From Tol2Kit
(→Construction details) |
(→Crudely annotated sequence) |
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FASTA file with the full-length sequence as well as sequences of individual components):<br> | FASTA file with the full-length sequence as well as sequences of individual components):<br> | ||
[[pDestTol2pA2 sequence]] | [[pDestTol2pA2 sequence]] | ||
| + | |||
| + | Edited 7 June 2007 to add six bases (a KpnI site that was missing). | ||
=== Crude map === | === Crude map === | ||
Screenshot from Sequencher showing locations of components:<br> | Screenshot from Sequencher showing locations of components:<br> | ||
[[Image:pDestTol2pA2.png]] | [[Image:pDestTol2pA2.png]] | ||
Revision as of 17:47, 7 June 2007
Construction details
pDestTol2pA2 was made from pDestTol2pA by cutting with AfeI/DraIII and religating; this removed ~2kb comprising medaka tyrosinase genomic sequence and part of the f1 ori. This reduced the size of the construct without affecting its functionality.
Crudely annotated sequence
FASTA file with the full-length sequence as well as sequences of individual components):
pDestTol2pA2 sequence
Edited 7 June 2007 to add six bases (a KpnI site that was missing).
Crude map
Screenshot from Sequencher showing locations of components:
