From Tol2Kit
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p3E-IRES-nlsEGFPpA was made by fusion PCR of the EMCV IRES sequence, and nlsEGFP-SV40 late polyA, followed by a BP reaction. Sequencing confirms that all bases of the coding sequence are correct. There is a single base change in the IRES sequence (G481A), but we have confirmed functionally that this cassette produces fluorescent, nuclear-localized protein. | p3E-IRES-nlsEGFPpA was made by fusion PCR of the EMCV IRES sequence, and nlsEGFP-SV40 late polyA, followed by a BP reaction. Sequencing confirms that all bases of the coding sequence are correct. There is a single base change in the IRES sequence (G481A), but we have confirmed functionally that this cassette produces fluorescent, nuclear-localized protein. | ||
| − | === | + | === Sequence === |
| + | Annotated sequence, Genbank format:<br> | ||
| + | [[p3E-IRES-nlsEGFPpA Genbank]] | ||
| + | |||
FASTA file with the full-length sequence as well as sequences of individual components:<br> | FASTA file with the full-length sequence as well as sequences of individual components:<br> | ||
[[p3E-IRES-nlsEGFPpA sequence]] | [[p3E-IRES-nlsEGFPpA sequence]] | ||
Revision as of 03:54, 19 December 2007
Construction details
p3E-IRES-nlsEGFPpA was made by fusion PCR of the EMCV IRES sequence, and nlsEGFP-SV40 late polyA, followed by a BP reaction. Sequencing confirms that all bases of the coding sequence are correct. There is a single base change in the IRES sequence (G481A), but we have confirmed functionally that this cassette produces fluorescent, nuclear-localized protein.
Sequence
Annotated sequence, Genbank format:
p3E-IRES-nlsEGFPpA Genbank
FASTA file with the full-length sequence as well as sequences of individual components:
p3E-IRES-nlsEGFPpA sequence
Crude map
Screenshot from Sequencher showing locations of components:
