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LOCUS      396.pCS2-transposase        6034 bp ds-DNA    circular    18-DEC-2007
 +
REFERENCE  1
 +
  AUTHORS  Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS,
 +
Parant JM, Yost HJ, Kanki JP, Chien CB.
 +
  TITLE    The Tol2kit: a multisite gateway-based construction kit for Tol2
 +
transposon transgenesis constructs.
 +
  JOURNAL  Developmental Dynamics 236(11):3088-99 (2007).
 +
  PUBMED    17937395
 +
COMMENT    396.pCS2-transposase, Tol2kit v1.0
 +
COMMENT    ApEinfo:methylated:1
 +
FEATURES            Location/Qualifiers
 +
    polyA_signal    3070..3267
 +
                    /label=SV40 late polyA (from pCS2+)
 +
                    /ApEinfo_fwdcolor=#ffff7f
 +
                    /ApEinfo_revcolor=#ffff7f
 +
    promoter        10..987
 +
                    /label=sCMV IE94
 +
                    /ApEinfo_fwdcolor=#ffffbf
 +
                    /ApEinfo_revcolor=#ffffbf
 +
    rep_origin      complement(3699..4372)
 +
                    /label=pUC ori
 +
                    /ApEinfo_fwdcolor=#999999
 +
                    /ApEinfo_revcolor=#999999
 +
    rep_origin      complement(3740..4422)
 +
                    /label=colE1 ori
 +
                    /ApEinfo_fwdcolor=#999999
 +
                    /ApEinfo_revcolor=#999999
 +
    rep_origin      complement(5640..5946)
 +
                    /label=f1(+) ori
 +
                    /ApEinfo_fwdcolor=#999999
 +
                    /ApEinfo_revcolor=#999999
 +
    CDS            complement(4520..5377)
 +
                    /label=ampR
 +
                    /ApEinfo_fwdcolor=#ff7f00
 +
                    /ApEinfo_revcolor=#ff7f00
 +
    primer_bind    complement(3351..3372)
 +
                    /label=M13R
 +
                    /ApEinfo_fwdcolor=#777777
 +
                    /ApEinfo_revcolor=#777777
 +
    promoter        complement(3316..3335)
 +
                    /label=T3
 +
                    /ApEinfo_fwdcolor=#777777
 +
                    /ApEinfo_revcolor=#777777
 +
    promoter        1022..1038
 +
                    /label=SP6
 +
                    /ApEinfo_fwdcolor=#ffff00
 +
                    /ApEinfo_revcolor=#ffff00
 +
    misc_feature    1083..3032
 +
                    /label=Tol2 transposase cds
 +
                    /ApEinfo_fwdcolor=cyan
 +
                    /ApEinfo_revcolor=green
 +
ORIGIN
 +
        1 TCGACCATAG CCAATTCAAT ATGGCGTATA TGGACTCATG CCAATTCAAT ATGGTGGATC
 +
      61 TGGACCTGTG CCAATTCAAT ATGGCGTATA TGGACTCGTG CCAATTCAAT ATGGTGGATC
 +
      121 TGGACCCCAG CCAATTCAAT ATGGCGGACT TGGCACCATG CCAATTCAAT ATGGCGGACT
 +
      181 TGGCACTGTG CCAACTGGGG AGGGGTCTAC TTGGCACGGT GCCAAGTTTG AGGAGGGGTC
 +
      241 TTGGCCCTGT GCCAAGTCCG CCATATTGAA TTGGCATGGT GCCAATAATG GCGGCCATAT
 +
      301 TGGCTATATG CCAGGATCAA TATATAGGCA ATATCCAATA TGGCCCTATG CCAATATGGC
 +
      361 TATTGGCCAG GTTCAATACT ATGTATTGGC CCTATGCCAT ATAGTATTCC ATATATGGGT
 +
      421 TTTCCTATTG ACGTAGATAG CCCCTCCCAA TGGGCGGTCC CATATACCAT ATATGGGGCT
 +
      481 TCCTAATACC GCCCATAGCC ACTCCCCCAT TGACGTCAAT GGTCTCTATA TATGGTCTTT
 +
      541 CCTATTGACG TCATATGGGC GGTCCTATTG ACGTATATGG CGCCTCCCCC ATTGACGTCA
 +
      601 ATTACGGTAA ATGGCCCGCC TGGCTCAATG CCCATTGACG TCAATAGGAC CACCCACCAT
 +
      661 TGACGTCAAT GGGATGGCTC ATTGCCCATT CATATCCGTT CTCACGCCCC CTATTGACGT
 +
      721 CAATGACGGT AAATGGCCCA CTTGGCAGTA CATCAATATC TATTAATAGT AACTTGGCAA
 +
      781 GTACATTACT ATTGGAAGTA CGCCAGGGTA CATTGGCAGT ACTCCCATTG ACGTCAATGG
 +
      841 CGGTAAATGG CCCGCGATGG CTGCCAAGTA CATCCCCATT GACGTCAATG GGGAGGGGCA
 +
      901 ATGACGCAAA TGGGCGTTCC ATTGACGTAA ATGGGCGGTA GGCGTGCCTA ATGGGAGGTC
 +
      961 TATATAAGCA ATGCTCGTTT AGGGAACCGC CATTCTGCCT GGGGACGTCG GAGCAAGCTT
 +
    1021 GATTTAGGTG ACACTATAGA ATACAAGCTA CTTGTTCTTT TTGCAGGATC CGGCCGGCCA
 +
    1081 CCATGGAGGA AGTATGTGAT TCATCAGCAG CTGCGAGCAG CACAGTCCAA AATCAGCCAC
 +
    1141 AGGATCAAGA GCACCCGTGG CCGTATCTTC GCGAATTCTT TTCTTTAAGT GGTGTAAATA
 +
    1201 AAGATTCATT CAAGATGAAA TGTGTCCTCT GTCTCCCGCT TAATAAAGAA ATATCGGCCT
 +
    1261 TCAAAAGTTC GCCATCAAAC CTAAGGAAGC ATATTGAGAG AATGCACCCA AATTACCTCA
 +
    1321 AAAACTACTC TAAATTGACA GCACAGAAGA GAAAGATCGG GACCTCCACC CATGCTTCCA
 +
    1381 GCAGTAAGCA ACTGAAAGTT GACTCAGTTT TCCCAGTCAA ACATGTGTCT CCAGTCACTG
 +
    1441 TGAACAAAGC TATATTAAGG TACATCATTC AAGGACTTCA TCCTTTCAGC ACTGTTGATC
 +
    1501 TGCCATCATT TAAAGAGCTG ATTAGTACAC TGCAGCCTGG CATTTCTGTC ATTACAAGGC
 +
    1561 CTACTTTACG CTCCAAGATA GCTGAAGCTG CTCTGATCAT GAAACAGAAA GTGACTGCTG
 +
    1621 CCATGAGTGA AGTTGAATGG ATTGCAACCA CAACGGATTG TTGGACTGCA CGTAGAAAGT
 +
    1681 CATTCATTGG TGTAACTGCT CACTGGATCA ACCCTGGAAG TCTTGAAAGA CATTCCGCTG
 +
    1741 CACTTGCCTG CAAAAGATTA ATGGGCTCTC ATACTTTTGA GGTACTGGCC AGTGCCATGA
 +
    1801 ATGATATCCA CTCAGAGTAT GAAATACGTG ACAAGGTTGT TTGCACAACC ACAGACAGTG
 +
    1861 GTTCCAACTT TATGAAGGCT TTCAGAGTTT TTGGTGTGGA AAACAATGAT ATCGAGACTG
 +
    1921 AGGCAAGAAG GTGTGAAAGT GATGACACTG ATTCTGAAGG CTGTGGTGAG GGAAGTGATG
 +
    1981 GTGTGGAATT CCAAGATGCC TCACGAGTCC TGGACCAAGA CGATGGCTTC GAATTCCAGC
 +
    2041 TACCAAAACA TCAAAAGTGT GCCTGTCACT TACTTAACCT AGTCTCAAGC GTTGATGCCC
 +
    2101 AAAAAGCTCT CTCAAATGAA CACTACAAGA AACTCTACAG ATCTGTCTTT GGCAAATGCC
 +
    2161 AAGCTTTATG GAATAAAAGC AGCCGATCGG CTCTAGCAGC TGAAGCTGTT GAATCAGAAA
 +
    2221 GCCGGCTTCA GCTTTTAAGG CCAAACCAAA CGCGGTGGAA TTCAACTTTT ATGGCTGTTG
 +
    2281 ACAGAATTCT TCAAATTTGC AAAGAAGCAG GAGAAGGCGC ACTTCGGAAT ATATGCACCT
 +
    2341 CTCTTGAGGT TCCAATGTTT AATCCAGCAG AAATGCTGTT CTTGACAGAG TGGGCCAACA
 +
    2401 CAATGCGTCC AGTTGCAAAA GTACTCGACA TCTTGCAAGC GGAAACGAAT ACACAGCTGG
 +
    2461 GGTGGCTGCT GCCTAGTGTC CATCAGTTAA GCTTGAAACT TCAGCGACTC CACCATTCTC
 +
    2521 TCAGGTACTG TGACCCACTT GTGGATGCCC TACAACAAGG AATCCAAACA CGATTCAAGC
 +
    2581 ATATGTTTGA AGATCCTGAG ATCATAGCAG CTGCCATCCT TCTCCCTAAA TTTCGGACCT
 +
    2641 CTTGGACAAA TGATGAAACC ATCATAAAAC GAGGCATGGA CTACATCAGA GTGCATCTGG
 +
    2701 AGCCTTTGGA CCACAAGAAG GAATTGGCCA ACAGTTCATC TGATGATGAA GATTTTTTCG
 +
    2761 CTTCTTTGAA ACCGACAACA CATGAAGCCA GCAAAGAGTT GGATGGATAT CTGGCCTGTG
 +
    2821 TTTCAGACAC CAGGGAGTCT CTGCTCACGT TTCCTGCTAT TTGCAGCCTC TCTATCAAGA
 +
    2881 CTAATACACC TCTTCCCGCA TCGGCTGCCT GTGAGAGGCT TTTCAGCACT GCAGGATTGC
 +
    2941 TTTTCAGCCC CAAAAGAGCT AGGCTTGACA CTAACAATTT TGAGAATCAG CTTCTACTGA
 +
    3001 AGTTAAATCT GAGGTTTTAC AACTTTGAGT AGGGCGCGCC TCTAGAACTA TAGTGAGTCG
 +
    3061 TATTACGTAG ATCCAGACAT GATAAGATAC ATTGATGAGT TTGGACAAAC CACAACTAGA
 +
    3121 ATGCAGTGAA AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT ATTTGTAACC
 +
    3181 ATTATAAGCT GCAATAAACA AGTTAACAAC AACAATTGCA TTCATTTTAT GTTTCAGGTT
 +
    3241 CAGGGGGAGG TGTGGGAGGT TTTTTAATTC GCGGCCGCGG CGCCAATGCA TTGGGCCCGG
 +
    3301 TACCCAGCTT TTGTTCCCTT TAGTGAGGGT TAATTGCGCG CTTGGCGTAA TCATGGTCAT
 +
    3361 AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA
 +
