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Revision as of 19:01, 11 October 2007 by Chi-bin (talk | contribs) (link to archived copy of Invitrogen manual)

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Here is an archived copy of the Invitrogen 3-insert multisite Gateway manual, version D, 3-07-07. We originally had a live link to the Invitrogen site, but after this link broke it seemed easier to hard-archive a copy.

Invitrogen manual version D

Note that Invitrogen seems to be continually tinkering with both att sequences and nomenclature. For instance, the attB2 and attB2R primers switch names between version D and version E. The Tol2kit was originally constructed based on the version C manual; version D seems to be very similar with minor improvements to protocols. We have stuck with the version C/D nomenclature, rather than trying to keep up to date with their changes. Presumably att sites from different generations of the kits are still functionally compatible.