From Tol2Kit
m (PDestTol2pA sequence moved to PDestTol2pA2 sequence: typo in original name) |
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| (One intermediate revision by the same user not shown) | |||
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<pre> | <pre> | ||
| − | > | + | >pDestTol2pA2 |
CCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTT | CCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTT | ||
TGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCC | TGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCC | ||
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GCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATC | GCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATC | ||
ACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGT | ACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGT | ||
| − | + | AAACCTTAAACTGCATTTCACCAGCCCCTGTTCTCGTCGGCAAAGGAGCC | |
GTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCG | GTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCG | ||
CTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTG | CTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTG | ||
| Line 57: | Line 57: | ||
TTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATT | TTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATT | ||
GATATTTATATCATTTTACGTTTCTCGTTCAACTTTATTATACATAGTTG | GATATTTATATCATTTTACGTTTCTCGTTCAACTTTATTATACATAGTTG | ||
| − | + | ATAATTCACTGGCCGTCGTTTTACGGTACCATCGATGATGATCCAGACAT | |
| − | + | GATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAA | |
| − | + | AAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACC | |
| − | + | ATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTAT | |
| − | + | GTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACC | |
| − | + | TCTACAAATGTGGTATGGCTGATTATGATCCTCTAGATCAGATCTGCGAA | |
| − | + | GATACGGCCACGGGTGCTCTTGATCCTGTGGCTGATTTTGGACTGTGCTG | |
| − | + | CTCGCAGCTGCTGATGAATCACATACTTCCTCCATTTTCTTCCACTGATT | |
| − | + | GACTGTTATAATTTCCCTAATTTCCAGGTCAAGGTGCTGTGCATTGTGGT | |
| − | + | AATAGATGTGACATGACGTCACTTCCAAAGGACCAATGAACATGTCTGAC | |
| − | + | CAATTTCATATAATGTGAAAACGATTTTCATAGGCAGAATAAATAACATT | |
| − | + | TAAATTAAACTGGGCATCAGCGCAATTCAATTGGTTTGGTAATAGCAAGG | |
| − | + | GAAAATAGAATGAAGTGATCTCCAAAAAATAAGTACTTTTTGACTGTAAA | |
| − | + | TAAAATTGTAAGGAGTAAAAAGTACTTTTTTTTCTAAAAAAATGTAATTA | |
| − | + | AGTAAAAGTAAAAGTATTGATTTTTAATTGTACTCAAGTAAAGTAAAAAT | |
| − | + | CCCCAAAAATAATACTTAAGTACAGTAATCAAGTAAAATTACTCAAGTAC | |
| − | + | TTTACACCTCTGGTTCTTGACCCCCTACCTTCAGCAAGCCCAGCAGATCC | |
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ACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTCCAGCTTTTGTTCCC | ACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTCCAGCTTTTGTTCCC | ||
TTTAGTGAGGGTTAATTGCGCGCTTGGCGTAATCATGGTCATAGCTGTTT | TTTAGTGAGGGTTAATTGCGCGCTTGGCGTAATCATGGTCATAGCTGTTT | ||
| Line 165: | Line 119: | ||
ATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAA | ATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAA | ||
ATAGGGGTTCCGCGCACATTTCCCCGAAAAGTG | ATAGGGGTTCCGCGCACATTTCCCCGAAAAGTG | ||
| − | > | + | >pBSII_SK+_5’_of_DraIII |
| − | + | CCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTT | |
| − | + | TGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCC | |
| − | + | TTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTT | |
| − | + | GGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGA | |
| − | + | AAAACCGTCTATCAGGGCGATGGCCCAC | |
| − | + | >f1_(+)_ori_fragment | |
| − | + | GTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCC | |
| − | + | ACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAA | |
| − | + | >tyrosinase_genomic_from_AB010101 | |
| − | + | GCTGGCTAAGAACTCATCAGCCTCCCCGGTCCATCTACCCACGTACCAAT | |
| − | + | GCACCAATTGGCCACAATGACGGCTACTACATGGTGCCATTCCTTCCTCT | |
| − | + | TTATAGGAATGGAGACTACCTCCTGTCCAACAAGGCTCTTGGATACGAGT | |
| − | + | ACGCCTACCTGTTGGACCCAGGTCATTGCACAACACCAGAAATGCCCTCT | |
| − | + | GATCTGCAAAAGACGTGAATATCTGTTCAGACACCCATATCCACTCTGTT | |
| − | + | CCACACAGGTCAGAGGTTTGTCCAGGAGTTCTTGAC | |
| − | + | >Tyro_frag_ex4-in4-ex5 | |
| − | + | CAGGTCATTGCACAACACCAGAAATGCCCTCTGATCTGCAAAAGACGTGA | |
| − | + | ATATCTGTTCAGACACCCATATCCACTCTGTTCCACACAGG | |
| − | > | + | >3'_TIR |
| − | + | TACTTTTTACACCTCTG | |
| − | + | >536_bp_of_Tol2_3'_end | |
| − | + | ATCTGGCCTGTGTTTCAGACACCAGGGAGTCTCTGCTCACGTTTCCTGCT | |