    3421 GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC
 +
    3481 GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC
 +
    3541 AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT
 +
    3601 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC
 +
    3661 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA
 +
    3721 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG
 +
    3781 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA
 +
    3841 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC
 +
    3901 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC
 +
    3961 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC
 +
    4021 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG
 +
    4081 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT
 +
    4141 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA
 +
    4201 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT
 +
    4261 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA
 +
    4321 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG
 +
    4381 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT
 +
    4441 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT
 +
    4501 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC
 +
    4561 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG
 +
    4621 GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG
 +
    4681 ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT
 +
    4741 TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG
 +
    4801 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT
 +
    4861 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA
 +
    4921 TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG
 +
    4981 CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT
 +
    5041 CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA
 +
    5101 TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA
 +
    5161 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT
 +
    5221 TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT
 +
    5281 CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA
 +
    5341 AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT
 +
    5401 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA
 +
    5461 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTAAA TTGTAAGCGT
 +
    5521 TAATATTTTG TTAAAATTCG CGTTAAATTT TTGTTAAATC AGCTCATTTT TTAACCAATA
 +
    5581 GGCCGAAATC GGCAAAATCC CTTATAAATC AAAAGAATAG ACCGAGATAG GGTTGAGTGT
 +
    5641 TGTTCCAGTT TGGAACAAGA GTCCACTATT AAAGAACGTG GACTCCAACG TCAAAGGGCG
 +
    5701 AAAAACCGTC TATCAGGGCG ATGGCCCACT ACGTGAACCA TCACCCTAAT CAAGTTTTTT
 +
    5761 GGGGTCGAGG TGCCGTAAAG CACTAAATCG GAACCCTAAA GGGAGCCCCC GATTTAGAGC
 +
    5821 TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG AAGAAAGCGA AAGGAGCGGG
 +
    5881 CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA ACCACCACAC CCGCCGCGCT
 +
    5941 TAATGCGCCG CTACAGGGCG CGTCCCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG
 +
    6001 GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAG 
 +
//
 
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</pre>

Latest revision as of 04:18, 19 December 2007

LOCUS       396.pCS2-transposase        6034 bp ds-DNA     circular     18-DEC-2007
REFERENCE   1
  AUTHORS   Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, 
			Parant JM, Yost HJ, Kanki JP, Chien CB.
  TITLE     The Tol2kit: a multisite gateway-based construction kit for Tol2 
			transposon transgenesis constructs.
  JOURNAL   Developmental Dynamics 236(11):3088-99 (2007).
  PUBMED    17937395
COMMENT     396.pCS2-transposase, Tol2kit v1.0
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     polyA_signal    3070..3267
                     /label=SV40 late polyA (from pCS2+)
                     /ApEinfo_fwdcolor=#ffff7f
                     /ApEinfo_revcolor=#ffff7f
     promoter        10..987
                     /label=sCMV IE94
                     /ApEinfo_fwdcolor=#ffffbf
                     /ApEinfo_revcolor=#ffffbf
     rep_origin      complement(3699..4372)
                     /label=pUC ori
                     /ApEinfo_fwdcolor=#999999
                     /ApEinfo_revcolor=#999999
     rep_origin      complement(3740..4422)
                     /label=colE1 ori
                     /ApEinfo_fwdcolor=#999999
                     /ApEinfo_revcolor=#999999
     rep_origin      complement(5640..5946)
                     /label=f1(+) ori
                     /ApEinfo_fwdcolor=#999999
                     /ApEinfo_revcolor=#999999
     CDS             complement(4520..5377)
                     /label=ampR
                     /ApEinfo_fwdcolor=#ff7f00
                     /ApEinfo_revcolor=#ff7f00
     primer_bind     complement(3351..3372)
                     /label=M13R
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
     promoter        complement(3316..3335)
                     /label=T3
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
     promoter        1022..1038
                     /label=SP6
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=#ffff00
     misc_feature    1083..3032
                     /label=Tol2 transposase cds
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
ORIGIN
        1 TCGACCATAG CCAATTCAAT ATGGCGTATA TGGACTCATG CCAATTCAAT ATGGTGGATC
       61 TGGACCTGTG CCAATTCAAT ATGGCGTATA TGGACTCGTG CCAATTCAAT ATGGTGGATC
      121 TGGACCCCAG CCAATTCAAT ATGGCGGACT TGGCACCATG CCAATTCAAT ATGGCGGACT
      181 TGGCACTGTG CCAACTGGGG AGGGGTCTAC TTGGCACGGT GCCAAGTTTG AGGAGGGGTC
      241 TTGGCCCTGT GCCAAGTCCG CCATATTGAA TTGGCATGGT GCCAATAATG GCGGCCATAT
      301 TGGCTATATG CCAGGATCAA TATATAGGCA ATATCCAATA TGGCCCTATG CCAATATGGC
      361 TATTGGCCAG GTTCAATACT ATGTATTGGC CCTATGCCAT ATAGTATTCC ATATATGGGT
      421 TTTCCTATTG ACGTAGATAG CCCCTCCCAA TGGGCGGTCC CATATACCAT ATATGGGGCT
      481 TCCTAATACC GCCCATAGCC ACTCCCCCAT TGACGTCAAT GGTCTCTATA TATGGTCTTT
      541 CCTATTGACG TCATATGGGC GGTCCTATTG ACGTATATGG CGCCTCCCCC ATTGACGTCA
      601 ATTACGGTAA ATGGCCCGCC TGGCTCAATG CCCATTGACG TCAATAGGAC CACCCACCAT
      661 TGACGTCAAT GGGATGGCTC ATTGCCCATT CATATCCGTT CTCACGCCCC CTATTGACGT
      721 CAATGACGGT AAATGGCCCA CTTGGCAGTA CATCAATATC TATTAATAGT AACTTGGCAA
      781 GTACATTACT ATTGGAAGTA CGCCAGGGTA CATTGGCAGT ACTCCCATTG ACGTCAATGG
      841 CGGTAAATGG CCCGCGATGG CTGCCAAGTA CATCCCCATT GACGTCAATG GGGAGGGGCA
      901 ATGACGCAAA TGGGCGTTCC ATTGACGTAA ATGGGCGGTA GGCGTGCCTA ATGGGAGGTC
      961 TATATAAGCA ATGCTCGTTT AGGGAACCGC CATTCTGCCT GGGGACGTCG GAGCAAGCTT
     1021 GATTTAGGTG ACACTATAGA ATACAAGCTA CTTGTTCTTT TTGCAGGATC CGGCCGGCCA
     1081 CCATGGAGGA AGTATGTGAT TCATCAGCAG CTGCGAGCAG CACAGTCCAA AATCAGCCAC
     1141 AGGATCAAGA GCACCCGTGG CCGTATCTTC GCGAATTCTT TTCTTTAAGT GGTGTAAATA
     1201 AAGATTCATT CAAGATGAAA TGTGTCCTCT GTCTCCCGCT TAATAAAGAA ATATCGGCCT
     1261 TCAAAAGTTC GCCATCAAAC CTAAGGAAGC ATATTGAGAG AATGCACCCA AATTACCTCA
     1321 AAAACTACTC TAAATTGACA GCACAGAAGA GAAAGATCGG GACCTCCACC CATGCTTCCA
     1381 GCAGTAAGCA ACTGAAAGTT GACTCAGTTT TCCCAGTCAA ACATGTGTCT CCAGTCACTG
     1441 TGAACAAAGC TATATTAAGG TACATCATTC AAGGACTTCA TCCTTTCAGC ACTGTTGATC
     1501 TGCCATCATT TAAAGAGCTG ATTAGTACAC TGCAGCCTGG CATTTCTGTC ATTACAAGGC
     1561 CTACTTTACG CTCCAAGATA GCTGAAGCTG CTCTGATCAT GAAACAGAAA GTGACTGCTG
     1621 CCATGAGTGA AGTTGAATGG ATTGCAACCA CAACGGATTG TTGGACTGCA CGTAGAAAGT
     1681 CATTCATTGG TGTAACTGCT CACTGGATCA ACCCTGGAAG TCTTGAAAGA CATTCCGCTG
     1741 CACTTGCCTG CAAAAGATTA ATGGGCTCTC ATACTTTTGA GGTACTGGCC AGTGCCATGA
     1801 ATGATATCCA CTCAGAGTAT GAAATACGTG ACAAGGTTGT TTGCACAACC ACAGACAGTG
     1861 GTTCCAACTT TATGAAGGCT TTCAGAGTTT TTGGTGTGGA AAACAATGAT ATCGAGACTG
     1921 AGGCAAGAAG GTGTGAAAGT GATGACACTG ATTCTGAAGG CTGTGGTGAG GGAAGTGATG
     1981 GTGTGGAATT CCAAGATGCC TCACGAGTCC TGGACCAAGA CGATGGCTTC GAATTCCAGC
     2041 TACCAAAACA TCAAAAGTGT GCCTGTCACT TACTTAACCT AGTCTCAAGC GTTGATGCCC
     2101 AAAAAGCTCT CTCAAATGAA CACTACAAGA AACTCTACAG ATCTGTCTTT GGCAAATGCC
     2161 AAGCTTTATG GAATAAAAGC AGCCGATCGG CTCTAGCAGC TGAAGCTGTT GAATCAGAAA
     2221 GCCGGCTTCA GCTTTTAAGG CCAAACCAAA CGCGGTGGAA TTCAACTTTT ATGGCTGTTG
     2281 ACAGAATTCT TCAAATTTGC AAAGAAGCAG GAGAAGGCGC ACTTCGGAAT ATATGCACCT
     2341 CTCTTGAGGT TCCAATGTTT AATCCAGCAG AAATGCTGTT CTTGACAGAG TGGGCCAACA
     2401 CAATGCGTCC AGTTGCAAAA GTACTCGACA TCTTGCAAGC GGAAACGAAT ACACAGCTGG
     2461 GGTGGCTGCT GCCTAGTGTC CATCAGTTAA GCTTGAAACT TCAGCGACTC CACCATTCTC
     2521 TCAGGTACTG TGACCCACTT GTGGATGCCC TACAACAAGG AATCCAAACA CGATTCAAGC
     2581 ATATGTTTGA AGATCCTGAG ATCATAGCAG CTGCCATCCT TCTCCCTAAA TTTCGGACCT
     2641 CTTGGACAAA TGATGAAACC ATCATAAAAC GAGGCATGGA CTACATCAGA GTGCATCTGG
     2701 AGCCTTTGGA CCACAAGAAG GAATTGGCCA ACAGTTCATC TGATGATGAA GATTTTTTCG
     2761 CTTCTTTGAA ACCGACAACA CATGAAGCCA GCAAAGAGTT GGATGGATAT CTGGCCTGTG
     2821 TTTCAGACAC CAGGGAGTCT CTGCTCACGT TTCCTGCTAT TTGCAGCCTC TCTATCAAGA
     2881 CTAATACACC TCTTCCCGCA TCGGCTGCCT GTGAGAGGCT TTTCAGCACT GCAGGATTGC
     2941 TTTTCAGCCC CAAAAGAGCT AGGCTTGACA CTAACAATTT TGAGAATCAG CTTCTACTGA
     3001 AGTTAAATCT GAGGTTTTAC AACTTTGAGT AGGGCGCGCC TCTAGAACTA TAGTGAGTCG
     3061 TATTACGTAG ATCCAGACAT GATAAGATAC ATTGATGAGT TTGGACAAAC CACAACTAGA
     3121 ATGCAGTGAA AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT ATTTGTAACC
     3181 ATTATAAGCT GCAATAAACA AGTTAACAAC AACAATTGCA TTCATTTTAT GTTTCAGGTT
     3241 CAGGGGGAGG TGTGGGAGGT TTTTTAATTC GCGGCCGCGG CGCCAATGCA TTGGGCCCGG
     3301 TACCCAGCTT TTGTTCCCTT TAGTGAGGGT TAATTGCGCG CTTGGCGTAA TCATGGTCAT
