCGC CCTTGCTCAC CATGGTCACT GTCTGCTTTG CTGTTGGTCT GGGCTCCTGG
4141 GTCACTGGCT TACTAATGGA GTCTTTATGT ATGAGGACTC TTATCAATTG TTCTTCTATA
4201 AAGGTCTGCA GTGTTTCTGT TCGTCCCCTA CATGGACACC CAGAGCCTCC TAAATACAGG
4261 AGCCCTGATA ACTGCACAAG TGCTCAGATT CCAGCAGGGT GGAAAATGAG ATAAAGTGTG
4321 CAGATGGGGA GGGGGACGTG AATGAGAGAT TTGAGGGATG AAAAGGATGG ATGAACGCAT
4381 TGAAAATAGC CCCTTTCACA AAATAATACC AGTAAATTGT CATATAATTA ATGACCAGAC
4441 CTTTACCTGT AAATGATGTG ATGTTCATAT ACAGAAGAAT GCCAACAATT TACAGATGAT
4501 TTTACAAATT CTTAATTTTC CTTTTCTAAA TGGATTTGTT CACACATTAT CTCTTCAAAG
4561 CAATTGAATT TTCTGGAAAA GATTGGCTGT GTGTGTAAAA GGTGCTACTG AGGAATTTAA
4621 TGTGACATGG AATGAAGCAA ACAGCAGCCT AAGACAGGAT GGCAGGAAAA ATGTCACATG
4681 TTTAAAATAA GAGTGAAACC AACGACCTGG ACTTATAGAG ACTGTGTGTC CTTAGGCAAA
4741 GCTCTTGTAC AAACAGTGCT TGTGACTGTG AAATTCGGGG TTTGCCTGGA TTGTGTTAAA
4801 TGTGTGCTTG GATGTATCAT AAAAAAGAAT CGGTTCTCTG TATTTGACAT TTTCTGTAAT
4861 TTATCTAAAG TTACAACTGG CTATGCCTGA TTTTTATCAC TATTGAATCA ATCATTTTAA
4921 AGAATGTTAA GTCTGCTGAT TTGGAGAGCT CATTTACATT TATTTTGAAT GTCTCTTATT
4981 TAACACAACT GATTTAAGCT TTAGATCTGC GAAGATACGG CCACGGGTGC TCTTGATCCT
5041 GTGGCTGATT TTGGACTGTG CTGCTCGCAG CTGCTGATGA ATCACATACT TCCTCCATTT
5101 TCTTCCACTG ATTGACTGTT ATAATTTCCC TAATTTCCAG GTCAAGGTGC TGTGCATTGT
5161 GGTAATAGAT GTGACATGAC GTCACTTCCA AAGGACCAAT GAACATGTCT GACCAATTTC
5221 ATATAATGTG AAAACGATTT TCATAGGCAG AATAAATAAC ATTTAAATTA AACTGGGCAT
5281 CAGCGCAATT CAATTGGTTT GGTAATAGCA AGGGAAAATA GAATGAAGTG ATCTCCAAAA
5341 AATAAGTACT TTTTGACTGT AAATAAAATT GTAAGGAGTA AAAAGTACTT TTTTTTCTAA
5401 AAAAATGTAA TTAAGTAAAA GTAAAAGTAT TGATTTTTAA TTGTACTCAA GTAAAGTAAA
5461 AATCCCCAAA AATAATACTT AAGTACAGTA ATCAAGTAAA ATTACTCAAG TACTTTACAC
5521 CTCTGGTTCT TGACCCCCTA CCTTCAGCAA GCCCAGCAGA TCCACTAGTT CTAGAGCGGC
5581 CGCCACCGCG GTGGAGCTCC AGCTTTTGTT CCCTTTAGTG AGGGTTAATT GCGCGCTTGG
5641 CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA
5701 ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA
5761 CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC
5821 ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT
5881 CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT
5941 CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG
6001 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA
6061 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
6121 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
6181 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
6241 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
6301 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
6361 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
6421 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
6481 GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
6541 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
6601 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT
6661 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT
6721 TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT
6781 AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA
6841 TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA
6901 CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC
6961 GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA
7021 GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG
7081 TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG
7141 TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG
7201 TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG
7261 TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC
7321 TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT
7381 TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA
7441 CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA
7501 AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA
7561 ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC
7621 AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC
7681 TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
7741 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTG
//