| − | + | ATTTGCAGCCTCTCTATCAAGACTAATACACCTCTTCCCGCATCGGCTGC | |
| − | + | CTGTGAGAGGCTTTTCAGCACTGCAGGATTGCTTTTCAGCCCCAAAAGAG | |
| − | + | CTAGGCTTGACACTAACAATTTTGAGAATCAGCTTCTACTGAAGTTAAAT | |
| − | + | CTGAGGTTTTACAACTTTGAGTAGCGTGTACTGGCATTAGATTGTCTGTC | |
| − | + | TTATAGTTTGATAATTAAATACAAACAGTTCTAAAGCAGGATAAAACCTT | |
| − | + | GTATGCATTTCATTTAATGTTTTTTGAGATTAAAAGCTTAAACAAGAATC | |
| − | + | TCTAGTTTTCTTTCTTGCTTTTACTTTTACTTCCTTAATACTCAAGTACA | |
| − | + | ATTTTAATGGAGTACTTTTTTACTTTTACTCAAGTAAGATTCTAGCCAGA | |
| − | + | TACTTTTACTTTTAATTGAGTAAAATTTTCCCTAAGTACTTGTACTTTCA | |
| − | + | CTTGAGTAAAATTTTTGAGTACTTTTTACACCTCTG | |
| − | + | >Tol2_exon4_136-482 | |
| + | ATCTGGCCTGTGTTTCAGACACCAGGGAGTCTCTGCTCACGTTTCCTGCT | ||
| + | ATTTGCAGCCTCTCTATCAAGACTAATACACCTCTTCCCGCATCGGCTGC | ||
| + | CTGTGAGAGGCTTTTCAGCACTGCAGGATTGCTTTTCAGCCCCAAAAGAG | ||
| + | CTAGGCTTGACACTAACAATTTTGAGAATCAGCTTCTACTGAAGTTAAAT | ||
| + | CTGAGGTTTTACAACTTTGAGTAGCGTGTACTGGCATTAGATTGTCTGTC | ||
| + | TTATAGTTTGATAATTAAATACAAACAGTTCTAAAGCAGGATAAAACCTT | ||
| + | GTATGCATTTCATTTAATGTTTTTTGAGATTAAAAGCTTAAACAAGA | ||
>M13R | >M13R | ||
GGAAACAGCTATGACCATGA | GGAAACAGCTATGACCATGA | ||
| − | > | + | >attB4/L4/R4_shared |
| − | + | CAACTTTGTATAGAAAAGTTG | |
| − | > | + | >attR4 |
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CAACTTTGTATAGAAAAGTTGAACGAGAAACGTAAAATGATATAAATATC | CAACTTTGTATAGAAAAGTTGAACGAGAAACGTAAAATGATATAAATATC | ||
AATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAA | AATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAA | ||
| − | + | AACACAACATATCCAGTCACTATGG | |
| − | + | >ccdB | |
| − | + | TTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGC | |
| − | + | GGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTG | |
| − | + | GCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCAC | |
| − | + | CGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCA | |
| − | + | GGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACA | |
| − | + | TCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAA | |
| − | + | CTGCAT | |
| − | + | >ccdB_from_pDEST_R4-R3_(1_mutation) | |
| − | + | ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTT | |
| − | + | TGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGA | |
| − | + | TCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCCCCCGTGAACTT | |
| − | + | TACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGA | |
| − | + | TATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCA | |
| − | + | GCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGA | |
| − | + | ATATAA | |
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>cmR | >cmR | ||
TTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCA | TTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCA | ||
| Line 290: | Line 199: | ||
TTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTT | TTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTT | ||
TTTTCTCCAT | TTTTCTCCAT | ||
| − | > | + | >attR3 |
| − | + | CCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTT | |
| − | + | TATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCT | |
| − | + | CGTTCAACTTTATTATACATAGTTG | |
| − | + | >attB3/L3/R3_shared | |
| − | + | CAACTTTATTATACA | |
| − | + | >M13_-20 | |
| − | + | GTAAAACGACGGCCAGTG | |
| − | > | + | >SV40_late_polyA |
| − | + | GATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAG | |
| − | + | AATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTT | |
| − | + | TATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGC | |
| + | ATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAG | ||
| + | CAAGTAAAACCTCTACAAATGTGGTATGGCTGATTAT | ||
| + | >Tol2_exon1,_bp1-335_(of_461) | ||
| + | ATTTTTTGGAGATCACTTCATTCTATTTTCCCTTGCTATTACCAAACCAA | ||
| + | TTGAATTGCGCTGATGCCCAGTTTAATTTAAATGTTATTTATTCTGCCTA | ||
| + | TGAAAATCGTTTTCACATTATATGAAATTGGTCAGACATGTTCATTGGTC | ||
| + | CTTTGGAAGTGACGTCATGTCACATCTATTACCACAATGCACAGCACCTT | ||
| + | GACCTGGAAATTAGGGAAATTATAACAGTCAATCAGTGGAAGAAAATGGA | ||
| + | GGAAGTATGTGATTCATCAGCAGCTGCGAGCAGCACAGTCCAAAATCAGC | ||
| + | CACAGGATCAAGAGCACCCGTGGCCGTATCTTCGC | ||
| + | >517_bp_of_Tol2_5'_end | ||
| + | CAGAGGTGTAAAGTACTTGAGTAATTTTACTTGATTACTGTACTTAAGTA | ||
| + | TTATTTTTGGGGATTTTTACTTTACTTGAGTACAATTAAAAATCAATACT | ||
| + | TTTACTTTTACTTAATTACATTTTTTTAGAAAAAAAAGTACTTTTTACTC | ||
