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Revision as of 12:40, 2 February 2007 by Chi-bin (talk | contribs) (Principles of Gateway recombination)

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(under construction. 1/31/07)

Principles of Gateway recombination

Here's a diagram of a Gatway BP reaction, showing an example of how a "middle" clone (pME) is constructed using PCR with gene-specific primers to add att sites, then recombination with a donor vector.

BP reaction


Here's a diagram of a multisite Gateway LR reaction, combining three entry clones (p5E, pME, p3E) with the destination vector pDestTol2pA.

LR reaction

How we use this in the Tol2kit