From Tol2Kit
Revision as of 19:00, 7 February 2007 by Chi-bin (talk | contribs) (New page: '''Construction details'''<br> pDestTol2pA2 was made from pDestTol2pA by cutting with AfeII/DraIII and religating; this removed ~2kb comprising medaka tyrosinase genomic sequence and part ...)
Construction details
pDestTol2pA2 was made from pDestTol2pA by cutting with AfeII/DraIII and religating; this removed ~2kb comprising medaka tyrosinase genomic sequence and part of the f1 ori. This reduced the size of the construct without affecting its functionality.
Crudely annotated sequence
FASTA file with the full-length sequence as well as sequences of individual components):
pDestTol2pA sequence
Crude map
Screenshot from Sequencher showing locations of components: