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(New page: <pre> >p5E-Fse-Asc CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCAT...)
 
 
Line 55: Line 55:
 
AAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTT
 
AAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTT
 
TTGCTCACATGTT
 
TTGCTCACATGTT
>pDONR_P4-P1R_5'_end
 
CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG
 
AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA
 
GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC
 
GCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGA
 
AAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAA
 
GAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTA
 
GTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGG
 
CCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCA
 
GGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCC
 
GACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGT
 
TAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCC
 
AGTCTTAAGCTCGGGCCCGCGTTAACGCTACCATGGAGCTCCAAATAATG
 
ATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATAAGCA
 
ATGCTTTTTTATAATGCCAACTTTGTATAGAAAAGTTG
 
 
>T2
 
>T2
 
AAAAGGCCATCCGTCAGGATGGCCTTCT
 
AAAAGGCCATCCGTCAGGATGGCCTTCT
Line 87: Line 72:
 
AACACAACATATGCAGTCACTATGAATCAACTACTTAGATGGTATTAGTG
 
AACACAACATATGCAGTCACTATGAATCAACTACTTAGATGGTATTAGTG
 
ACCTGTA
 
ACCTGTA
>pDONR_P4-P1R_3'_end
 
TTTGTACAAAAAAGTTGAACGAGAAACGTAAAATGATATAAATATCAATA
 
TATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACA
 
CAACATATGCAGTCACTATGAATCAACTACTTAGATGGTATTAGTGACCT
 
GTAGAATTCGAGCTCTAGAGCTGCAGGGCGGCCGCGATATCCCCTATAGT
 
GAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTC
 
TCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGAA
 
CAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGC
 
CATATTCAACGGGAAACGTCGAGGCCGCGATTAAATTCCAACATGGATGC
 
TGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTG
 
CGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTG
 
AAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAG
 
ACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTA
 
TCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGAAAA
 
ACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGT
 
TGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTA
 
ATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCA
 
CGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAA
 
TGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCAT
 
TCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTT
 
ATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGG
 
AATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTG
 
AGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGAT
 
AATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTT
 
CTAATCAGAATTGGTTAATTGGTTGTAACACTGGCAGAGCATTACGCTGA
 
CTTGACGGGACGGCGCAAGCTCATGACCAAAATCCCTTAACGTGAGTTAC
 
GCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTC
 
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAAC
 
CACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTT
 
TTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCT
 
TCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGC
 
CTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGC
 
GATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAA
 
GGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGG
 
AGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAA
 
AGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGG
 
CAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCT
 
GGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGA
 
TTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAA
 
CGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGT
 
T
 
 
>T7
 
>T7
 
TACGACTCACTATAGGGGAT
 
TACGACTCACTATAGGGGAT

Latest revision as of 23:13, 19 March 2007

>p5E-Fse-Asc
CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG
AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA
GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC
GCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGA
AAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAA
GAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTA
GTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGG
CCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCA
GGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCC
GACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGT
TAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCC
AGTCTTAAGCTCGGGCCCGCGTTAACGCTACCATGGAGCTCCAAATAATG
ATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATAAGCA
ATGCTTTTTTATAATGCCAACTTTGTATAGAAAAGTTGGGCCGGCCCTTC
GGCGCGCCCAAGTTTGTACAAAAAAGTTGAACGAGAAACGTAAAATGATA
TAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAA
TACTGTAAAACACAACATATGCAGTCACTATGAATCAACTACTTAGATGG
TATTAGTGACCTGTAGAATTCGAGCTCTAGAGCTGCAGGGCGGCCGCGAT
ATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCT
CTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATA
TATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGG
GGTGTTATGAGCCATATTCAACGGGAAACGTCGAGGCCGCGATTAAATTC
CAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCG
GGCAATCAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCA
GAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGA
TGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCA
TCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCG
ATCCCCGGAAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGG
TGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGA
TTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCT
CAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGA
TGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATA
AACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCA
CTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGT
TGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGA
ACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAA
TATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCT
CGATGAGTTTTTCTAATCAGAATTGGTTAATTGGTTGTAACACTGGCAGA
GCATTACGCTGACTTGACGGGACGGCGCAAGCTCATGACCAAAATCCCTT
AACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGAT
CAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGC
AAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAG
CTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACC
AAATACTGTYCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACT
CTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCT
GCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATA
GTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACAC
AGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGT
GAGCWWTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTA
TCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAG
GGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGA
CTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAA
AAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTT
TTGCTCACATGTT
>T2
AAAAGGCCATCCGTCAGGATGGCCTTCT
>T1
ATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTAT
>M13_Forward
GTAAAACGACGGCCAG
>attL4
AAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATT
GATAAGCAATGCTTTTTTATAATGCCAACTTTGTATAGAAAAGTTG
>p5E-Fse-Asc_insert
CAACTTTGTATAGAAAAGTTGGGCCGGCCCTTCGGCGCGCCCAAGTTTGT
ACAAAAAAG
>attR1
CAAGTTTGTACAAAAAAGTTGAACGAGAAACGTAAAATGATATAAATATC
AATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAA
AACACAACATATGCAGTCACTATGAATCAACTACTTAGATGGTATTAGTG
ACCTGTA
>T7
TACGACTCACTATAGGGGAT
>M13_Reverse
CAGGAAACAGCTATGAC
>kan
ATGAGCCATATTCAACGGGAAACGTCGAGGCCGCGATTAAATTCCAACAT
GGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAAT
CAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTTG
TTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGAT
GGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGC
ATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCC
GGAAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAA
TATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTG
TTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCG
CAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGA
GCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTT
TGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGAT
AACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACG
AGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCC
TCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGT
ATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGA
GTTTTTCTAA
>pUC_ori
CCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGT
AATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTT
TGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGC
AGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCA
CCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCC
TGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTG
GACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGG
GGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGA
GATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGA
AAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCAC
GAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGT
TTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGG
CGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGC
CTTTTGCTGGCCTTTTGCTCACAT