| + | CTTACAATTTTATTTACAGTCAAAAAGTACTTATTTTTTGGAGATCACTT | ||
| + | CATTCTATTTTCCCTTGCTATTACCAAACCAATTGAATTGCGCTGATGCC | ||
| + | CAGTTTAATTTAAATGTTATTTATTCTGCCTATGAAAATCGTTTTCACAT | ||
| + | TATATGAAATTGGTCAGACATGTTCATTGGTCCTTTGGAAGTGACGTCAT | ||
| + | GTCACATCTATTACCACAATGCACAGCACCTTGACCTGGAAATTAGGGAA | ||
| + | ATTATAACAGTCAATCAGTGGAAGAAAATGGAGGAAGTATGTGATTCATC | ||
| + | AGCAGCTGCGAGCAGCACAGTCCAAAATCAGCCACAGGATCAAGAGCACC | ||
| + | CGTGGCCGTATCTTCGC | ||
| + | >5'_TIR | ||
| + | CAGAGGTGTAAAGTACTTG | ||
| + | >Tyro_frag_ex5 | ||
| + | GATCTGCTGGGCTTGCTGAAGGTAGGGGGTCAAGAAC | ||
| + | >pBSII_SK+_3’_of_SpeI | ||
| + | ACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTCCAGCTTTTGTTCCC | ||
| + | TTTAGTGAGGGTTAATTGCGCGCTTGGCGTAATCATGGTCATAGCTGTTT | ||
| + | CCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGG | ||
| + | AAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACAT | ||
| + | TAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGC | ||
| + | CAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTAT | ||
| + | TGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTC | ||
| + | GGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCC | ||
| + | ACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCA | ||
| + | AAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC | ||
| + | TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGG | ||
| + | CGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTC | ||
| + | CCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCG | ||
| + | CCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGG | ||
| + | TATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGA | ||
| + | ACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG | ||
| + | AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGT | ||
| + | AACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAA | ||
| + | GTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCG | ||
| + | CTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCC | ||
| + | GGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCA | ||
| + | GATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTA | ||
| + | CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTC | ||
| + | ATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATG | ||
| + | AAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTT | ||
| + | ACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGT | ||
| + | TCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGA | ||
| + | GGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCT | ||
| + | CACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAG | ||
| + | CGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTG | ||
| + | TTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACG | ||
| + | TTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATG | ||
| + | GCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCC | ||
| + | CATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCA | ||
| + | GAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCAT | ||
| + | AATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGA | ||
| + | GTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCT | ||
| + | CTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTA | ||
| + | AAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGAT | ||
| + | CTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACT | ||
| + | GATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACA | ||
| + | GGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTG | ||
| + | AATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTT | ||
| + | ATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAA | ||
| + | ATAGGGGTTCCGCGCACATTTCCCCGAAAAGTG | ||
| + | >T3_primer | ||
| + | AATTAACCCTCACTAAAGGG | ||
>M13R | >M13R | ||
GGAAACAGCTATGACCATGA | GGAAACAGCTATGACCATGA | ||
| − | > | + | >pUC_ori |
| − | + | TGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCT | |
| − | + | GGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGAC | |
| − | + | GCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCG | |
| − | + | TTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCT | |
| − | + | TACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC | |
| − | + | ATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAG | |
| − | + | CTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATC | |
| − | + | CGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCAC | |
| − | + | TGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGT | |
| − | + | GCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGAC | |
| − | + | AGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAG | |
| − | + | TTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTT | |
| − | + | TTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGA | |
| − | + | TCCTTTGATCTTTTCTAC | |
| − | + | >amp | |
| − | + | ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATT | |
| − | + | TTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATG | |
| − | + | CTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC | |
| − | + | AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGAT | |
| − | > | + | GAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACG |
| − | + | CCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG | |
| − | + | GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGT | |
| − | + | AAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCA | |
| − | + | ACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG | |
| − | + | CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCT | |
| − | + | GAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAA | |
| − | + | TGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT | |
| − | + | TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACC | |
| − | + | ACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTG | |
| − | + | GAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT | |
| − | + | GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAC | |
| − | + | TATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTA | |
| − | + | AGCATTGG | |
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</pre> | </pre> | ||
Latest revision as of 17:48, 7 June 2007
>pDestTol2pA2 CCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTT TGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCC TTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTT GGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGA AAAACCGTCTATCAGGGCGATGGCCCACGCTGGCTAAGAACTCATCAGCC TCCCCGGTCCATCTACCCACGTACCAATGCACCAATTGGCCACAATGACG GCTACTACATGGTGCCATTCCTTCCTCTTTATAGGAATGGAGACTACCTC CTGTCCAACAAGGCTCTTGGATACGAGTACGCCTACCTGTTGGACCCAGG TCATTGCACAACACCAGAAATGCCCTCTGATCTGCAAAAGACGTGAATAT CTGTTCAGACACCCATATCCACTCTGTTCCACACAGGTCAGAGGTTTGTC CAGGAGTTCTTGACAGAGGTGTAAAAAGTACTCAAAAATTTTACTCAAGT GAAAGTACAAGTACTTAGGGAAAATTTTACTCAATTAAAAGTAAAAGTAT CTGGCTAGAATCTTACTTGAGTAAAAGTAAAAAAGTACTCCATTAAAATT GTACTTGAGTATTAAGGAAGTAAAAGTAAAAGCAAGAAAGAAAACTAGAG ATTCTTGTTTAAGCTTTTAATCTCAAAAAACATTAAATGAAATGCATACA AGGTTTTATCCTGCTTTAGAACTGTTTGTATTTAATTATCAAACTATAAG ACAGACAATCTAATGCCAGTACACGCTACTCAAAGTTGTAAAACCTCAGA TTTAACTTCAGTAGAAGCTGATTCTCAAAATTGTTAGTGTCAAGCCTAGC TCTTTTGGGGCTGAAAAGCAATCCTGCAGTGCTGAAAAGCCTCTCACAGG CAGCCGATGCGGGAAGAGGTGTATTAGTCTTGATAGAGAGGCTGCAAATA GCAGGAAACGTGAGCAGAGACTCCCTGGTGTCTGAAACACAGGCCAGATG GGCCCTCGAGCAGGAAACAGCTATGACCATGATTACGCCAAGCTATCAAC TTTGTATAGAAAAGTTGAACGAGAAACGTAAAATGATATAAATATCAATA TATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACA CAACATATCCAGTCACTATGGTCGACCTGCAGACTGGCTGTGTATAAGGG AGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATG TCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGAC CGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGA TATGCACCACCGGGTAAAGTTCACGGGGGACTTTATCTGACAGCAGACGT GCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATC ACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGT AAACCTTAAACTGCATTTCACCAGCCCCTGTTCTCGTCGGCAAAGGAGCC GTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCG CTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTG CTTACCGAACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAG CTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCATACCTCTT TTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAA ATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATC CTCTAGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCA TTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTG AATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCA TGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCA AAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTC AATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACAT CTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTC CAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGG GTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGA ATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGA TAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATC CAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCT CAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCA GTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGACGG ATCCTAACTCAAAATCCACACATTATACGAGCCGGAAGCATAAAGTGTAA AGCCTGGGGGTGCCTAATGCGGCCGCCATAGTGACTGGATATGTTGTGTT TTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATT GATATTTATATCATTTTACGTTTCTCGTTCAACTTTATTATACATAGTTG ATAATTCACTGGCCGTCGTTTTACGGTACCATCGATGATGATCCAGACAT GATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAA AAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACC ATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTAT GTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACC TCTACAAATGTGGTATGGCTGATTATGATCCTCTAGATCAGATCTGCGAA GATACGGCCACGGGTGCTCTTGATCCTGTGGCTGATTTTGGACTGTGCTG CTCGCAGCTGCTGATGAATCACATACTTCCTCCATTTTCTTCCACTGATT GACTGTTATAATTTCCCTAATTTCCAGGTCAAGGTGCTGTGCATTGTGGT AATAGATGTGACATGACGTCACTTCCAAAGGACCAATGAACATGTCTGAC CAATTTCATATAATGTGAAAACGATTTTCATAGGCAGAATAAATAACATT TAAATTAAACTGGGCATCAGCGCAATTCAATTGGTTTGGTAATAGCAAGG GAAAATAGAATGAAGTGATCTCCAAAAAATAAGTACTTTTTGACTGTAAA TAAAATTGTAAGGAGTAAAAAGTACTTTTTTTTCTAAAAAAATGTAATTA AGTAAAAGTAAAAGTATTGATTTTTAATTGTACTCAAGTAAAGTAAAAAT CCCCAAAAATAATACTTAAGTACAGTAATCAAGTAAAATTACTCAAGTAC TTTACACCTCTGGTTCTTGACCCCCTACCTTCAGCAAGCCCAGCAGATCC ACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTCCAGCTTTTGTTCCC TTTAGTGAGGGTTAATTGCGCGCTTGGCGTAATCATGGTCATAGCTGTTT CCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGG AAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACAT TAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGC CAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTAT TGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTC GGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCC ACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCA AAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGG CGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTC CCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCG CCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGG TATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGA ACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGT AACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAA GTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCG CTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCC GGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCA GATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTA CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTC ATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATG AAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTT ACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGT TCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGA GGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCT CACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAG CGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTG TTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACG TTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATG GCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCC CATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCA GAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCAT AATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGA GTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCT CTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTA AAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGAT CTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACT GATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACA GGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTG AATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTT ATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAA ATAGGGGTTCCGCGCACATTTCCCCGAAAAGTG >pBSII_SK+_5’_of_DraIII CCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTT TGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCC TTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTT GGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGA AAAACCGTCTATCAGGGCGATGGCCCAC >f1_(+)_ori_fragment GTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCC ACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAA >tyrosinase_genomic_from_AB010101 GCTGGCTAAGAACTCATCAGCCTCCCCGGTCCATCTACCCACGTACCAAT GCACCAATTGGCCACAATGACGGCTACTACATGGTGCCATTCCTTCCTCT TTATAGGAATGGAGACTACCTCCTGTCCAACAAGGCTCTTGGATACGAGT ACGCCTACCTGTTGGACCCAGGTCATTGCACAACACCAGAAATGCCCTCT GATCTGCAAAAGACGTGAATATCTGTTCAGACACCCATATCCACTCTGTT CCACACAGGTCAGAGGTTTGTCCAGGAGTTCTTGAC >Tyro_frag_ex4-in4-ex5 CAGGTCATTGCACAACACCAGAAATGCCCTCTGATCTGCAAAAGACGTGA ATATCTGTTCAGACACCCATATCCACTCTGTTCCACACAGG >3'_TIR TACTTTTTACACCTCTG >536_bp_of_Tol2_3'_end ATCTGGCCTGTGTTTCAGACACCAGGGAGTCTCTGCTCACGTTTCCTGCT ATTTGCAGCCTCTCTATCAAGACTAATACACCTCTTCCCGCATCGGCTGC CTGTGAGAGGCTTTTCAGCACTGCAGGATTGCTTTTCAGCCCCAAAAGAG CTAGGCTTGACACTAACAATTTTGAGAATCAGCTTCTACTGAAGTTAAAT CTGAGGTTTTACAACTTTGAGTAGCGTGTACTGGCATTAGATTGTCTGTC TTATAGTTTGATAATTAAATACAAACAGTTCTAAAGCAGGATAAAACCTT GTATGCATTTCATTTAATGTTTTTTGAGATTAAAAGCTTAAACAAGAATC TCTAGTTTTCTTTCTTGCTTTTACTTTTACTTCCTTAATACTCAAGTACA ATTTTAATGGAGTACTTTTTTACTTTTACTCAAGTAAGATTCTAGCCAGA TACTTTTACTTTTAATTGAGTAAAATTTTCCCTAAGTACTTGTACTTTCA CTTGAGTAAAATTTTTGAGTACTTTTTACACCTCTG >Tol2_exon4_136-482 ATCTGGCCTGTGTTTCAGACACCAGGGAGTCTCTGCTCACGTTTCCTGCT ATTTGCAGCCTCTCTATCAAGACTAATACACCTCTTCCCGCATCGGCTGC CTGTGAGAGGCTTTTCAGCACTGCAGGATTGCTTTTCAGCCCCAAAAGAG CTAGGCTTGACACTAACAATTTTGAGAATCAGCTTCTACTGAAGTTAAAT CTGAGGTTTTACAACTTTGAGTAGCGTGTACTGGCATTAGATTGTCTGTC TTATAGTTTGATAATTAAATACAAACAGTTCTAAAGCAGGATAAAACCTT GTATGCATTTCATTTAATGTTTTTTGAGATTAAAAGCTTAAACAAGA >M13R GGAAACAGCTATGACCATGA >attB4/L4/R4_shared CAACTTTGTATAGAAAAGTTG >attR4 CAACTTTGTATAGAAAAGTTGAACGAGAAACGTAAAATGATATAAATATC AATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAA AACACAACATATCCAGTCACTATGG >ccdB TTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGC GGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTG GCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCAC CGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCA GGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACA TCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAA CTGCAT >ccdB_from_pDEST_R4-R3_(1_mutation) ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTT TGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGA TCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCCCCCGTGAACTT TACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGA TATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCA GCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGA ATATAA >cmR TTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCA TTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCC AGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAA AACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGG TGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAAC CCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGA ATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCG ATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACA CTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGG ATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACT TGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGA ACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATG TTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTT TTTTCTCCAT >attR3 CCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTT TATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCT CGTTCAACTTTATTATACATAGTTG >attB3/L3/R3_shared CAACTTTATTATACA >M13_-20 GTAAAACGACGGCCAGTG >SV40_late_polyA GATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAG AATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTT TATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGC ATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAG CAAGTAAAACCTCTACAAATGTGGTATGGCTGATTAT >Tol2_exon1,_bp1-335_(of_461) ATTTTTTGGAGATCACTTCATTCTATTTTCCCTTGCTATTACCAAACCAA TTGAATTGCGCTGATGCCCAGTTTAATTTAAATGTTATTTATTCTGCCTA TGAAAATCGTTTTCACATTATATGAAATTGGTCAGACATGTTCATTGGTC CTTTGGAAGTGACGTCATGTCACATCTATTACCACAATGCACAGCACCTT GACCTGGAAATTAGGGAAATTATAACAGTCAATCAGTGGAAGAAAATGGA GGAAGTATGTGATTCATCAGCAGCTGCGAGCAGCACAGTCCAAAATCAGC CACAGGATCAAGAGCACCCGTGGCCGTATCTTCGC >517_bp_of_Tol2_5'_end CAGAGGTGTAAAGTACTTGAGTAATTTTACTTGATTACTGTACTTAAGTA TTATTTTTGGGGATTTTTACTTTACTTGAGTACAATTAAAAATCAATACT TTTACTTTTACTTAATTACATTTTTTTAGAAAAAAAAGTACTTTTTACTC CTTACAATTTTATTTACAGTCAAAAAGTACTTATTTTTTGGAGATCACTT CATTCTATTTTCCCTTGCTATTACCAAACCAATTGAATTGCGCTGATGCC CAGTTTAATTTAAATGTTATTTATTCTGCCTATGAAAATCGTTTTCACAT