From Tol2Kit
Revision as of 14:34, 24 February 2007 by Chi-bin (talk | contribs) (New page: == att site shared sequences == Here is a set of "shared" sequences--the core stretch that is shared between attB/P/L/R for att1-4. These are useful for stitching together entry and destin...)
Here is a set of "shared" sequences--the core stretch that is shared between attB/P/L/R for att1-4. These are useful for stitching together entry and destination sequences to calculate the sequence of a final expression clone.
>attB1/P1/L1_shared TTTGTACAAAAAAGC >attB2/P2/L2_shared CAGCTTTCTTGTACAAAGT >attB3/L3/R3_shared CAACTTTATTATACA >attB4/L4/R4_shared CAACTTTGTATAGAAAAGTTG
list of att sites
Below are all of the att sequences (attB, P, L, and R) used in the original three-part multisite Gateway system. These sequences come from the Invitrogen documentation, and we have seen a few single-base differences from the actual sequences of our clones (it's unclear whether these are documentation mistakes or mutations that have accumulated over time).
>attB1 GGGGACAAGTTTGTACAAAAAAGCAGGCT >attB1R GGGGACTGCTTTTTTGTACAAACTTG >attB2 GGGGACAGCTTTCTTGTACAAAGTGG >attB2R GGGGACCACTTTGTACAAGAAAGCTGGGT >attB3R GGGGACAACTTTGTATAATAAAGTTG >attB4 GGGGACAACTTTGTATAGAAAAGTTG >attL1 CAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACACAT TGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCT >attL2 ACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCA ATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTG >attL3 CAACTTTATTATACAAAGTTGGCATTATAAAAAAGCATTGCTTATCAATT TGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTT >attL4 AAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATT GATAAGCAATGCTTTTTTATAATGCCAACTTTGTATAGAAAAGTTG >attP1 AAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACACATT GATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCTGAACG AGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATA AAAAACAGACTACATAATACTGTAAAACACAACATATCCAGTCACTATGA ATCAACTACTTAGATGGTATTAGTGACCTGTA >attP1R AAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATT GATAAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGAACG AGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATA AAAAACAGACTACATAATACTGTAAAACACAACATATGCAGTCACTATGA ATCAACTACTTAGATGGTATTAGTGACCTGTA >attP2 AATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTG ATAAGCAATGCTTTCTTATAATGCCAACTTTGTACAAGAAAGCTGAACGA GAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAA AAAACAGACTACATAATACTGTAAAACACAACATATCCAGTCACTATGAA TCAACTACTTAGATGGTATTAGTGACCTGTA >attP2R TACAGGTCACTAATACCATCTAAGTAGTTGATTCATAGTGACTGCATATG TTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTA ATATATTGATATTTATATCATTTTACGTTTCTCGTTCAACTTTCTTGTAC AAAGTTGGCATTATAAAAAAGCATTGCTTATCAATTTGTTGCAACGAACA GGTCACTATCAGTCAAAATAAAATCATTATTT >attP3 TACAGGTCACTAATACCATCTAAGTAGTTGATTCATAGTGACTGCATATG TTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTA ATATATTGATATTTATATCATTTTACGTTTCTCGTTCAACTTTATTATAC AAAGTTGGCATTATAAAAAAGCATTGCTTATCAATTTGTTGCAACGAACA GGTCACTATCAGTCAAAATAAAATCATTATTT >attP4 AAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATT GATAAGCAATGCTTTTTTATAATGCCAACTTTGTATAGAAAAGTTGAACG AGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATA AAAAACAGACTACATAATACTGTAAAACACAACATATGCAGTCACTATGA ATCAACTACTTAGATGGTATTAGTGACCTGTA >attR1 CAAGTTTGTACAAAAAAGTTGAACGAGAAACGTAAAATGATATAAATATC AATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAA AACACAACATATGCAGTCACTATGAATCAACTACTTAGATGGTATTAGTG ACCTGTA >attR2 TACAGGTCACTAATACCATCTAAGTAGTTGATTCATAGTGACTGCATATG TTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTA ATATATTGATATTTATATCATTTTACGTTTCTCGTTCAACTTTCTTGTAC AAAGTGG >attR3 CCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTT TATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCT CGTTCAACTTTATTATACATAGTTG >attR4 CAACTTTGTATAGAAAAGTTGAACGAGAAACGTAAAATGATATAAATATC AATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAA AACACAACATATCCAGTCACTATGG