     3361 AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA
     3421 GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC
     3481 GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC
     3541 AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT
     3601 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC
     3661 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA
     3721 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG
     3781 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA
     3841 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC
     3901 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC
     3961 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC
     4021 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG
     4081 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT
     4141 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA
     4201 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT
     4261 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA
     4321 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG
     4381 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT
     4441 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT
     4501 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC
     4561 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG
     4621 GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG
     4681 ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT
     4741 TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG
     4801 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT
     4861 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA
     4921 TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG
     4981 CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT
     5041 CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA
     5101 TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA
     5161 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT
     5221 TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT
     5281 CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA
     5341 AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT
     5401 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA
     5461 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTAAA TTGTAAGCGT
     5521 TAATATTTTG TTAAAATTCG CGTTAAATTT TTGTTAAATC AGCTCATTTT TTAACCAATA
     5581 GGCCGAAATC GGCAAAATCC CTTATAAATC AAAAGAATAG ACCGAGATAG GGTTGAGTGT
     5641 TGTTCCAGTT TGGAACAAGA GTCCACTATT AAAGAACGTG GACTCCAACG TCAAAGGGCG
     5701 AAAAACCGTC TATCAGGGCG ATGGCCCACT ACGTGAACCA TCACCCTAAT CAAGTTTTTT
     5761 GGGGTCGAGG TGCCGTAAAG CACTAAATCG GAACCCTAAA GGGAGCCCCC GATTTAGAGC
     5821 TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG AAGAAAGCGA AAGGAGCGGG
     5881 CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA ACCACCACAC CCGCCGCGCT
     5941 TAATGCGCCG CTACAGGGCG CGTCCCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG
     6001 GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAG  
//