TATATGAAATTGGTCAGACATGTTCATTGGTCCTTTGGAAGTGACGTCAT GTCACATCTATTACCACAATGCACAGCACCTTGACCTGGAAATTAGGGAA ATTATAACAGTCAATCAGTGGAAGAAAATGGAGGAAGTATGTGATTCATC AGCAGCTGCGAGCAGCACAGTCCAAAATCAGCCACAGGATCAAGAGCACC CGTGGCCGTATCTTCGC >5'_TIR CAGAGGTGTAAAGTACTTG >Tyro_frag_ex5 GATCTGCTGGGCTTGCTGAAGGTAGGGGGTCAAGAAC >pBSII_SK+_3’_of_SpeI ACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTCCAGCTTTTGTTCCC TTTAGTGAGGGTTAATTGCGCGCTTGGCGTAATCATGGTCATAGCTGTTT CCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGG AAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACAT TAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGC CAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTAT TGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTC GGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCC ACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCA AAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGG CGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTC CCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCG CCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGG TATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGA ACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGT AACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAA GTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCG CTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCC GGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCA GATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTA CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTC ATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATG AAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTT ACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGT TCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGA GGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCT CACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAG CGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTG TTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACG TTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATG GCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCC CATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCA GAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCAT AATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGA GTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCT CTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTA AAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGAT CTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACT GATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACA GGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTG AATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTT ATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAA ATAGGGGTTCCGCGCACATTTCCCCGAAAAGTG >T3_primer AATTAACCCTCACTAAAGGG >M13R GGAAACAGCTATGACCATGA >pUC_ori TGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCT GGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGAC GCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCG TTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCT TACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC ATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAG CTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATC CGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCAC TGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGT GCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGAC AGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAG TTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTT TTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGA TCCTTTGATCTTTTCTAC >amp ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATT TTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATG CTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGAT GAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACG CCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGT AAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCA ACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCT GAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAA TGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACC ACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTG GAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAC TATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTA AGCATTGG