From Tol2Kit
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== Principles of Gateway recombination == | == Principles of Gateway recombination == | ||
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| + | Here's a diagram of a Gatway BP reaction, showing an example of how a "middle" clone (pME) is constructed using PCR with gene-specific primers to add ''att'' sites, then recombination with a donor vector. | ||
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Here's a diagram of a multisite Gateway LR reaction, combining three entry clones (p5E, pME, p3E) with the destination vector pDestTol2pA. | Here's a diagram of a multisite Gateway LR reaction, combining three entry clones (p5E, pME, p3E) with the destination vector pDestTol2pA. | ||
[[Image:LR_reaction.png|LR reaction]] | [[Image:LR_reaction.png|LR reaction]] | ||
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== How we use this in the Tol2kit == | == How we use this in the Tol2kit == | ||
Revision as of 12:39, 2 February 2007
(under construction. 1/31/07)
Principles of Gateway recombination
Here's a diagram of a Gatway BP reaction, showing an example of how a "middle" clone (pME) is constructed using PCR with gene-specific primers to add att sites, then recombination with a donor vector.
Here's a diagram of a multisite Gateway LR reaction, combining three entry clones (p5E, pME, p3E) with the destination vector pDestTol2pA.
