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(New page: == att site shared sequences == Here is a set of "shared" sequences--the core stretch that is shared between attB/P/L/R for att1-4. These are useful for stitching together entry and destin...)
 
(att site shared sequences)
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<pre>
 
<pre>
>attB1/P1/L1_shared
+
>att1_shared
TTTGTACAAAAAAGC
+
TTTGTACAAAAAAG
>attB2/P2/L2_shared
+
>att2_shared
CAGCTTTCTTGTACAAAGT
+
CTTTCTTGTACAAAGT
>attB3/L3/R3_shared
+
>att3_shared
 
CAACTTTATTATACA
 
CAACTTTATTATACA
>attB4/L4/R4_shared
+
>att4_shared
 
CAACTTTGTATAGAAAAGTTG
 
CAACTTTGTATAGAAAAGTTG
 
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Revision as of 00:31, 10 December 2007

att site shared sequences

Here is a set of "shared" sequences--the core stretch that is shared between attB/P/L/R for att1-4. These are useful for stitching together entry and destination sequences to calculate the sequence of a final expression clone.

>att1_shared
TTTGTACAAAAAAG
>att2_shared
CTTTCTTGTACAAAGT
>att3_shared
CAACTTTATTATACA
>att4_shared
CAACTTTGTATAGAAAAGTTG

list of att sites

Below are all of the att sequences (attB, P, L, and R) used in the original three-part multisite Gateway system. These sequences come from the Invitrogen documentation, and we have seen a few single-base differences from the actual sequences of our clones (it's unclear whether these are documentation mistakes or mutations that have accumulated over time).

>attB1
GGGGACAAGTTTGTACAAAAAAGCAGGCT
>attB1R
GGGGACTGCTTTTTTGTACAAACTTG
>attB2
GGGGACAGCTTTCTTGTACAAAGTGG
>attB2R
GGGGACCACTTTGTACAAGAAAGCTGGGT
>attB3R
GGGGACAACTTTGTATAATAAAGTTG
>attB4
GGGGACAACTTTGTATAGAAAAGTTG
>attL1
CAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACACAT
TGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCT
>attL2
ACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCA
ATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTG
>attL3
CAACTTTATTATACAAAGTTGGCATTATAAAAAAGCATTGCTTATCAATT
TGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTT
>attL4
AAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATT
GATAAGCAATGCTTTTTTATAATGCCAACTTTGTATAGAAAAGTTG
>attP1
AAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACACATT
GATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCTGAACG
AGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATA
AAAAACAGACTACATAATACTGTAAAACACAACATATCCAGTCACTATGA
ATCAACTACTTAGATGGTATTAGTGACCTGTA
>attP1R
AAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATT
GATAAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGAACG
AGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATA
AAAAACAGACTACATAATACTGTAAAACACAACATATGCAGTCACTATGA
ATCAACTACTTAGATGGTATTAGTGACCTGTA
>attP2
AATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTG
ATAAGCAATGCTTTCTTATAATGCCAACTTTGTACAAGAAAGCTGAACGA
GAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAA
AAAACAGACTACATAATACTGTAAAACACAACATATCCAGTCACTATGAA
TCAACTACTTAGATGGTATTAGTGACCTGTA
>attP2R
TACAGGTCACTAATACCATCTAAGTAGTTGATTCATAGTGACTGCATATG
TTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTA
ATATATTGATATTTATATCATTTTACGTTTCTCGTTCAACTTTCTTGTAC
AAAGTTGGCATTATAAAAAAGCATTGCTTATCAATTTGTTGCAACGAACA
GGTCACTATCAGTCAAAATAAAATCATTATTT
>attP3
TACAGGTCACTAATACCATCTAAGTAGTTGATTCATAGTGACTGCATATG
TTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTA
ATATATTGATATTTATATCATTTTACGTTTCTCGTTCAACTTTATTATAC
AAAGTTGGCATTATAAAAAAGCATTGCTTATCAATTTGTTGCAACGAACA
GGTCACTATCAGTCAAAATAAAATCATTATTT
>attP4
AAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATT
GATAAGCAATGCTTTTTTATAATGCCAACTTTGTATAGAAAAGTTGAACG
AGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATA
AAAAACAGACTACATAATACTGTAAAACACAACATATGCAGTCACTATGA
ATCAACTACTTAGATGGTATTAGTGACCTGTA
>attR1
CAAGTTTGTACAAAAAAGTTGAACGAGAAACGTAAAATGATATAAATATC
AATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAA
AACACAACATATGCAGTCACTATGAATCAACTACTTAGATGGTATTAGTG
ACCTGTA
>attR2
TACAGGTCACTAATACCATCTAAGTAGTTGATTCATAGTGACTGCATATG
TTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTA
ATATATTGATATTTATATCATTTTACGTTTCTCGTTCAACTTTCTTGTAC
AAAGTGG
>attR3
CCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTT
TATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCT
CGTTCAACTTTATTATACATAGTTG
>attR4
CAACTTTGTATAGAAAAGTTGAACGAGAAACGTAAAATGATATAAATATC
AATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAA
AACACAACATATCCAGTCACTATGG